Immunological detection of a GarV-Type virus in Argentine garlic cultivars
A cDNA library representing the genomes of several garlic viruses was generated using a viral RNA mixture as template. Analysis of randomly selected clones allowed the identification of different vital genomic sequences. On the basis of amino acid and nucleotide sequence comparisons, one of them was...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01912917_v81_n9_p1005_Helguera |
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todo:paper_01912917_v81_n9_p1005_Helguera2023-10-03T15:08:57Z Immunological detection of a GarV-Type virus in Argentine garlic cultivars Helguera, M. Bravo-Almonacid, F. Kobayashi, K. Rabinowicz, P.D. Conci, V. Mentaberry, A. Acari Allium cepa Allium porrum Allium sativum Argentina (fish) Carlavirus Dianthus caryophyllus Escherichia Garlic mite-borne filamentous virus Garlic virus A Leek yellow stripe potyvirus Potyvirus Acari Allexivirus Allium cepa Allium porrum Allium sativum Carlavirus Dianthus caryophyllus Escherichia coli Garlic mite-borne filamentous virus Garlic virus A Leek yellow stripe virus Onion yellow dwarf virus Potyvirus A cDNA library representing the genomes of several garlic viruses was generated using a viral RNA mixture as template. Analysis of randomly selected clones allowed the identification of different vital genomic sequences. On the basis of amino acid and nucleotide sequence comparisons, one of them was assigned to garlic virus A (GarV-A), a novel flexuous, rod-shaped virus recently reported in Japan. The coat protein (CP) of this virus was expressed in Escherichia colt cells and used as immunogen to produce polyclonal antibodies. The expression protein was recognized by an antiserum detecting garlic mite-borne filamentous virus, but did not react with antibodies specific for other garlic viruses. Antibodies raised against the vital CP reacted with extracts infected with garlic mite-borne viruses and fail to recognize preparations of onion yellow dwarf petyvirus, leek yellow stripe potyvirus, and carnation latent carlavirus. The same antibodies decorated vital particles exhibiting a modal length of 586 nm in immuno electron microscopy with decoration assays. Double-antibody enzyme-linked immunosorbent assays and tissue printing assays performed with these antibodies allowed detection of GarV-A in most garlic cultivars used in Argentina. Fil:Kobayashi, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rabinowicz, P.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mentaberry, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01912917_v81_n9_p1005_Helguera |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acari Allium cepa Allium porrum Allium sativum Argentina (fish) Carlavirus Dianthus caryophyllus Escherichia Garlic mite-borne filamentous virus Garlic virus A Leek yellow stripe potyvirus Potyvirus Acari Allexivirus Allium cepa Allium porrum Allium sativum Carlavirus Dianthus caryophyllus Escherichia coli Garlic mite-borne filamentous virus Garlic virus A Leek yellow stripe virus Onion yellow dwarf virus Potyvirus |
| spellingShingle |
Acari Allium cepa Allium porrum Allium sativum Argentina (fish) Carlavirus Dianthus caryophyllus Escherichia Garlic mite-borne filamentous virus Garlic virus A Leek yellow stripe potyvirus Potyvirus Acari Allexivirus Allium cepa Allium porrum Allium sativum Carlavirus Dianthus caryophyllus Escherichia coli Garlic mite-borne filamentous virus Garlic virus A Leek yellow stripe virus Onion yellow dwarf virus Potyvirus Helguera, M. Bravo-Almonacid, F. Kobayashi, K. Rabinowicz, P.D. Conci, V. Mentaberry, A. Immunological detection of a GarV-Type virus in Argentine garlic cultivars |
| topic_facet |
Acari Allium cepa Allium porrum Allium sativum Argentina (fish) Carlavirus Dianthus caryophyllus Escherichia Garlic mite-borne filamentous virus Garlic virus A Leek yellow stripe potyvirus Potyvirus Acari Allexivirus Allium cepa Allium porrum Allium sativum Carlavirus Dianthus caryophyllus Escherichia coli Garlic mite-borne filamentous virus Garlic virus A Leek yellow stripe virus Onion yellow dwarf virus Potyvirus |
| description |
A cDNA library representing the genomes of several garlic viruses was generated using a viral RNA mixture as template. Analysis of randomly selected clones allowed the identification of different vital genomic sequences. On the basis of amino acid and nucleotide sequence comparisons, one of them was assigned to garlic virus A (GarV-A), a novel flexuous, rod-shaped virus recently reported in Japan. The coat protein (CP) of this virus was expressed in Escherichia colt cells and used as immunogen to produce polyclonal antibodies. The expression protein was recognized by an antiserum detecting garlic mite-borne filamentous virus, but did not react with antibodies specific for other garlic viruses. Antibodies raised against the vital CP reacted with extracts infected with garlic mite-borne viruses and fail to recognize preparations of onion yellow dwarf petyvirus, leek yellow stripe potyvirus, and carnation latent carlavirus. The same antibodies decorated vital particles exhibiting a modal length of 586 nm in immuno electron microscopy with decoration assays. Double-antibody enzyme-linked immunosorbent assays and tissue printing assays performed with these antibodies allowed detection of GarV-A in most garlic cultivars used in Argentina. |
| format |
JOUR |
| author |
Helguera, M. Bravo-Almonacid, F. Kobayashi, K. Rabinowicz, P.D. Conci, V. Mentaberry, A. |
| author_facet |
Helguera, M. Bravo-Almonacid, F. Kobayashi, K. Rabinowicz, P.D. Conci, V. Mentaberry, A. |
| author_sort |
Helguera, M. |
| title |
Immunological detection of a GarV-Type virus in Argentine garlic cultivars |
| title_short |
Immunological detection of a GarV-Type virus in Argentine garlic cultivars |
| title_full |
Immunological detection of a GarV-Type virus in Argentine garlic cultivars |
| title_fullStr |
Immunological detection of a GarV-Type virus in Argentine garlic cultivars |
| title_full_unstemmed |
Immunological detection of a GarV-Type virus in Argentine garlic cultivars |
| title_sort |
immunological detection of a garv-type virus in argentine garlic cultivars |
| url |
http://hdl.handle.net/20.500.12110/paper_01912917_v81_n9_p1005_Helguera |
| work_keys_str_mv |
AT helgueram immunologicaldetectionofagarvtypevirusinargentinegarliccultivars AT bravoalmonacidf immunologicaldetectionofagarvtypevirusinargentinegarliccultivars AT kobayashik immunologicaldetectionofagarvtypevirusinargentinegarliccultivars AT rabinowiczpd immunologicaldetectionofagarvtypevirusinargentinegarliccultivars AT conciv immunologicaldetectionofagarvtypevirusinargentinegarliccultivars AT mentaberrya immunologicaldetectionofagarvtypevirusinargentinegarliccultivars |
| _version_ |
1807322819050078208 |