Prostaglandins and ovarian factors as modulators of the negative inotropic action of histamine in the isolated rat uterus
The present study reports findings on: a) the action of histamine (H) on the spontaneous motility of the uterus isolated from rats during four different stages of the sex cycle; b) the relevance of endogenous and exogenous prostaglandins (PGs) for the inotropic effect of H and c) the relationships b...
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todo:paper_02621746_v16_n3_p267_Viggiano2023-10-03T15:12:35Z Prostaglandins and ovarian factors as modulators of the negative inotropic action of histamine in the isolated rat uterus Viggiano, M. Dveksler, G. Franchi, A.M. Gimeno, M.F. Gimeno, A.L. acetylsalicylic acid histamine prostaglandin prostaglandin e2 prostaglandin f2 alpha animal cell drug efficacy endocrine system estrus female genital system muscle nonhuman ovary priority journal rat smooth muscle uterus Animals Aspirin Depression, Chemical Dinoprost Dinoprostone Dose-Response Relationship, Drug Estrus Female Histamine Pregnancy Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Uterine Contraction Uterus Animalia The present study reports findings on: a) the action of histamine (H) on the spontaneous motility of the uterus isolated from rats during four different stages of the sex cycle; b) the relevance of endogenous and exogenous prostaglandins (PGs) for the inotropic effect of H and c) the relationships between these factors and the generation and release of PGs in rat uterine horns obtained during proestrus, estrus, metestrus and diestrus. Cumulative done-response curves for H, in strips isolated from proestrous rats, showed that the agonist inhibited, at all the concentrations tested, spontaneous myometrial contractions, evoking a maximum effect (100% inhibition) at 10-5M. Incubation with acetylsalicylic acid (ASA; 10-4M) did not modify the dose-response curve for H. In strips from estrous rats, the maximum effect of H was obtained at 10-3M and, after incubating with ASA, the control dose-response curve for H was shifted to the left. A subthreshold dose of PGE2 (10-9M), reversed completely the action of ASA on H uterine responses, whereas PGF2α was not effective. Dose-response curves for H, in uterine strips isolated from metestrous rats, showed the maximum effect (85% of inhibition) at 10-3M. Incubation with ASA, shifted the curve to the left and, the maximum effect increased up to 100% inhibition. A subthreshold dose (10-9M) of PGE2 or of PGF2α shifted to the right the dose-response curve for the amine. In uterine strips isolated from diestrous rats the maximum inotropic action of H evoked only 75% of contractile inhibition and, in the presence the ASA, this influence reached 100%; the dose-response for the agonist being shifted to the left. Both PGE2 or PGF2α, added in the presence of ASA, abolished the displacement evoked by ASA on the control dose-response curve for H. A significant correlation between the tissue output of "PGE-like material" into the bathing solution and the ED50 for the contractile influence of H, was also detected. On the contrary the release of "PGF2α-like material" did not correlate with the ED50 for H. The foregoing results suggest that some endogenous uterine prostaglandins of the 2 series, modulate the depressive inotropic action of H in isolated uterine strips, depending on the ovarian hormonal status of the animals. © 1984. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02621746_v16_n3_p267_Viggiano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
acetylsalicylic acid histamine prostaglandin prostaglandin e2 prostaglandin f2 alpha animal cell drug efficacy endocrine system estrus female genital system muscle nonhuman ovary priority journal rat smooth muscle uterus Animals Aspirin Depression, Chemical Dinoprost Dinoprostone Dose-Response Relationship, Drug Estrus Female Histamine Pregnancy Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Uterine Contraction Uterus Animalia |
| spellingShingle |
