Effect of nitroxyl on the hamster retinal nitridergic pathway
There is a growing body of evidence on the role of nitric oxide (NO) in retinal physiology. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO-), because it is formed by a number of diverse biochemical reactions. The aim of the present...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01970186_v51_n6-7_p424_Saenz |
| Aporte de: |
| id |
todo:paper_01970186_v51_n6-7_p424_Saenz |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_01970186_v51_n6-7_p424_Saenz2023-10-03T15:09:52Z Effect of nitroxyl on the hamster retinal nitridergic pathway Sáenz, D.A. Bari, S.E. Salido, E. Chianelli, M. Rosenstein, R.E. Nitric oxide Nitroxyl Retina amine ammonium derivative arginine cyclic GMP diethylammonium 1 (n,n diethylamino)diazen 1 ium 1,2 diolate glutathione nitric acid derivative nitric oxide nitric oxide synthase nitroxyl s nitrosothiol sodium derivative sodium trioxodinitrate thiol unclassified drug animal cell animal tissue article comparative study concentration (parameters) controlled study enzyme activity lipid peroxidation male nonhuman oxidation reduction potential priority journal regulatory mechanism retina nerve cell Syrian hamster Animals Antioxidants Arginine Cricetinae Cyclic GMP Dose-Response Relationship, Drug Glutathione Lipid Peroxidation Male Mesocricetus Nitrergic Neurons Nitric Oxide Nitric Oxide Synthase Nitrites Nitrogen Oxides Oxidative Stress Quaternary Ammonium Compounds Retina S-Nitrosothiols Signal Transduction Visual Pathways Cricetinae Mesocricetus auratus There is a growing body of evidence on the role of nitric oxide (NO) in retinal physiology. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO-), because it is formed by a number of diverse biochemical reactions. The aim of the present report was to comparatively analyze the effect of HNO and NO on the retinal nitridergic pathway in the golden hamster. For this purpose, sodium trioxodinitrate (Angeli's salt) and diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) were used as HNO and NO releasers, respectively. Angeli's salt and DEA/NO significantly decreased nitric oxide synthase activity. In addition, Angeli's salt (but not DEA/NO) significantly decreased l-arginine uptake. DEA/NO significantly increased cGMP accumulation at low micromolar concentrations, while Angeli's salt affected this parameter with a threshold concentration of 200 μM. Although Angeli's salt and DEA/NO significantly diminished reduced glutathione and protein thiol levels in a similar way, DEA/NO was significantly more effective than AS in increasing S-nitrosothiol levels. None of these compounds increased retinal lipid peroxidation. These results suggest that HNO could regulate the hamster retinal nitridergic pathway by acting through a mechanism that only partly overlaps with that involved in NO response. © 2007 Elsevier Ltd. All rights reserved. Fil:Sáenz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bari, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Chianelli, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rosenstein, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01970186_v51_n6-7_p424_Saenz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Nitric oxide Nitroxyl Retina amine ammonium derivative arginine cyclic GMP diethylammonium 1 (n,n diethylamino)diazen 1 ium 1,2 diolate glutathione nitric acid derivative nitric oxide nitric oxide synthase nitroxyl s nitrosothiol sodium derivative sodium trioxodinitrate thiol unclassified drug animal cell animal tissue article comparative study concentration (parameters) controlled study enzyme activity lipid peroxidation male nonhuman oxidation reduction potential priority journal regulatory mechanism retina nerve cell Syrian hamster Animals Antioxidants Arginine Cricetinae Cyclic GMP Dose-Response Relationship, Drug Glutathione Lipid Peroxidation Male Mesocricetus Nitrergic Neurons Nitric Oxide Nitric Oxide Synthase Nitrites Nitrogen Oxides Oxidative Stress Quaternary Ammonium Compounds Retina S-Nitrosothiols Signal Transduction Visual Pathways Cricetinae Mesocricetus auratus |
| spellingShingle |
