Effects of Prolactin on Prostate Androgen Receptors in Male Rats
Five groups of immature (30‐day‐old) intact male rats were injected daily for ten days with different doses of ovine prolactin (5, 10, 25, 50, or 500 μg per rat per day). At the end of the treatment period, the animals were killed, serum was collected for LH and androgens measurements and weights of...
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todo:paper_01963635_v3_n5_p281_BARANAO2023-10-03T15:09:45Z Effects of Prolactin on Prostate Androgen Receptors in Male Rats BARAÑAO, J.S. TESONE, M. OLIVEIRA‐FILHO, R.M. CHIAUZZI, V.A. CALVO, J.C. CHARREAU, E.H. CALANDRA, R.S. androgen receptors immature male rats prolactin prostate Five groups of immature (30‐day‐old) intact male rats were injected daily for ten days with different doses of ovine prolactin (5, 10, 25, 50, or 500 μg per rat per day). At the end of the treatment period, the animals were killed, serum was collected for LH and androgens measurements and weights of testes, prostates, and epididymides were recorded. Available and exchangeable cytoplasmic and total nuclear KC1‐extractable androgen binding sites in the prostate gland were determined using the synthetic androgen R1881 as radioligand. A significant increase in the weights of testes and accessory reproductive glands was observed with the highest dose injected (500 μg per rat per day) as follows: testes, 583 ± 21 (SEM) versus 448 ± 38 mg (P <0.05); prostate, 80.0 ± 4.0 versus 64.7 ± 3.1 mg (P <0.05); epididymis, 72.1 ± 5.0 versus 58.2 ± 4.0 mg (P <0.05). Serum levels of LH were significantly higher in prolactin‐treated animals. Maximal values of serum LH were attained with the dose of 50 μg (99.0 ± 11.2 versus 32.0 ± 4.6 ng/ml; P < 0.05). Increasing doses of prolactin produced a concomitant rise in serum 3α‐androstanediol (2.21 ± 0.45 versus 6.63 ± 0.42 ng/ml in the group treated with 50 μg), whereas testosterone plus dihydrotestosterone levels remained unchanged. A decrease in the concentration of exchangeable cytosolic androgen binding sites in the prostate (fmoleslmg protein) was observed in prolactin injected animals (88.2 ± 0.8 in the control group versus 80.6 ± 0.3 and 74.9 ± 0.3 for doses of 5 and 50 μg, respectively; P <0.05). No changes in androgen binding sites were detected in the group treated with 500 μg per rat per day. On the other hand, prolactin treatment did not alter the number of available androgen binding sites in the prostate gland. Increasing doses of prolactin induced a dose‐related increase in the total number of nuclear KC1‐extractable receptors, expressed per mg DNA or per organ. In addition, the total content of androgen receptors (cytosolic plus nuclear binding sites) was significantly higher in animals treated with 500 μg of prolactin (385 ± 34 versus 228 ± 37 fmoles per organ; P < 0.05). These results suggest that, in addition to the previously described effects of prolactin on Leydig cell responsiveness to LH and on androgen metabolism, this hormone could also modify peripheral LH and androgen levels as well as the androgen receptor content in the rat prostate gland. 1982 American Society of Andrology Fil:BARAÑAO, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:TESONE, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:CALVO, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:CHARREAU, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01963635_v3_n5_p281_BARANAO |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
androgen receptors immature male rats prolactin prostate |
| spellingShingle |
androgen receptors immature male rats prolactin prostate BARAÑAO, J.S. TESONE, M. OLIVEIRA‐FILHO, R.M. CHIAUZZI, V.A. CALVO, J.C. CHARREAU, E.H. CALANDRA, R.S. Effects of Prolactin on Prostate Androgen Receptors in Male Rats |
| topic_facet |
androgen receptors immature male rats prolactin prostate |
| description |
Five groups of immature (30‐day‐old) intact male rats were injected daily for ten days with different doses of ovine prolactin (5, 10, 25, 50, or 500 μg per rat per day). At the end of the treatment period, the animals were killed, serum was collected for LH and androgens measurements and weights of testes, prostates, and epididymides were recorded. Available and exchangeable cytoplasmic and total nuclear KC1‐extractable androgen binding sites in the prostate gland were determined using the synthetic androgen R1881 as radioligand. A significant increase in the weights of testes and accessory reproductive glands was observed with the highest dose injected (500 μg per rat per day) as follows: testes, 583 ± 21 (SEM) versus 448 ± 38 mg (P <0.05); prostate, 80.0 ± 4.0 versus 64.7 ± 3.1 mg (P <0.05); epididymis, 72.1 ± 5.0 versus 58.2 ± 4.0 mg (P <0.05). Serum levels of LH were significantly higher in prolactin‐treated animals. Maximal values of serum LH were attained with the dose of 50 μg (99.0 ± 11.2 versus 32.0 ± 4.6 ng/ml; P < 0.05). Increasing doses of prolactin produced a concomitant rise in serum 3α‐androstanediol (2.21 ± 0.45 versus 6.63 ± 0.42 ng/ml in the group treated with 50 μg), whereas testosterone plus dihydrotestosterone levels remained unchanged. A decrease in the concentration of exchangeable cytosolic androgen binding sites in the prostate (fmoleslmg protein) was observed in prolactin injected animals (88.2 ± 0.8 in the control group versus 80.6 ± 0.3 and 74.9 ± 0.3 for doses of 5 and 50 μg, respectively; P <0.05). No changes in androgen binding sites were detected in the group treated with 500 μg per rat per day. On the other hand, prolactin treatment did not alter the number of available androgen binding sites in the prostate gland. Increasing doses of prolactin induced a dose‐related increase in the total number of nuclear KC1‐extractable receptors, expressed per mg DNA or per organ. In addition, the total content of androgen receptors (cytosolic plus nuclear binding sites) was significantly higher in animals treated with 500 μg of prolactin (385 ± 34 versus 228 ± 37 fmoles per organ; P < 0.05). These results suggest that, in addition to the previously described effects of prolactin on Leydig cell responsiveness to LH and on androgen metabolism, this hormone could also modify peripheral LH and androgen levels as well as the androgen receptor content in the rat prostate gland. 1982 American Society of Andrology |
| format |
JOUR |
| author |
BARAÑAO, J.S. TESONE, M. OLIVEIRA‐FILHO, R.M. CHIAUZZI, V.A. CALVO, J.C. CHARREAU, E.H. CALANDRA, R.S. |
| author_facet |
BARAÑAO, J.S. TESONE, M. OLIVEIRA‐FILHO, R.M. CHIAUZZI, V.A. CALVO, J.C. CHARREAU, E.H. CALANDRA, R.S. |
| author_sort |
BARAÑAO, J.S. |
| title |
Effects of Prolactin on Prostate Androgen Receptors in Male Rats |
| title_short |
Effects of Prolactin on Prostate Androgen Receptors in Male Rats |
| title_full |
Effects of Prolactin on Prostate Androgen Receptors in Male Rats |
| title_fullStr |
Effects of Prolactin on Prostate Androgen Receptors in Male Rats |
| title_full_unstemmed |
Effects of Prolactin on Prostate Androgen Receptors in Male Rats |
| title_sort |
effects of prolactin on prostate androgen receptors in male rats |
| url |
http://hdl.handle.net/20.500.12110/paper_01963635_v3_n5_p281_BARANAO |
| work_keys_str_mv |
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1807320649836789760 |