Influence of light on lignin-degrading activities of fungal genus Polyporus s. str
Six strains belonging to five species of Polyporus (P. arcularius, P. arcularioides, P. tricholoma, P. cfr. tricholoma, and P. varius), collected from an Atlantic Forest area in Misiones (Argentina), where species usually grow exposed to high temperatures and humidity, were identified by morphologic...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0233111X_v58_n11_p947_Grassi |
| Aporte de: |
| id |
todo:paper_0233111X_v58_n11_p947_Grassi |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_0233111X_v58_n11_p947_Grassi2023-10-03T15:11:20Z Influence of light on lignin-degrading activities of fungal genus Polyporus s. str Grassi, E. Robledo, G. Levin, L. light regulation lignin-degrading enzyme production Polyporus wood degradation fungal protein laccase lignin manganese peroxidase peroxidase Argentina chemistry culture medium darkness enzymology fermentation forest fruiting body growth, development and aging light metabolism Polyporus radiation response temperature wood Argentina Culture Media Darkness Fermentation Forests Fruiting Bodies, Fungal Fungal Proteins Laccase Light Lignin Peroxidases Polyporus Temperature Wood Six strains belonging to five species of Polyporus (P. arcularius, P. arcularioides, P. tricholoma, P. cfr. tricholoma, and P. varius), collected from an Atlantic Forest area in Misiones (Argentina), where species usually grow exposed to high temperatures and humidity, were identified by morphological and molecular analyses. P. tricholoma (BAFC 4536) and P. arcularioides (BAFC 4534) were selected by their lignin-degrading enzyme production, their ability to produce primordial of basidiomes under submerged fermentation, and the decrease in lignin content caused in Poplar wood (up to 29% after 45 days). Among several variables evaluated with a Plackett–Burman design (glucose, copper, vanillic acid and manganese concentration, incubation period, and light incidence), the most important factor affecting laccase and Mn-peroxidase (MnP) production by both strains, was light incidence. Light induced fruit body development but diminished laccase and MnP production. Moreover, a modified isoenzymatic laccase pattern was observed, showing additional isoenzymes when fungi were cultivated under darkness and differences in optimal temperature. Although the studied strains did not produce high laccase and MnP titers (uppermost detected 4230 and 90 U L−1, respectively), their laccases showed thermal stability and optimal temperature above 70 °C, representing an interesting source in the search of thermo-tolerant enzymes for biotechnological applications. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0233111X_v58_n11_p947_Grassi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
light regulation lignin-degrading enzyme production Polyporus wood degradation fungal protein laccase lignin manganese peroxidase peroxidase Argentina chemistry culture medium darkness enzymology fermentation forest fruiting body growth, development and aging light metabolism Polyporus radiation response temperature wood Argentina Culture Media Darkness Fermentation Forests Fruiting Bodies, Fungal Fungal Proteins Laccase Light Lignin Peroxidases Polyporus Temperature Wood |
| spellingShingle |
light regulation lignin-degrading enzyme production Polyporus wood degradation fungal protein laccase lignin manganese peroxidase peroxidase Argentina chemistry culture medium darkness enzymology fermentation forest fruiting body growth, development and aging light metabolism Polyporus radiation response temperature wood Argentina Culture Media Darkness Fermentation Forests Fruiting Bodies, Fungal Fungal Proteins Laccase Light Lignin Peroxidases Polyporus Temperature Wood Grassi, E. Robledo, G. Levin, L. Influence of light on lignin-degrading activities of fungal genus Polyporus s. str |
| topic_facet |
light regulation lignin-degrading enzyme production Polyporus wood degradation fungal protein laccase lignin manganese peroxidase peroxidase Argentina chemistry culture medium darkness enzymology fermentation forest fruiting body growth, development and aging light metabolism Polyporus radiation response temperature wood Argentina Culture Media Darkness Fermentation Forests Fruiting Bodies, Fungal Fungal Proteins Laccase Light Lignin Peroxidases Polyporus Temperature Wood |
| description |
Six strains belonging to five species of Polyporus (P. arcularius, P. arcularioides, P. tricholoma, P. cfr. tricholoma, and P. varius), collected from an Atlantic Forest area in Misiones (Argentina), where species usually grow exposed to high temperatures and humidity, were identified by morphological and molecular analyses. P. tricholoma (BAFC 4536) and P. arcularioides (BAFC 4534) were selected by their lignin-degrading enzyme production, their ability to produce primordial of basidiomes under submerged fermentation, and the decrease in lignin content caused in Poplar wood (up to 29% after 45 days). Among several variables evaluated with a Plackett–Burman design (glucose, copper, vanillic acid and manganese concentration, incubation period, and light incidence), the most important factor affecting laccase and Mn-peroxidase (MnP) production by both strains, was light incidence. Light induced fruit body development but diminished laccase and MnP production. Moreover, a modified isoenzymatic laccase pattern was observed, showing additional isoenzymes when fungi were cultivated under darkness and differences in optimal temperature. Although the studied strains did not produce high laccase and MnP titers (uppermost detected 4230 and 90 U L−1, respectively), their laccases showed thermal stability and optimal temperature above 70 °C, representing an interesting source in the search of thermo-tolerant enzymes for biotechnological applications. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
| format |
JOUR |
| author |
Grassi, E. Robledo, G. Levin, L. |
| author_facet |
Grassi, E. Robledo, G. Levin, L. |
| author_sort |
Grassi, E. |
| title |
Influence of light on lignin-degrading activities of fungal genus Polyporus s. str |
| title_short |
Influence of light on lignin-degrading activities of fungal genus Polyporus s. str |
| title_full |
Influence of light on lignin-degrading activities of fungal genus Polyporus s. str |
| title_fullStr |
Influence of light on lignin-degrading activities of fungal genus Polyporus s. str |
| title_full_unstemmed |
Influence of light on lignin-degrading activities of fungal genus Polyporus s. str |
| title_sort |
influence of light on lignin-degrading activities of fungal genus polyporus s. str |
| url |
http://hdl.handle.net/20.500.12110/paper_0233111X_v58_n11_p947_Grassi |
| work_keys_str_mv |
AT grassie influenceoflightonlignindegradingactivitiesoffungalgenuspolyporussstr AT robledog influenceoflightonlignindegradingactivitiesoffungalgenuspolyporussstr AT levinl influenceoflightonlignindegradingactivitiesoffungalgenuspolyporussstr |
| _version_ |
1807322162690785280 |