Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes

The existence of a GTP-binding protein of the N(s) type in Trypanosoma cruzi was explored. Epimastigote membranes were labelled by cholera toxin in the presence of [adenine-14C]NAD+. After SDS/polyacrylamide-gel electrophoresis of extracted membrane proteins, a single labelled polypeptide band of ap...

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Detalles Bibliográficos
Autores principales: Eisenschlos, C.D., Paladini, A.A., Molina y Vedia, L.
Formato: JOUR
Materias:
cholera toxin
polypeptide
adenylate cyclase
GTP Binding Proteins
guanine nucleotide binding protein
animal cell
electrofusion
epimastigote
membrane
nonhuman
parasite
priority journal
protozoon
Trypanosoma cruzi
article
cell fusion
cell membrane
drug effect
electricity
hybrid cell
lymphoma
metabolism
Animalia
Protozoa
Trypanosoma
Adenylate Cyclase
Cell Fusion
Cell Membrane
Cholera Toxin
Electricity
GTP-Binding Proteins
Hybrid Cells
Lymphoma
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02646021_v237_n3_p913_Eisenschlos
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The existence of a GTP-binding protein of the N(s) type in Trypanosoma cruzi was explored. Epimastigote membranes were labelled by cholera toxin in the presence of [adenine-14C]NAD+. After SDS/polyacrylamide-gel electrophoresis of extracted membrane proteins, a single labelled polypeptide band of apparent M(r) approx. 45000 was detected. Epimastigote cells were treated with N-ethylmaleimide and electrofused to lymphoma S49 cells lacking the N(s) protein. Evidence indicates that in such electrofusion-generated cell hybrids a heterologous adenylate cyclase system was reconstituted with the N(s) protein provided by T. cruzi epimastigotes.

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