Nitric oxide Nitroxyl Retina amine ammonium derivative arginine cyclic GMP diethylammonium 1 (n,n diethylamino)diazen 1 ium 1,2 diolate glutathione nitric acid derivative nitric oxide nitric oxide synthase nitroxyl s nitrosothiol sodium derivative sodium trioxodinitrate thiol unclassified drug animal cell animal tissue article comparative study concentration (parameters) controlled study enzyme activity lipid peroxidation male nonhuman oxidation reduction potential priority journal regulatory mechanism retina nerve cell Syrian hamster Animals Antioxidants Arginine Cricetinae Cyclic GMP Dose-Response Relationship, Drug Glutathione Lipid Peroxidation Male Mesocricetus Nitrergic Neurons Nitric Oxide Nitric Oxide Synthase Nitrites Nitrogen Oxides Oxidative Stress Quaternary Ammonium Compounds Retina S-Nitrosothiols Signal Transduction Visual Pathways Cricetinae Mesocricetus auratus Sáenz, D.A. Bari, S.E. Salido, E. Chianelli, M. Rosenstein, R.E. Effect of nitroxyl on the hamster retinal nitridergic pathway |
| topic_facet |
Nitric oxide Nitroxyl Retina amine ammonium derivative arginine cyclic GMP diethylammonium 1 (n,n diethylamino)diazen 1 ium 1,2 diolate glutathione nitric acid derivative nitric oxide nitric oxide synthase nitroxyl s nitrosothiol sodium derivative sodium trioxodinitrate thiol unclassified drug animal cell animal tissue article comparative study concentration (parameters) controlled study enzyme activity lipid peroxidation male nonhuman oxidation reduction potential priority journal regulatory mechanism retina nerve cell Syrian hamster Animals Antioxidants Arginine Cricetinae Cyclic GMP Dose-Response Relationship, Drug Glutathione Lipid Peroxidation Male Mesocricetus Nitrergic Neurons Nitric Oxide Nitric Oxide Synthase Nitrites Nitrogen Oxides Oxidative Stress Quaternary Ammonium Compounds Retina S-Nitrosothiols Signal Transduction Visual Pathways Cricetinae Mesocricetus auratus |
| description |
There is a growing body of evidence on the role of nitric oxide (NO) in retinal physiology. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO-), because it is formed by a number of diverse biochemical reactions. The aim of the present report was to comparatively analyze the effect of HNO and NO on the retinal nitridergic pathway in the golden hamster. For this purpose, sodium trioxodinitrate (Angeli's salt) and diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) were used as HNO and NO releasers, respectively. Angeli's salt and DEA/NO significantly decreased nitric oxide synthase activity. In addition, Angeli's salt (but not DEA/NO) significantly decreased l-arginine uptake. DEA/NO significantly increased cGMP accumulation at low micromolar concentrations, while Angeli's salt affected this parameter with a threshold concentration of 200 μM. Although Angeli's salt and DEA/NO significantly diminished reduced glutathione and protein thiol levels in a similar way, DEA/NO was significantly more effective than AS in increasing S-nitrosothiol levels. None of these compounds increased retinal lipid peroxidation. These results suggest that HNO could regulate the hamster retinal nitridergic pathway by acting through a mechanism that only partly overlaps with that involved in NO response. © 2007 Elsevier Ltd. All rights reserved. |
| format |
JOUR |
| author |
Sáenz, D.A. Bari, S.E. Salido, E. Chianelli, M. Rosenstein, R.E. |
| author_facet |
Sáenz, D.A. Bari, S.E. Salido, E. Chianelli, M. Rosenstein, R.E. |
| author_sort |
Sáenz, D.A. |
| title |
Effect of nitroxyl on the hamster retinal nitridergic pathway |
| title_short |
Effect of nitroxyl on the hamster retinal nitridergic pathway |
| title_full |
Effect of nitroxyl on the hamster retinal nitridergic pathway |
| title_fullStr |
Effect of nitroxyl on the hamster retinal nitridergic pathway |
| title_full_unstemmed |
Effect of nitroxyl on the hamster retinal nitridergic pathway |
| title_sort |
effect of nitroxyl on the hamster retinal nitridergic pathway |
| url |
http://hdl.handle.net/20.500.12110/paper_01970186_v51_n6-7_p424_Saenz |
| work_keys_str_mv |
AT saenzda effectofnitroxylonthehamsterretinalnitridergicpathway AT barise effectofnitroxylonthehamsterretinalnitridergicpathway AT salidoe effectofnitroxylonthehamsterretinalnitridergicpathway AT chianellim effectofnitroxylonthehamsterretinalnitridergicpathway AT rosensteinre effectofnitroxylonthehamsterretinalnitridergicpathway |
| _version_ |
1807321734934691840 |