Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms

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Inositol phospholipids (IPL) from epimastigote forms of Trypanosoma cruzi have been investigated by metabolic labelling with [3H]palmitic acid and by GLC-MS analysis of the lipids obtained from non-labelled parasites. The IPL fraction was separated into phosphatidylinositol (PI) and inositol-phospho...

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Autores principales: Bertello, L.E., Goncalvez, M.F., Colli, W., De Lederkremer, R.M.
Formato: JOUR
Materias:
ceramide
diacylglycerol
fatty acid
glycoprotein
palmitic acid
phosphatidylinositide
phosphatidylinositol
phospholipase C
sphinganine
sphingosine
article
Bacillus thuringiensis
esterification
gas liquid chromatography
lipid analysis
mass spectrometry
nonhuman
priority journal
thin layer chromatography
Trypanosoma cruzi
Trypanosoma
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02646021_v310_n1_p255_Bertello
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_02646021_v310_n1_p255_Bertello
record_format dspace
spelling todo:paper_02646021_v310_n1_p255_Bertello2023-10-03T15:12:56Z Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms Bertello, L.E. Goncalvez, M.F. Colli, W. De Lederkremer, R.M. ceramide diacylglycerol fatty acid glycoprotein palmitic acid phosphatidylinositide phosphatidylinositol phospholipase C sphinganine sphingosine article Bacillus thuringiensis esterification gas liquid chromatography lipid analysis mass spectrometry nonhuman priority journal thin layer chromatography Trypanosoma cruzi Bacillus thuringiensis Trypanosoma Trypanosoma cruzi Inositol phospholipids (IPL) from epimastigote forms of Trypanosoma cruzi have been investigated by metabolic labelling with [3H]palmitic acid and by GLC-MS analysis of the lipids obtained from non-labelled parasites. The IPL fraction was separated into phosphatidylinositol (PI) and inositol-phosphoceramide subfractions, the latter accounting for 80-85% of the total IPL. The neutral lipids released from the IPLs by PI-specific phospholipase C (PI-PLC) from Bacillus thuringiensis were analysed by silica-gel and reverse-phase TLC for the radioactive lipids and by GLC-MS for the non-radioactive samples. Ceramides containing dihydrosphingosine and sphingosine with C(16:0) and C(18:0) fatty acids were identified. The main component in the [3H]palmitic acid-labelled ceramides was palmitoyldihydrosphingosine, while in the non-labelled sample the ceramides contained mainly sphingosine. This could reflect partial uptake of phospholipid from the medium. The PI contain both alkylacyl- and diacyl-glycerol lipids, with the ether lipid being more abundant. The latter was identified as 1-O-hexadecylglycerol esterified by C(18:2) and C(18:1) fatty acids. Interestingly, the same lipid had been identified in the anchor of the 1G7 glycoprotein of T. cruzi metacyclic forms. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02646021_v310_n1_p255_Bertello
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic ceramide
diacylglycerol
fatty acid
glycoprotein
palmitic acid
phosphatidylinositide
phosphatidylinositol
phospholipase C
sphinganine
sphingosine
article
Bacillus thuringiensis
esterification
gas liquid chromatography
lipid analysis
mass spectrometry
nonhuman
priority journal
thin layer chromatography
Trypanosoma cruzi
Bacillus thuringiensis
Trypanosoma
Trypanosoma cruzi
spellingShingle ceramide
diacylglycerol
fatty acid
glycoprotein
palmitic acid
phosphatidylinositide
phosphatidylinositol
phospholipase C
sphinganine
sphingosine
article
Bacillus thuringiensis
esterification
gas liquid chromatography
lipid analysis
mass spectrometry
nonhuman
priority journal
thin layer chromatography
Trypanosoma cruzi
Bacillus thuringiensis
Trypanosoma
Trypanosoma cruzi
Bertello, L.E.
Goncalvez, M.F.
Colli, W.
De Lederkremer, R.M.
Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms
topic_facet ceramide
diacylglycerol
fatty acid
glycoprotein
palmitic acid
phosphatidylinositide
phosphatidylinositol
phospholipase C
sphinganine
sphingosine
article
Bacillus thuringiensis
esterification
gas liquid chromatography
lipid analysis
mass spectrometry
nonhuman
priority journal
thin layer chromatography
Trypanosoma cruzi
Bacillus thuringiensis
Trypanosoma
Trypanosoma cruzi
description Inositol phospholipids (IPL) from epimastigote forms of Trypanosoma cruzi have been investigated by metabolic labelling with [3H]palmitic acid and by GLC-MS analysis of the lipids obtained from non-labelled parasites. The IPL fraction was separated into phosphatidylinositol (PI) and inositol-phosphoceramide subfractions, the latter accounting for 80-85% of the total IPL. The neutral lipids released from the IPLs by PI-specific phospholipase C (PI-PLC) from Bacillus thuringiensis were analysed by silica-gel and reverse-phase TLC for the radioactive lipids and by GLC-MS for the non-radioactive samples. Ceramides containing dihydrosphingosine and sphingosine with C(16:0) and C(18:0) fatty acids were identified. The main component in the [3H]palmitic acid-labelled ceramides was palmitoyldihydrosphingosine, while in the non-labelled sample the ceramides contained mainly sphingosine. This could reflect partial uptake of phospholipid from the medium. The PI contain both alkylacyl- and diacyl-glycerol lipids, with the ether lipid being more abundant. The latter was identified as 1-O-hexadecylglycerol esterified by C(18:2) and C(18:1) fatty acids. Interestingly, the same lipid had been identified in the anchor of the 1G7 glycoprotein of T. cruzi metacyclic forms.
format JOUR
author Bertello, L.E.
Goncalvez, M.F.
Colli, W.
De Lederkremer, R.M.
author_facet Bertello, L.E.
Goncalvez, M.F.
Colli, W.
De Lederkremer, R.M.
author_sort Bertello, L.E.
title Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms
title_short Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms
title_full Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms
title_fullStr Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms
title_full_unstemmed Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms
title_sort structural analysis of inositol phospholipids from trypanosoma cruzi epimastigote forms
url http://hdl.handle.net/20.500.12110/paper_02646021_v310_n1_p255_Bertello
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AT goncalvezmf structuralanalysisofinositolphospholipidsfromtrypanosomacruziepimastigoteforms
AT colliw structuralanalysisofinositolphospholipidsfromtrypanosomacruziepimastigoteforms
AT delederkremerrm structuralanalysisofinositolphospholipidsfromtrypanosomacruziepimastigoteforms
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