Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells

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Activation of pituitary angiotensin (ANG II) type 1 receptors (AT1) mobilizes intracellular Ca2+, resulting in increased prolactin secretion. We first assessed desensitization of AT1 receptors by testing ANG II-induced intracellular Ca2+ concentration ([Ca2+]i) response in rat anterior pituitary cel...

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Autores principales: Iglesias, A.G., Suärez, C., Feierstein, C., Díaz-Torga, G., Becu-Villalobos, D., Becü-Villalobos, D.
Formato: JOUR
Materias:
Angiotensin type 1 receptor
Calcium
Homologous desensitization
Protein kinase C
Thyrotropin-releasing hormone
angiotensin
angiotensin 1 receptor
calcium ion
inositol trisphosphate
ionomycin
prolactin
protein kinase C
protirelin
animal cell
animal experiment
article
calcium cell level
calcium mobilization
concentration response
controlled study
desensitization
enzyme activation
female
hypophysis cell
nonhuman
priority journal
prolactin release
Angiotensin II
Animals
Cells, Cultured
Drug Tolerance
Female
Inositol 1,4,5-Trisphosphate
Ionomycin
Pituitary Gland, Anterior
Prolactin
Protein Kinase C
Rats
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 1
Receptor, Angiotensin, Type 2
Receptors, Angiotensin
Tetradecanoylphorbol Acetate
Thyrotropin-Releasing Hormone
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01931849_v280_n343-3_pE462_Iglesias
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_01931849_v280_n343-3_pE462_Iglesias2023-10-03T15:09:13Z Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells Iglesias, A.G. Suärez, C. Feierstein, C. Díaz-Torga, G. Becu-Villalobos, D. Becü-Villalobos, D. Angiotensin type 1 receptor Calcium Homologous desensitization Protein kinase C Thyrotropin-releasing hormone angiotensin angiotensin 1 receptor calcium ion inositol trisphosphate ionomycin prolactin protein kinase C protirelin animal cell animal experiment article calcium cell level calcium mobilization concentration response controlled study desensitization enzyme activation female hypophysis cell nonhuman priority journal prolactin release Angiotensin II Animals Calcium Cells, Cultured Drug Tolerance Female Inositol 1,4,5-Trisphosphate Ionomycin Pituitary Gland, Anterior Prolactin Protein Kinase C Rats Rats, Sprague-Dawley Receptor, Angiotensin, Type 1 Receptor, Angiotensin, Type 2 Receptors, Angiotensin Tetradecanoylphorbol Acetate Thyrotropin-Releasing Hormone Activation of pituitary angiotensin (ANG II) type 1 receptors (AT1) mobilizes intracellular Ca2+, resulting in increased prolactin secretion. We first assessed desensitization of AT1 receptors by testing ANG II-induced intracellular Ca2+ concentration ([Ca2+]i) response in rat anterior pituitary cells. A period as short as 1 min with 10-7 M ANG II was effective in producing desensitization (remaining response was 66.8 ± 2.1% of nondesensitized cells). Desensitization was a concentration-related event (EC50: 1.1 nM). Although partial recovery was obtained 15 min after removal of ANG II, full response could not be achieved even after 4 h (77.6 ± 2.4%). Experiments with 5 × 10-7 M ionomycin indicated that intracellular Ca2+ stores of desensitized cells had already recovered when desensitization was still significant. The thyrotropin-releasing hormone (TRH)-induced intracellular Ca2+ peak was attenuated in the ANG II-pretreated group. ANG II pretreatment also desensitized ANG II- and TRH-induced inositol phosphate generation (72.8 ± 3.5 and 69.6 ± 6.1%, respectively, for inositol triphosphate) and prolactin secretion (53.4 ± 2.3 and 65.1 ± 7.2%), effects independent of PKC activation. We conclude that, in pituitary cells, inositol triphosphate formation, [Ca2+]i mobilization, and prolactin release in response to ANG II undergo rapid, long-lasting, homologous and heterologous desensitization. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01931849_v280_n343-3_pE462_Iglesias
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Angiotensin type 1 receptor
Calcium
Homologous desensitization
Protein kinase C
Thyrotropin-releasing hormone
angiotensin
angiotensin 1 receptor
calcium ion
inositol trisphosphate
ionomycin
prolactin
protein kinase C
protirelin
animal cell
animal experiment
article
calcium cell level
calcium mobilization
concentration response
controlled study
desensitization
enzyme activation
female
hypophysis cell
nonhuman
priority journal
prolactin release
Angiotensin II
Animals
Calcium
Cells, Cultured
Drug Tolerance
Female
Inositol 1,4,5-Trisphosphate
Ionomycin
Pituitary Gland, Anterior
Prolactin
Protein Kinase C
Rats
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 1
Receptor, Angiotensin, Type 2
Receptors, Angiotensin
Tetradecanoylphorbol Acetate
Thyrotropin-Releasing Hormone
spellingShingle Angiotensin type 1 receptor
Calcium
Homologous desensitization
Protein kinase C
Thyrotropin-releasing hormone
angiotensin
angiotensin 1 receptor
calcium ion
inositol trisphosphate
ionomycin
prolactin
protein kinase C
protirelin
animal cell
animal experiment
article
calcium cell level
calcium mobilization
concentration response
controlled study
desensitization
enzyme activation
female
hypophysis cell
nonhuman
priority journal
prolactin release
Angiotensin II
Animals
Calcium
Cells, Cultured
Drug Tolerance
Female
Inositol 1,4,5-Trisphosphate
Ionomycin
Pituitary Gland, Anterior
Prolactin
Protein Kinase C
Rats
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 1
Receptor, Angiotensin, Type 2
Receptors, Angiotensin
Tetradecanoylphorbol Acetate
Thyrotropin-Releasing Hormone
Iglesias, A.G.