acetylsalicylic acid histamine prostaglandin prostaglandin e2 prostaglandin f2 alpha animal cell drug efficacy endocrine system estrus female genital system muscle nonhuman ovary priority journal rat smooth muscle uterus Animals Aspirin Depression, Chemical Dinoprost Dinoprostone Dose-Response Relationship, Drug Estrus Female Histamine Pregnancy Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Uterine Contraction Uterus Animalia Viggiano, M. Dveksler, G. Franchi, A.M. Gimeno, M.F. Gimeno, A.L. Prostaglandins and ovarian factors as modulators of the negative inotropic action of histamine in the isolated rat uterus |
| topic_facet |
acetylsalicylic acid histamine prostaglandin prostaglandin e2 prostaglandin f2 alpha animal cell drug efficacy endocrine system estrus female genital system muscle nonhuman ovary priority journal rat smooth muscle uterus Animals Aspirin Depression, Chemical Dinoprost Dinoprostone Dose-Response Relationship, Drug Estrus Female Histamine Pregnancy Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Uterine Contraction Uterus Animalia |
| description |
The present study reports findings on: a) the action of histamine (H) on the spontaneous motility of the uterus isolated from rats during four different stages of the sex cycle; b) the relevance of endogenous and exogenous prostaglandins (PGs) for the inotropic effect of H and c) the relationships between these factors and the generation and release of PGs in rat uterine horns obtained during proestrus, estrus, metestrus and diestrus. Cumulative done-response curves for H, in strips isolated from proestrous rats, showed that the agonist inhibited, at all the concentrations tested, spontaneous myometrial contractions, evoking a maximum effect (100% inhibition) at 10-5M. Incubation with acetylsalicylic acid (ASA; 10-4M) did not modify the dose-response curve for H. In strips from estrous rats, the maximum effect of H was obtained at 10-3M and, after incubating with ASA, the control dose-response curve for H was shifted to the left. A subthreshold dose of PGE2 (10-9M), reversed completely the action of ASA on H uterine responses, whereas PGF2α was not effective. Dose-response curves for H, in uterine strips isolated from metestrous rats, showed the maximum effect (85% of inhibition) at 10-3M. Incubation with ASA, shifted the curve to the left and, the maximum effect increased up to 100% inhibition. A subthreshold dose (10-9M) of PGE2 or of PGF2α shifted to the right the dose-response curve for the amine. In uterine strips isolated from diestrous rats the maximum inotropic action of H evoked only 75% of contractile inhibition and, in the presence the ASA, this influence reached 100%; the dose-response for the agonist being shifted to the left. Both PGE2 or PGF2α, added in the presence of ASA, abolished the displacement evoked by ASA on the control dose-response curve for H. A significant correlation between the tissue output of "PGE-like material" into the bathing solution and the ED50 for the contractile influence of H, was also detected. On the contrary the release of "PGF2α-like material" did not correlate with the ED50 for H. The foregoing results suggest that some endogenous uterine prostaglandins of the 2 series, modulate the depressive inotropic action of H in isolated uterine strips, depending on the ovarian hormonal status of the animals. © 1984. |
| format |
JOUR |
| author |
Viggiano, M. Dveksler, G. Franchi, A.M. Gimeno, M.F. Gimeno, A.L. |
| author_facet |
Viggiano, M. Dveksler, G. Franchi, A.M. Gimeno, M.F. Gimeno, A.L. |
| author_sort |
Viggiano, M. |
| title |
Prostaglandins and ovarian factors as modulators of the negative inotropic action of histamine in the isolated rat uterus |
| title_short |
Prostaglandins and ovarian factors as modulators of the negative inotropic action of histamine in the isolated rat uterus |
| title_full |
Prostaglandins and ovarian factors as modulators of the negative inotropic action of histamine in the isolated rat uterus |
| title_fullStr |
Prostaglandins and ovarian factors as modulators of the negative inotropic action of histamine in the isolated rat uterus |
| title_full_unstemmed |
Prostaglandins and ovarian factors as modulators of the negative inotropic action of histamine in the isolated rat uterus |
| title_sort |
prostaglandins and ovarian factors as modulators of the negative inotropic action of histamine in the isolated rat uterus |
| url |
http://hdl.handle.net/20.500.12110/paper_02621746_v16_n3_p267_Viggiano |
| work_keys_str_mv |
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| _version_ |
1807323649336672256 |