Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples.

A fast, simple method for the detection of the Escherichia coli polyphosphate kinase (ppk) gene by means of PCR amplification is described. The method uses filters to recover cells from the samples, which makes it suitable for environmental studies. The detection of the ppk gene was achieved from sa...

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Autores principales: López, N.I., Pettinari, M.J., Méndez, B.S.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02134101_v12_n4_p557_Lopez
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spelling todo:paper_02134101_v12_n4_p557_Lopez2023-10-03T15:10:07Z Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples. López, N.I. Pettinari, M.J. Méndez, B.S. bacterial DNA phosphotransferase polyphosphate kinase article Escherichia coli genetics microbiology polymerase chain reaction DNA, Bacterial Escherichia coli Phosphotransferases (Phosphate Group Acceptor) Polymerase Chain Reaction Water Microbiology A fast, simple method for the detection of the Escherichia coli polyphosphate kinase (ppk) gene by means of PCR amplification is described. The method uses filters to recover cells from the samples, which makes it suitable for environmental studies. The detection of the ppk gene was achieved from samples containing 10(2) E. coli cells, either in saline solution or in river water. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02134101_v12_n4_p557_Lopez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic bacterial DNA
phosphotransferase
polyphosphate kinase
article
Escherichia coli
genetics
microbiology
polymerase chain reaction
DNA, Bacterial
Escherichia coli
Phosphotransferases (Phosphate Group Acceptor)
Polymerase Chain Reaction
Water Microbiology
spellingShingle bacterial DNA
phosphotransferase
polyphosphate kinase
article
Escherichia coli
genetics
microbiology
polymerase chain reaction
DNA, Bacterial
Escherichia coli
Phosphotransferases (Phosphate Group Acceptor)
Polymerase Chain Reaction
Water Microbiology
López, N.I.
Pettinari, M.J.
Méndez, B.S.
Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples.
topic_facet bacterial DNA
phosphotransferase
polyphosphate kinase
article
Escherichia coli
genetics
microbiology
polymerase chain reaction
DNA, Bacterial
Escherichia coli
Phosphotransferases (Phosphate Group Acceptor)
Polymerase Chain Reaction
Water Microbiology
description A fast, simple method for the detection of the Escherichia coli polyphosphate kinase (ppk) gene by means of PCR amplification is described. The method uses filters to recover cells from the samples, which makes it suitable for environmental studies. The detection of the ppk gene was achieved from samples containing 10(2) E. coli cells, either in saline solution or in river water.
format JOUR
author López, N.I.
Pettinari, M.J.
Méndez, B.S.
author_facet López, N.I.
Pettinari, M.J.
Méndez, B.S.
author_sort López, N.I.
title Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples.
title_short Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples.
title_full Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples.
title_fullStr Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples.
title_full_unstemmed Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples.
title_sort monitoring by pcr amplification of the polyphosphate kinase gene added to natural water samples.
url http://hdl.handle.net/20.500.12110/paper_02134101_v12_n4_p557_Lopez
work_keys_str_mv AT lopezni monitoringbypcramplificationofthepolyphosphatekinasegeneaddedtonaturalwatersamples
AT pettinarimj monitoringbypcramplificationofthepolyphosphatekinasegeneaddedtonaturalwatersamples
AT mendezbs monitoringbypcramplificationofthepolyphosphatekinasegeneaddedtonaturalwatersamples
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