The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development

Integrins are heterodimeric cell-surface adhesion receptors that play a critical role in tissue development. Characterization of the full-length mRNA encoding the β1 subunit (Itgb1) revealed an alternative functional cleavage and polyadenylation site that yields a new Itgb1 mRNA isoform 578 bp short...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Naipauer, J., Gattelli, A., Degese, M.S., Slomiansky, V., Wertheimer, E., Lamarre, J., Castilla, L., Abba, M., Kordon, E.C., Coso, O.A.
Formato:	JOUR
Materias:	Alternative polyadenylation Gene expression regulation HuR Itgb1 Mammary gland MRNA beta1 integrin caspase 3 dactinomycin HuR protein microRNA RNA isoform short hairpin RNA article breast development cell differentiation consensus sequence differentiation electrophoretic mobility human lactation nonhuman polyadenylation priority journal promoter region protein binding protein cleavage protein expression protein stability RNA transcription Animals Antigens, CD29 Cell Culture Techniques Cell Differentiation Cell Line Data Mining Female Gene Expression Regulation, Developmental Lactation Mammary Glands, Animal Mice Mice, Inbred BALB C Polyadenylation Pregnancy Recombinant Proteins RNA Isoforms RNA Processing, Post-Transcriptional RNA Stability RNA, Messenger RNA, Small Interfering Specific Pathogen-Free Organisms Weaning
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_02646021_v454_n2_p345_Naipauer
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_02646021_v454_n2_p345_Naipauer
record_format	dspace
spelling	todo:paper_02646021_v454_n2_p345_Naipauer2023-10-03T15:13:02Z The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development Naipauer, J. Gattelli, A. Degese, M.S. Slomiansky, V. Wertheimer, E. Lamarre, J. Castilla, L. Abba, M. Kordon, E.C. Coso, O.A. Alternative polyadenylation Gene expression regulation HuR Itgb1 Mammary gland MRNA beta1 integrin caspase 3 dactinomycin HuR protein microRNA RNA isoform short hairpin RNA article breast development cell differentiation consensus sequence differentiation electrophoretic mobility human lactation nonhuman polyadenylation priority journal promoter region protein binding protein cleavage protein expression protein stability RNA transcription Animals Antigens, CD29 Cell Culture Techniques Cell Differentiation Cell Line Data Mining Female Gene Expression Regulation, Developmental Lactation Mammary Glands, Animal Mice Mice, Inbred BALB C Polyadenylation Pregnancy Recombinant Proteins RNA Isoforms RNA Processing, Post-Transcriptional RNA Stability RNA, Messenger RNA, Small Interfering Specific Pathogen-Free Organisms Weaning Integrins are heterodimeric cell-surface adhesion receptors that play a critical role in tissue development. Characterization of the full-length mRNA encoding the β1 subunit (Itgb1) revealed an alternative functional cleavage and polyadenylation site that yields a new Itgb1 mRNA isoform 578 bp shorter than that previously reported. Using a variety of experimental and bioinformatic approaches, we found that the two Itgb1 isoforms are expressed at different levels in a variety of mouse tissues, including the mammary gland, where they are differentially regulated at successive developmental stages. The longer mRNA species is prevelant during lactation, whereas the shorter is induced after weaning. In 3D cultures, where expression of integrin β1 protein is required for normal formation of acini, experimental blockade of the longer isoform induced enhanced expression of the shorter species which allowed normal morphological mammary differentiation. The short isoform lacks AU-rich motifs and miRNA target sequences that are potentially implicated in the regulation of mRNA stability and translation efficiency. We further determined that the AU-binding protein HuR appears to selectively stabilize the longer isoform in the mammary gland. In summary, the results of the present study identify a newregulatory instance involved in the fine-tuning of Itgb1 expression during mammary gland development and function. © 2013 Biochemical Society. Fil:Degese, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Slomiansky, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wertheimer, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kordon, E.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02646021_v454_n2_p345_Naipauer
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Alternative polyadenylation Gene expression regulation HuR Itgb1 Mammary gland MRNA beta1 integrin caspase 3 dactinomycin HuR protein microRNA RNA isoform short hairpin RNA article breast development cell differentiation consensus sequence differentiation electrophoretic mobility human lactation nonhuman polyadenylation priority journal promoter region protein binding protein cleavage protein expression protein stability RNA transcription Animals Antigens, CD29 Cell Culture Techniques Cell Differentiation Cell Line Data Mining Female Gene Expression Regulation, Developmental Lactation Mammary Glands, Animal Mice Mice, Inbred BALB C Polyadenylation Pregnancy Recombinant Proteins RNA Isoforms RNA Processing, Post-Transcriptional RNA Stability RNA, Messenger RNA, Small Interfering Specific Pathogen-Free Organisms Weaning
