Both orexin receptors are expressed in rat ovaries and fluctuate with the estrous cycle: Effects of orexin receptor antagonists on gonadotropins and ovulation
Orexins are peptides controlling feeding, sleep, and neuroendocrine functions. They are synthesized by the hypothalamus with projections throughout the brain. Orexins and their orexin 1 (OX1) and orexin 2 receptors (OX2) are present outside the central nervous system. Here the expression of preproor...
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todo:paper_01931849_v293_n4_pE977_Silveyra2023-10-03T15:09:15Z Both orexin receptors are expressed in rat ovaries and fluctuate with the estrous cycle: Effects of orexin receptor antagonists on gonadotropins and ovulation Silveyra, P. Lux-Lantos, V. Libertun, C. Orexin 1 and orexin 2 receptor antagonists Ovary cetrorelix gonadotropin jnj 10397049 orexin orexin 1 receptor orexin 2 receptor pentobarbital preproorexin receptor blocking agent sb 334867 a unclassified drug animal experiment animal tissue article bleeding controlled study estrus cycle female food intake gonadotropin blood level histology hyperemia light dark cycle nonhuman ovulation priority journal proestrus protein expression quantitative assay rat real time polymerase chain reaction reverse transcription polymerase chain reaction Sprague Dawley rat Animals Benzoxazoles Dioxanes Eating Estrous Cycle Female Gene Expression Regulation Gonadotropin-Releasing Hormone Gonadotropins Intracellular Signaling Peptides and Proteins Neuropeptides Ovary Ovulation Pentobarbital Phenylurea Compounds Rats Rats, Sprague-Dawley Receptors, G-Protein-Coupled Receptors, Neuropeptide RNA, Messenger Urea Orexins are peptides controlling feeding, sleep, and neuroendocrine functions. They are synthesized by the hypothalamus with projections throughout the brain. Orexins and their orexin 1 (OX1) and orexin 2 receptors (OX2) are present outside the central nervous system. Here the expression of preproorexin (PPO), OX1, and OX2 was studied in rat ovaries. PPO, OX1, and OX2 were determined by quantitative real-time RT-PCR in ovaries of cycling Sprague-Dawley rats on all days of the cycle. Serum hormones and food consumption were determined. Ovarian OX1 and OX2 expression was then studied after ovulation blockade with Cetrorelix or Nembutal. Finally, proestrous rats were treated at 1400 and 1900 with a selective OX1 antagonist (SB-334867-A) and/or a selective OX2 antagonist (JNJ-10397049), and hormone levels, ovulation, and ovarian histology were studied. Both receptors' expression increased in the ovary between 1700 and 2300 of proestrus exclusively, in coincidence with hormone peaks, but not with the dark-light cycle or food intake. PPO was not detected. Cetrorelix or Nembutal prevented the increases of OX1 and OX2 while blunting gonadotropin peaks. SB-334867-A and JNJ-10397049, alone or combined, decreased serum gonadotropins and reduced ova number the following morning; ovaries showed a bloody (hyperemic and/or hemorrhagic) reaction with more preovulatory follicles and less corpora lutea. Here we demonstrate for the first time an increased ovarian expression of both OX1 and OX2, only during proestrous afternoon, and its hormone dependence but not dependence on the dark-light cycle. Two new receptor antagonists reduced proestrous gonadotropins and/or ova number while producing ovarian structural changes. Copyright © 2007 the American Physiological Society. Fil:Silveyra, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01931849_v293_n4_pE977_Silveyra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Orexin 1 and orexin 2 receptor antagonists Ovary cetrorelix gonadotropin jnj 10397049 orexin orexin 1 receptor orexin 2 receptor pentobarbital preproorexin receptor blocking agent sb 334867 a unclassified drug animal experiment animal tissue article bleeding controlled study estrus cycle female food intake gonadotropin blood level histology hyperemia light dark cycle nonhuman ovulation priority journal proestrus protein expression quantitative assay rat real time polymerase chain reaction reverse transcription polymerase chain reaction Sprague Dawley rat Animals Benzoxazoles Dioxanes Eating Estrous Cycle Female Gene Expression Regulation Gonadotropin-Releasing Hormone Gonadotropins Intracellular Signaling Peptides and Proteins Neuropeptides Ovary Ovulation Pentobarbital Phenylurea Compounds Rats Rats, Sprague-Dawley Receptors, G-Protein-Coupled Receptors, Neuropeptide RNA, Messenger Urea |
| spellingShingle |
Orexin 1 and orexin 2 receptor antagonists Ovary cetrorelix gonadotropin jnj 10397049 orexin orexin 1 receptor orexin 2 receptor pentobarbital preproorexin receptor blocking agent sb 334867 a unclassified drug animal experiment animal tissue article bleeding controlled study estrus cycle female food intake gonadotropin blood level histology hyperemia light dark cycle nonhuman ovulation priority journal proestrus protein expression quantitative assay rat real time polymerase chain reaction reverse transcription polymerase chain reaction Sprague Dawley rat Animals Benzoxazoles Dioxanes Eating Estrous Cycle Female Gene Expression Regulation Gonadotropin-Releasing Hormone Gonadotropins Intracellular Signaling Peptides and Proteins Neuropeptides Ovary Ovulation Pentobarbital Phenylurea Compounds Rats Rats, Sprague-Dawley Receptors, G-Protein-Coupled Receptors, Neuropeptide RNA, Messenger Urea Silveyra, P. Lux-Lantos, V. Libertun, C. Both orexin receptors are expressed in rat ovaries and fluctuate with the estrous cycle: Effects of orexin receptor antagonists on gonadotropins and ovulation |
