Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice

At present, the significance of antibody for protection of the female genital tract against infection with HSV-2 remains controversial. In the present study, the ability of a DNA vaccine encoding different forms of glycoprotein D (gD) of herpes simplex virus-2 (HSV-2) to induce simultaneously cellul...

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Autor principal:	Fló, J.
Formato:	JOUR
Materias:	DNA vaccine Glycoprotein D HSV-2 Mice gamma interferon glycoprotein D herpes simplex vaccine neutralizing antibody animal cell animal experiment animal model antibody blood level antibody response antibody titer article cellular immunity controlled study cytokine release female genetic code herpes simplex Herpes simplex virus 2 humoral immunity immune response immunization lethal dose mouse nonhuman plasmid priority journal solubility spleen vagina virus immunity Animals Antibodies, Viral Antigens, Viral B-Lymphocytes Estrous Cycle Female Genetic Vectors Herpesvirus 2, Human Hypersensitivity, Delayed Immunity, Cellular Immunity, Mucosal Immunization, Secondary Interferon Type II Lymphocyte Activation Mice, Inbred BALB C Mucous Membrane Neutralization Tests Sequence Deletion Spleen T-Lymphocyte Subsets Vaccination Vaccines, DNA Vagina Viral Envelope Proteins Viral Vaccines
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0264410X_v21_n11-12_p1239_Flo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_0264410X_v21_n11-12_p1239_Flo
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spelling	todo:paper_0264410X_v21_n11-12_p1239_Flo2023-10-03T15:12:48Z Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice Fló, J. DNA vaccine Glycoprotein D HSV-2 Mice DNA vaccine gamma interferon glycoprotein D herpes simplex vaccine neutralizing antibody animal cell animal experiment animal model antibody blood level antibody response antibody titer article cellular immunity controlled study cytokine release female genetic code herpes simplex Herpes simplex virus 2 humoral immunity immune response immunization lethal dose mouse nonhuman plasmid priority journal solubility spleen vagina virus immunity Animals Antibodies, Viral Antigens, Viral B-Lymphocytes Estrous Cycle Female Genetic Vectors Herpesvirus 2, Human Hypersensitivity, Delayed Immunity, Cellular Immunity, Mucosal Immunization, Secondary Interferon Type II Lymphocyte Activation Mice Mice, Inbred BALB C Mucous Membrane Neutralization Tests Sequence Deletion Spleen T-Lymphocyte Subsets Vaccination Vaccines, DNA Vagina Viral Envelope Proteins Viral Vaccines At present, the significance of antibody for protection of the female genital tract against infection with HSV-2 remains controversial. In the present study, the ability of a DNA vaccine encoding different forms of glycoprotein D (gD) of herpes simplex virus-2 (HSV-2) to induce simultaneously cellular and humoral responses was evaluated. Mice immunized with a plasmid encoding full length gD (pgD) developed a strong cellular immune response but weak antibody titers in serum and vaginal washings. On the other hand, mice immunized with a plasmid encoding soluble form of gD (pΔgD) showed high titers of antibodies but a very weak cell-mediated immune response. When mice were immunized simultaneously with both plasmids, cellular and humoral immune responses were elicited. This mice showed neutralizing antibodies in serum and vaginal washings as well as a high number of IFN-γ secreting cells in spleen. When challenged with 50 lethal doses of virus, mice immunized with pgD along with pΔgD showed a more complete protection than mice immunized with pgD alone. Collectively these results suggest that neutralizing antibodies help cell-mediated immune response for the protection against HSV-2 infection. © 2002 Elsevier Science Ltd. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0264410X_v21_n11-12_p1239_Flo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	DNA vaccine Glycoprotein D HSV-2 Mice DNA vaccine gamma interferon glycoprotein D herpes simplex vaccine neutralizing antibody animal cell animal experiment animal model antibody blood level antibody response antibody titer article cellular immunity controlled study cytokine release female genetic code herpes simplex Herpes simplex virus 2 humoral immunity immune response immunization lethal dose mouse nonhuman plasmid priority journal solubility spleen vagina virus immunity Animals Antibodies, Viral Antigens, Viral B-Lymphocytes Estrous Cycle Female Genetic Vectors Herpesvirus 2, Human Hypersensitivity, Delayed Immunity, Cellular Immunity, Mucosal Immunization, Secondary Interferon Type II Lymphocyte Activation Mice Mice, Inbred BALB C Mucous Membrane Neutralization Tests Sequence Deletion Spleen T-Lymphocyte Subsets Vaccination Vaccines, DNA Vagina Viral Envelope Proteins Viral Vaccines