spellingShingle	Alternative polyadenylation Gene expression regulation HuR Itgb1 Mammary gland MRNA beta1 integrin caspase 3 dactinomycin HuR protein microRNA RNA isoform short hairpin RNA article breast development cell differentiation consensus sequence differentiation electrophoretic mobility human lactation nonhuman polyadenylation priority journal promoter region protein binding protein cleavage protein expression protein stability RNA transcription Animals Antigens, CD29 Cell Culture Techniques Cell Differentiation Cell Line Data Mining Female Gene Expression Regulation, Developmental Lactation Mammary Glands, Animal Mice Mice, Inbred BALB C Polyadenylation Pregnancy Recombinant Proteins RNA Isoforms RNA Processing, Post-Transcriptional RNA Stability RNA, Messenger RNA, Small Interfering Specific Pathogen-Free Organisms Weaning Naipauer, J. Gattelli, A. Degese, M.S. Slomiansky, V. Wertheimer, E. Lamarre, J. Castilla, L. Abba, M. Kordon, E.C. Coso, O.A. The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development
topic_facet	Alternative polyadenylation Gene expression regulation HuR Itgb1 Mammary gland MRNA beta1 integrin caspase 3 dactinomycin HuR protein microRNA RNA isoform short hairpin RNA article breast development cell differentiation consensus sequence differentiation electrophoretic mobility human lactation nonhuman polyadenylation priority journal promoter region protein binding protein cleavage protein expression protein stability RNA transcription Animals Antigens, CD29 Cell Culture Techniques Cell Differentiation Cell Line Data Mining Female Gene Expression Regulation, Developmental Lactation Mammary Glands, Animal Mice Mice, Inbred BALB C Polyadenylation Pregnancy Recombinant Proteins RNA Isoforms RNA Processing, Post-Transcriptional RNA Stability RNA, Messenger RNA, Small Interfering Specific Pathogen-Free Organisms Weaning
description	Integrins are heterodimeric cell-surface adhesion receptors that play a critical role in tissue development. Characterization of the full-length mRNA encoding the β1 subunit (Itgb1) revealed an alternative functional cleavage and polyadenylation site that yields a new Itgb1 mRNA isoform 578 bp shorter than that previously reported. Using a variety of experimental and bioinformatic approaches, we found that the two Itgb1 isoforms are expressed at different levels in a variety of mouse tissues, including the mammary gland, where they are differentially regulated at successive developmental stages. The longer mRNA species is prevelant during lactation, whereas the shorter is induced after weaning. In 3D cultures, where expression of integrin β1 protein is required for normal formation of acini, experimental blockade of the longer isoform induced enhanced expression of the shorter species which allowed normal morphological mammary differentiation. The short isoform lacks AU-rich motifs and miRNA target sequences that are potentially implicated in the regulation of mRNA stability and translation efficiency. We further determined that the AU-binding protein HuR appears to selectively stabilize the longer isoform in the mammary gland. In summary, the results of the present study identify a newregulatory instance involved in the fine-tuning of Itgb1 expression during mammary gland development and function. © 2013 Biochemical Society.
format	JOUR
author	Naipauer, J. Gattelli, A. Degese, M.S. Slomiansky, V. Wertheimer, E. Lamarre, J. Castilla, L. Abba, M. Kordon, E.C. Coso, O.A.
author_facet	Naipauer, J. Gattelli, A. Degese, M.S. Slomiansky, V. Wertheimer, E. Lamarre, J. Castilla, L. Abba, M. Kordon, E.C. Coso, O.A.
author_sort	Naipauer, J.
title	The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development
title_short	The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development
title_full	The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development
title_fullStr	The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development
title_full_unstemmed	The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development
title_sort	use of alternative polyadenylation sites renders integrin β1 (itgb1) mrna isoforms with differential stability during mammary gland development
url	http://hdl.handle.net/20.500.12110/paper_02646021_v454_n2_p345_Naipauer
work_keys_str_mv	AT naipauerj theuseofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT gattellia theuseofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT degesems theuseofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT slomianskyv theuseofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT wertheimere theuseofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT lamarrej theuseofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT castillal theuseofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT abbam theuseofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT kordonec theuseofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT cosooa theuseofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT naipauerj useofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT gattellia useofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT degesems useofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT slomianskyv useofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT wertheimere useofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT lamarrej useofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT castillal useofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT abbam useofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT kordonec useofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment AT cosooa useofalternativepolyadenylationsitesrendersintegrinb1itgb1mrnaisoformswithdifferentialstabilityduringmammaryglanddevelopment
_version_	1782025781827862528

The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development

Ejemplares similares