| topic_facet |
Orexin 1 and orexin 2 receptor antagonists Ovary cetrorelix gonadotropin jnj 10397049 orexin orexin 1 receptor orexin 2 receptor pentobarbital preproorexin receptor blocking agent sb 334867 a unclassified drug animal experiment animal tissue article bleeding controlled study estrus cycle female food intake gonadotropin blood level histology hyperemia light dark cycle nonhuman ovulation priority journal proestrus protein expression quantitative assay rat real time polymerase chain reaction reverse transcription polymerase chain reaction Sprague Dawley rat Animals Benzoxazoles Dioxanes Eating Estrous Cycle Female Gene Expression Regulation Gonadotropin-Releasing Hormone Gonadotropins Intracellular Signaling Peptides and Proteins Neuropeptides Ovary Ovulation Pentobarbital Phenylurea Compounds Rats Rats, Sprague-Dawley Receptors, G-Protein-Coupled Receptors, Neuropeptide RNA, Messenger Urea |
| description |
Orexins are peptides controlling feeding, sleep, and neuroendocrine functions. They are synthesized by the hypothalamus with projections throughout the brain. Orexins and their orexin 1 (OX1) and orexin 2 receptors (OX2) are present outside the central nervous system. Here the expression of preproorexin (PPO), OX1, and OX2 was studied in rat ovaries. PPO, OX1, and OX2 were determined by quantitative real-time RT-PCR in ovaries of cycling Sprague-Dawley rats on all days of the cycle. Serum hormones and food consumption were determined. Ovarian OX1 and OX2 expression was then studied after ovulation blockade with Cetrorelix or Nembutal. Finally, proestrous rats were treated at 1400 and 1900 with a selective OX1 antagonist (SB-334867-A) and/or a selective OX2 antagonist (JNJ-10397049), and hormone levels, ovulation, and ovarian histology were studied. Both receptors' expression increased in the ovary between 1700 and 2300 of proestrus exclusively, in coincidence with hormone peaks, but not with the dark-light cycle or food intake. PPO was not detected. Cetrorelix or Nembutal prevented the increases of OX1 and OX2 while blunting gonadotropin peaks. SB-334867-A and JNJ-10397049, alone or combined, decreased serum gonadotropins and reduced ova number the following morning; ovaries showed a bloody (hyperemic and/or hemorrhagic) reaction with more preovulatory follicles and less corpora lutea. Here we demonstrate for the first time an increased ovarian expression of both OX1 and OX2, only during proestrous afternoon, and its hormone dependence but not dependence on the dark-light cycle. Two new receptor antagonists reduced proestrous gonadotropins and/or ova number while producing ovarian structural changes. Copyright © 2007 the American Physiological Society. |
| format |
JOUR |
| author |
Silveyra, P. Lux-Lantos, V. Libertun, C. |
| author_facet |
Silveyra, P. Lux-Lantos, V. Libertun, C. |
| author_sort |
Silveyra, P. |
| title |
Both orexin receptors are expressed in rat ovaries and fluctuate with the estrous cycle: Effects of orexin receptor antagonists on gonadotropins and ovulation |
| title_short |
Both orexin receptors are expressed in rat ovaries and fluctuate with the estrous cycle: Effects of orexin receptor antagonists on gonadotropins and ovulation |
| title_full |
Both orexin receptors are expressed in rat ovaries and fluctuate with the estrous cycle: Effects of orexin receptor antagonists on gonadotropins and ovulation |
| title_fullStr |
Both orexin receptors are expressed in rat ovaries and fluctuate with the estrous cycle: Effects of orexin receptor antagonists on gonadotropins and ovulation |
| title_full_unstemmed |
Both orexin receptors are expressed in rat ovaries and fluctuate with the estrous cycle: Effects of orexin receptor antagonists on gonadotropins and ovulation |
| title_sort |
both orexin receptors are expressed in rat ovaries and fluctuate with the estrous cycle: effects of orexin receptor antagonists on gonadotropins and ovulation |
| url |
http://hdl.handle.net/20.500.12110/paper_01931849_v293_n4_pE977_Silveyra |
| work_keys_str_mv |
AT silveyrap bothorexinreceptorsareexpressedinratovariesandfluctuatewiththeestrouscycleeffectsoforexinreceptorantagonistsongonadotropinsandovulation AT luxlantosv bothorexinreceptorsareexpressedinratovariesandfluctuatewiththeestrouscycleeffectsoforexinreceptorantagonistsongonadotropinsandovulation AT libertunc bothorexinreceptorsareexpressedinratovariesandfluctuatewiththeestrouscycleeffectsoforexinreceptorantagonistsongonadotropinsandovulation |
| _version_ |
1807321413675122688 |