Suärez, C.
Feierstein, C.
Díaz-Torga, G.
Becu-Villalobos, D.
Becü-Villalobos, D.
Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells
topic_facet Angiotensin type 1 receptor
Calcium
Homologous desensitization
Protein kinase C
Thyrotropin-releasing hormone
angiotensin
angiotensin 1 receptor
calcium ion
inositol trisphosphate
ionomycin
prolactin
protein kinase C
protirelin
animal cell
animal experiment
article
calcium cell level
calcium mobilization
concentration response
controlled study
desensitization
enzyme activation
female
hypophysis cell
nonhuman
priority journal
prolactin release
Angiotensin II
Animals
Calcium
Cells, Cultured
Drug Tolerance
Female
Inositol 1,4,5-Trisphosphate
Ionomycin
Pituitary Gland, Anterior
Prolactin
Protein Kinase C
Rats
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 1
Receptor, Angiotensin, Type 2
Receptors, Angiotensin
Tetradecanoylphorbol Acetate
Thyrotropin-Releasing Hormone
description Activation of pituitary angiotensin (ANG II) type 1 receptors (AT1) mobilizes intracellular Ca2+, resulting in increased prolactin secretion. We first assessed desensitization of AT1 receptors by testing ANG II-induced intracellular Ca2+ concentration ([Ca2+]i) response in rat anterior pituitary cells. A period as short as 1 min with 10-7 M ANG II was effective in producing desensitization (remaining response was 66.8 ± 2.1% of nondesensitized cells). Desensitization was a concentration-related event (EC50: 1.1 nM). Although partial recovery was obtained 15 min after removal of ANG II, full response could not be achieved even after 4 h (77.6 ± 2.4%). Experiments with 5 × 10-7 M ionomycin indicated that intracellular Ca2+ stores of desensitized cells had already recovered when desensitization was still significant. The thyrotropin-releasing hormone (TRH)-induced intracellular Ca2+ peak was attenuated in the ANG II-pretreated group. ANG II pretreatment also desensitized ANG II- and TRH-induced inositol phosphate generation (72.8 ± 3.5 and 69.6 ± 6.1%, respectively, for inositol triphosphate) and prolactin secretion (53.4 ± 2.3 and 65.1 ± 7.2%), effects independent of PKC activation. We conclude that, in pituitary cells, inositol triphosphate formation, [Ca2+]i mobilization, and prolactin release in response to ANG II undergo rapid, long-lasting, homologous and heterologous desensitization.
format JOUR
author Iglesias, A.G.
Suärez, C.
Feierstein, C.
Díaz-Torga, G.
Becu-Villalobos, D.
Becü-Villalobos, D.
author_facet Iglesias, A.G.
Suärez, C.
Feierstein, C.
Díaz-Torga, G.
Becu-Villalobos, D.
Becü-Villalobos, D.
author_sort Iglesias, A.G.
title Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells
title_short Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells
title_full Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells
title_fullStr Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells
title_full_unstemmed Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells
title_sort desensitization of angiotensin ii: effect on [ca2+]i, inositol triphosphate, and prolactin in pituitary cells
url http://hdl.handle.net/20.500.12110/paper_01931849_v280_n343-3_pE462_Iglesias
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AT suarezc desensitizationofangiotensiniieffectonca2iinositoltriphosphateandprolactininpituitarycells
AT feiersteinc desensitizationofangiotensiniieffectonca2iinositoltriphosphateandprolactininpituitarycells
AT diaztorgag desensitizationofangiotensiniieffectonca2iinositoltriphosphateandprolactininpituitarycells
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