Hormonal regulation of spermatogenesis in the hypophysectomized rat: FSH maintenance of cellular viability during pubertal spermatogenesis

The potential for follicle-stimulating hormone (FSH) to promote germ- cell survival and the cellular sites of FSH action were studied using a gonadally maturing (pubertal), hypophysectomized (Hx) rat model in which residual testosterone (T) activity was blocked by injections of an androgen- receptor...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Russell, L.D., Kershaw, M., Borg, K.E., El Shennawy, A., Rulli, S.S., Gates, R.J., Calandra, R.S.
Formato:	JOUR
Materias:	Cell viability Hypohysectomy Testis Testosterone androgen receptor flutamide follitropin recombinant follitropin testosterone adolescent animal cell animal experiment animal tissue article cell viability controlled study germ cell hormonal regulation hormone action hypophysectomy male nonhuman priority journal puberty rat seminiferous tubule sertoli cell spermatocyte spermatogenesis testis weight Androgen Antagonists Animals Body Weight Flutamide Follicle Stimulating Hormone Humans Hypophysectomy Male Models, Biological Organ Size Rats Rats, Sprague-Dawley Seminiferous Tubules Sexual Maturation Sperm Count Spermatogenesis
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01963635_v19_n3_p308_Russell
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01963635_v19_n3_p308_Russell
record_format	dspace
spelling	todo:paper_01963635_v19_n3_p308_Russell2023-10-03T15:09:44Z Hormonal regulation of spermatogenesis in the hypophysectomized rat: FSH maintenance of cellular viability during pubertal spermatogenesis Russell, L.D. Kershaw, M. Borg, K.E. El Shennawy, A. Rulli, S.S. Gates, R.J. Calandra, R.S. Cell viability Hypohysectomy Testis Testosterone androgen receptor flutamide follitropin recombinant follitropin testosterone adolescent animal cell animal experiment animal tissue article cell viability controlled study germ cell hormonal regulation hormone action hypophysectomy male nonhuman priority journal puberty rat seminiferous tubule sertoli cell spermatocyte spermatogenesis testis weight Androgen Antagonists Animals Body Weight Flutamide Follicle Stimulating Hormone Humans Hypophysectomy Male Models, Biological Organ Size Rats Rats, Sprague-Dawley Seminiferous Tubules Sexual Maturation Sperm Count Spermatogenesis Testis Testosterone The potential for follicle-stimulating hormone (FSH) to promote germ- cell survival and the cellular sites of FSH action were studied using a gonadally maturing (pubertal), hypophysectomized (Hx) rat model in which residual testosterone (T) activity was blocked by injections of an androgen- receptor antagonist, flutamide. Recombinant human FSH was given to androgen- deprived and androgen-blocked male rats at 27 days of age to determine maintenance of individual germ-cell types at 35 days of age. Follicle- stimulating hormone significantly increased testis weights and tubular diameters as compared with Hx and Hx-flutamide controls, although testis weights in FSH-treated animals were significantly lower than in pituitary- intact animals. Morphometric assays to determine ratios of germ cells to Sertoli cells and to determine the number of germ cells present per hour of development showed that the population of type A spermatogonia in the early stages of the cycle was not responsive to FSH. Follicle-stimulating hormone had a marked ability to maintain cell viability in the rapid, successive divisions that begin in the latter part of the cycle and that continue through the next cycle (i.e., from type A1 to A4 and from intermediate spermatogonia to type B spermatogonia to preleptotene spermatocytes to leptotene/zygotene spermatocytes to young pachytene spermatocytes). The data also suggest T responsiveness of these cell types since the Hx-FSH-flutamide group showed lower cell viability at the aforementioned steps when compared with the Hx-FSH group. Too few cell types were present at subsequent phases of spermatogenesis to allow a sensitive determination of FSH activity in the maintenance of cell viability. The data show the potential of FSH in the absence or relative absence of T activity to maintain cell viability. These data support the concept of overlapping and synergistic (or additive) effects of T and FSH in the immature rat and identify the cellular sites of FSH action. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01963635_v19_n3_p308_Russell
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cell viability Hypohysectomy Testis Testosterone androgen receptor flutamide follitropin recombinant follitropin testosterone adolescent animal cell animal experiment animal tissue article cell viability controlled study germ cell hormonal regulation hormone action hypophysectomy male nonhuman priority journal puberty rat seminiferous tubule sertoli cell spermatocyte spermatogenesis testis weight Androgen Antagonists Animals Body Weight Flutamide Follicle Stimulating Hormone Humans Hypophysectomy Male Models, Biological Organ Size Rats Rats, Sprague-Dawley Seminiferous Tubules Sexual Maturation Sperm Count Spermatogenesis Testis Testosterone
