Functional characterization of a sucrose:Fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance

We have previously reported the molecular characterization of a putative sucrose:fructan 6-fructosyltransferase (6-SFT) of Bromus pictus, a graminean species from Patagonia, tolerant to cold and drought. Here, this enzyme was functionally characterized by heterologous expression in Pichia pastoris a...

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Detalles Bibliográficos
Autores principales: del Viso, F., Casabuono, A.C., Couto, A.S., Hopp, H.E., Puebla, A.F., Heinz, R.A.
Formato: JOUR
Materias:
6-SFT
Bromus pictus
Freezing tolerance
Fructans
Pichia pastoris
Tobacco
Bromus
Graminea
Hordeum
Nicotiana obtusifolia
Nicotiana tabacum
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01761617_v168_n5_p493_delViso
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01761617_v168_n5_p493_delViso
record_format dspace
spelling todo:paper_01761617_v168_n5_p493_delViso2023-10-03T15:08:02Z Functional characterization of a sucrose:Fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance del Viso, F. Casabuono, A.C. Couto, A.S. Hopp, H.E. Puebla, A.F. Heinz, R.A. 6-SFT Bromus pictus Freezing tolerance Fructans Pichia pastoris Tobacco Bromus Bromus pictus Graminea Hordeum Nicotiana obtusifolia Nicotiana tabacum Pichia pastoris We have previously reported the molecular characterization of a putative sucrose:fructan 6-fructosyltransferase (6-SFT) of Bromus pictus, a graminean species from Patagonia, tolerant to cold and drought. Here, this enzyme was functionally characterized by heterologous expression in Pichia pastoris and Nicotiana tabacum. Recombinant P. pastoris Bp6-SFT showed comparable characteristics to barley 6-SFT and an evident fructosyltransferase activity synthesizing bifurcose from sucrose and 1-kestotriose. Transgenic tobacco plants expressing Bp6-SFT, showed fructosyltransferase activity and fructan accumulation in leaves. Bp6-SFT plants exposed to freezing conditions showed a significantly lower electrolyte leakage in leaves compared to control plants, indicating less membrane damage. Concomitantly these transgenic plants resumed growth more rapidly than control ones. These results indicate that Bp6-SFT transgenic tobacco plants that accumulate fructan showed enhanced freezing tolerance compared to control plants. © 2010 Elsevier GmbH. Fil:del Viso, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Casabuono, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Puebla, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Heinz, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01761617_v168_n5_p493_delViso
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 6-SFT
Bromus pictus
Freezing tolerance
Fructans
Pichia pastoris
Tobacco
Bromus
Bromus pictus
Graminea
Hordeum
Nicotiana obtusifolia
Nicotiana tabacum
Pichia pastoris
spellingShingle 6-SFT
Bromus pictus
Freezing tolerance
Fructans
Pichia pastoris
Tobacco
Bromus
Bromus pictus
Graminea
Hordeum
Nicotiana obtusifolia
Nicotiana tabacum
Pichia pastoris
del Viso, F.
Casabuono, A.C.
Couto, A.S.
Hopp, H.E.
Puebla, A.F.
Heinz, R.A.
Functional characterization of a sucrose:Fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance
topic_facet 6-SFT
Bromus pictus
Freezing tolerance
Fructans
Pichia pastoris
Tobacco
Bromus
Bromus pictus
Graminea
Hordeum
Nicotiana obtusifolia
Nicotiana tabacum
Pichia pastoris
description We have previously reported the molecular characterization of a putative sucrose:fructan 6-fructosyltransferase (6-SFT) of Bromus pictus, a graminean species from Patagonia, tolerant to cold and drought. Here, this enzyme was functionally characterized by heterologous expression in Pichia pastoris and Nicotiana tabacum. Recombinant P. pastoris Bp6-SFT showed comparable characteristics to barley 6-SFT and an evident fructosyltransferase activity synthesizing bifurcose from sucrose and 1-kestotriose. Transgenic tobacco plants expressing Bp6-SFT, showed fructosyltransferase activity and fructan accumulation in leaves. Bp6-SFT plants exposed to freezing conditions showed a significantly lower electrolyte leakage in leaves compared to control plants, indicating less membrane damage. Concomitantly these transgenic plants resumed growth more rapidly than control ones. These results indicate that Bp6-SFT transgenic tobacco plants that accumulate fructan showed enhanced freezing tolerance compared to control plants. © 2010 Elsevier GmbH.
format JOUR
author del Viso, F.
Casabuono, A.C.
Couto, A.S.
Hopp, H.E.
Puebla, A.F.
Heinz, R.A.
author_facet del Viso, F.
Casabuono, A.C.
Couto, A.S.
Hopp, H.E.
Puebla, A.F.
Heinz, R.A.
author_sort del Viso, F.
title Functional characterization of a sucrose:Fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance
title_short Functional characterization of a sucrose:Fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance
title_full Functional characterization of a sucrose:Fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance
title_fullStr Functional characterization of a sucrose:Fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance
title_full_unstemmed Functional characterization of a sucrose:Fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance
title_sort functional characterization of a sucrose:fructan 6-fructosyltransferase of the cold-resistant grass bromus pictus by heterelogous expression in pichia pastoris and nicotiana tabacum and its involvement in freezing tolerance
url http://hdl.handle.net/20.500.12110/paper_01761617_v168_n5_p493_delViso
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