spellingShingle	DNA vaccine Glycoprotein D HSV-2 Mice DNA vaccine gamma interferon glycoprotein D herpes simplex vaccine neutralizing antibody animal cell animal experiment animal model antibody blood level antibody response antibody titer article cellular immunity controlled study cytokine release female genetic code herpes simplex Herpes simplex virus 2 humoral immunity immune response immunization lethal dose mouse nonhuman plasmid priority journal solubility spleen vagina virus immunity Animals Antibodies, Viral Antigens, Viral B-Lymphocytes Estrous Cycle Female Genetic Vectors Herpesvirus 2, Human Hypersensitivity, Delayed Immunity, Cellular Immunity, Mucosal Immunization, Secondary Interferon Type II Lymphocyte Activation Mice Mice, Inbred BALB C Mucous Membrane Neutralization Tests Sequence Deletion Spleen T-Lymphocyte Subsets Vaccination Vaccines, DNA Vagina Viral Envelope Proteins Viral Vaccines Fló, J. Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice
topic_facet	DNA vaccine Glycoprotein D HSV-2 Mice DNA vaccine gamma interferon glycoprotein D herpes simplex vaccine neutralizing antibody animal cell animal experiment animal model antibody blood level antibody response antibody titer article cellular immunity controlled study cytokine release female genetic code herpes simplex Herpes simplex virus 2 humoral immunity immune response immunization lethal dose mouse nonhuman plasmid priority journal solubility spleen vagina virus immunity Animals Antibodies, Viral Antigens, Viral B-Lymphocytes Estrous Cycle Female Genetic Vectors Herpesvirus 2, Human Hypersensitivity, Delayed Immunity, Cellular Immunity, Mucosal Immunization, Secondary Interferon Type II Lymphocyte Activation Mice Mice, Inbred BALB C Mucous Membrane Neutralization Tests Sequence Deletion Spleen T-Lymphocyte Subsets Vaccination Vaccines, DNA Vagina Viral Envelope Proteins Viral Vaccines
description	At present, the significance of antibody for protection of the female genital tract against infection with HSV-2 remains controversial. In the present study, the ability of a DNA vaccine encoding different forms of glycoprotein D (gD) of herpes simplex virus-2 (HSV-2) to induce simultaneously cellular and humoral responses was evaluated. Mice immunized with a plasmid encoding full length gD (pgD) developed a strong cellular immune response but weak antibody titers in serum and vaginal washings. On the other hand, mice immunized with a plasmid encoding soluble form of gD (pΔgD) showed high titers of antibodies but a very weak cell-mediated immune response. When mice were immunized simultaneously with both plasmids, cellular and humoral immune responses were elicited. This mice showed neutralizing antibodies in serum and vaginal washings as well as a high number of IFN-γ secreting cells in spleen. When challenged with 50 lethal doses of virus, mice immunized with pgD along with pΔgD showed a more complete protection than mice immunized with pgD alone. Collectively these results suggest that neutralizing antibodies help cell-mediated immune response for the protection against HSV-2 infection. © 2002 Elsevier Science Ltd. All rights reserved.
format	JOUR
author	Fló, J.
author_facet	Fló, J.
author_sort	Fló, J.
title	Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice
title_short	Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice
title_full	Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice
title_fullStr	Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice
title_full_unstemmed	Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice
title_sort	co-immunization with plasmids coding the full length and a soluble form of glycoprotein d of hsv-2 induces protective cellular and humoral immune response in mice
url	http://hdl.handle.net/20.500.12110/paper_0264410X_v21_n11-12_p1239_Flo
work_keys_str_mv	AT floj coimmunizationwithplasmidscodingthefulllengthandasolubleformofglycoproteindofhsv2inducesprotectivecellularandhumoralimmuneresponseinmice
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Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice

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