Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples.
A fast, simple method for the detection of the Escherichia coli polyphosphate kinase (ppk) gene by means of PCR amplification is described. The method uses filters to recover cells from the samples, which makes it suitable for environmental studies. The detection of the ppk gene was achieved from sa...
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Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_02134101_v12_n4_p557_Lopez |
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todo:paper_02134101_v12_n4_p557_Lopez2023-10-03T15:10:07Z Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples. López, N.I. Pettinari, M.J. Méndez, B.S. bacterial DNA phosphotransferase polyphosphate kinase article Escherichia coli genetics microbiology polymerase chain reaction DNA, Bacterial Escherichia coli Phosphotransferases (Phosphate Group Acceptor) Polymerase Chain Reaction Water Microbiology A fast, simple method for the detection of the Escherichia coli polyphosphate kinase (ppk) gene by means of PCR amplification is described. The method uses filters to recover cells from the samples, which makes it suitable for environmental studies. The detection of the ppk gene was achieved from samples containing 10(2) E. coli cells, either in saline solution or in river water. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02134101_v12_n4_p557_Lopez |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
bacterial DNA phosphotransferase polyphosphate kinase article Escherichia coli genetics microbiology polymerase chain reaction DNA, Bacterial Escherichia coli Phosphotransferases (Phosphate Group Acceptor) Polymerase Chain Reaction Water Microbiology |
spellingShingle |
bacterial DNA phosphotransferase polyphosphate kinase article Escherichia coli genetics microbiology polymerase chain reaction DNA, Bacterial Escherichia coli Phosphotransferases (Phosphate Group Acceptor) Polymerase Chain Reaction Water Microbiology López, N.I. Pettinari, M.J. Méndez, B.S. Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples. |
topic_facet |
bacterial DNA phosphotransferase polyphosphate kinase article Escherichia coli genetics microbiology polymerase chain reaction DNA, Bacterial Escherichia coli Phosphotransferases (Phosphate Group Acceptor) Polymerase Chain Reaction Water Microbiology |
description |
A fast, simple method for the detection of the Escherichia coli polyphosphate kinase (ppk) gene by means of PCR amplification is described. The method uses filters to recover cells from the samples, which makes it suitable for environmental studies. The detection of the ppk gene was achieved from samples containing 10(2) E. coli cells, either in saline solution or in river water. |
format |
JOUR |
author |
López, N.I. Pettinari, M.J. Méndez, B.S. |
author_facet |
López, N.I. Pettinari, M.J. Méndez, B.S. |
author_sort |
López, N.I. |
title |
Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples. |
title_short |
Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples. |
title_full |
Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples. |
title_fullStr |
Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples. |
title_full_unstemmed |
Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples. |
title_sort |
monitoring by pcr amplification of the polyphosphate kinase gene added to natural water samples. |
url |
http://hdl.handle.net/20.500.12110/paper_02134101_v12_n4_p557_Lopez |
work_keys_str_mv |
AT lopezni monitoringbypcramplificationofthepolyphosphatekinasegeneaddedtonaturalwatersamples AT pettinarimj monitoringbypcramplificationofthepolyphosphatekinasegeneaddedtonaturalwatersamples AT mendezbs monitoringbypcramplificationofthepolyphosphatekinasegeneaddedtonaturalwatersamples |
_version_ |
1807315871488540672 |