spellingShingle	Cell viability Hypohysectomy Testis Testosterone androgen receptor flutamide follitropin recombinant follitropin testosterone adolescent animal cell animal experiment animal tissue article cell viability controlled study germ cell hormonal regulation hormone action hypophysectomy male nonhuman priority journal puberty rat seminiferous tubule sertoli cell spermatocyte spermatogenesis testis weight Androgen Antagonists Animals Body Weight Flutamide Follicle Stimulating Hormone Humans Hypophysectomy Male Models, Biological Organ Size Rats Rats, Sprague-Dawley Seminiferous Tubules Sexual Maturation Sperm Count Spermatogenesis Testis Testosterone Russell, L.D. Kershaw, M. Borg, K.E. El Shennawy, A. Rulli, S.S. Gates, R.J. Calandra, R.S. Hormonal regulation of spermatogenesis in the hypophysectomized rat: FSH maintenance of cellular viability during pubertal spermatogenesis
topic_facet	Cell viability Hypohysectomy Testis Testosterone androgen receptor flutamide follitropin recombinant follitropin testosterone adolescent animal cell animal experiment animal tissue article cell viability controlled study germ cell hormonal regulation hormone action hypophysectomy male nonhuman priority journal puberty rat seminiferous tubule sertoli cell spermatocyte spermatogenesis testis weight Androgen Antagonists Animals Body Weight Flutamide Follicle Stimulating Hormone Humans Hypophysectomy Male Models, Biological Organ Size Rats Rats, Sprague-Dawley Seminiferous Tubules Sexual Maturation Sperm Count Spermatogenesis Testis Testosterone
description	The potential for follicle-stimulating hormone (FSH) to promote germ- cell survival and the cellular sites of FSH action were studied using a gonadally maturing (pubertal), hypophysectomized (Hx) rat model in which residual testosterone (T) activity was blocked by injections of an androgen- receptor antagonist, flutamide. Recombinant human FSH was given to androgen- deprived and androgen-blocked male rats at 27 days of age to determine maintenance of individual germ-cell types at 35 days of age. Follicle- stimulating hormone significantly increased testis weights and tubular diameters as compared with Hx and Hx-flutamide controls, although testis weights in FSH-treated animals were significantly lower than in pituitary- intact animals. Morphometric assays to determine ratios of germ cells to Sertoli cells and to determine the number of germ cells present per hour of development showed that the population of type A spermatogonia in the early stages of the cycle was not responsive to FSH. Follicle-stimulating hormone had a marked ability to maintain cell viability in the rapid, successive divisions that begin in the latter part of the cycle and that continue through the next cycle (i.e., from type A1 to A4 and from intermediate spermatogonia to type B spermatogonia to preleptotene spermatocytes to leptotene/zygotene spermatocytes to young pachytene spermatocytes). The data also suggest T responsiveness of these cell types since the Hx-FSH-flutamide group showed lower cell viability at the aforementioned steps when compared with the Hx-FSH group. Too few cell types were present at subsequent phases of spermatogenesis to allow a sensitive determination of FSH activity in the maintenance of cell viability. The data show the potential of FSH in the absence or relative absence of T activity to maintain cell viability. These data support the concept of overlapping and synergistic (or additive) effects of T and FSH in the immature rat and identify the cellular sites of FSH action.
format	JOUR
author	Russell, L.D. Kershaw, M. Borg, K.E. El Shennawy, A. Rulli, S.S. Gates, R.J. Calandra, R.S.
author_facet	Russell, L.D. Kershaw, M. Borg, K.E. El Shennawy, A. Rulli, S.S. Gates, R.J. Calandra, R.S.
author_sort	Russell, L.D.
title	Hormonal regulation of spermatogenesis in the hypophysectomized rat: FSH maintenance of cellular viability during pubertal spermatogenesis
title_short	Hormonal regulation of spermatogenesis in the hypophysectomized rat: FSH maintenance of cellular viability during pubertal spermatogenesis
title_full	Hormonal regulation of spermatogenesis in the hypophysectomized rat: FSH maintenance of cellular viability during pubertal spermatogenesis
title_fullStr	Hormonal regulation of spermatogenesis in the hypophysectomized rat: FSH maintenance of cellular viability during pubertal spermatogenesis
title_full_unstemmed	Hormonal regulation of spermatogenesis in the hypophysectomized rat: FSH maintenance of cellular viability during pubertal spermatogenesis
title_sort	hormonal regulation of spermatogenesis in the hypophysectomized rat: fsh maintenance of cellular viability during pubertal spermatogenesis
url	http://hdl.handle.net/20.500.12110/paper_01963635_v19_n3_p308_Russell
work_keys_str_mv	AT russellld hormonalregulationofspermatogenesisinthehypophysectomizedratfshmaintenanceofcellularviabilityduringpubertalspermatogenesis AT kershawm hormonalregulationofspermatogenesisinthehypophysectomizedratfshmaintenanceofcellularviabilityduringpubertalspermatogenesis AT borgke hormonalregulationofspermatogenesisinthehypophysectomizedratfshmaintenanceofcellularviabilityduringpubertalspermatogenesis AT elshennawya hormonalregulationofspermatogenesisinthehypophysectomizedratfshmaintenanceofcellularviabilityduringpubertalspermatogenesis AT rulliss hormonalregulationofspermatogenesisinthehypophysectomizedratfshmaintenanceofcellularviabilityduringpubertalspermatogenesis AT gatesrj hormonalregulationofspermatogenesisinthehypophysectomizedratfshmaintenanceofcellularviabilityduringpubertalspermatogenesis AT calandrars hormonalregulationofspermatogenesisinthehypophysectomizedratfshmaintenanceofcellularviabilityduringpubertalspermatogenesis
_version_	1807316769172357120

Hormonal regulation of spermatogenesis in the hypophysectomized rat: FSH maintenance of cellular viability during pubertal spermatogenesis

Ejemplares similares