trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production
A Bacillus megaterium genomic fragment, which encoded an activator homologous to σ54 regulators and which was capable of activating Escherichia coli ato genes in trans, was detected in a gene library of B. megaterium screened for β-ketothiolase activity. The fragment presented only one complete open...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01757598_v49_n6_p737_Pettinari |
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todo:paper_01757598_v49_n6_p737_Pettinari2023-10-03T15:07:54Z trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production Pettinari, M.J. Vazquez, G.J. Krüger, N. Vary, P.S. Steinbüchel, A. Méndez, B.S. acetyl coenzyme a acetyltransferase polyhydroxyalkanoic acid regulator protein article bacillus megaterium enzyme activity escherichia coli fermentation gene activation gene library gene transfer open reading frame operon recombinant plasmid regulator gene structural gene technique Amino Acid Sequence Bacillus megaterium Escherichia coli Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Open Reading Frames Plasmids Polyesters Sequence Alignment Sequence Homology, Amino Acid Trans-Activation (Genetics) Bacillus megaterium Escherichia coli Negibacteria Posibacteria Prokaryota A Bacillus megaterium genomic fragment, which encoded an activator homologous to σ54 regulators and which was capable of activating Escherichia coli ato genes in trans, was detected in a gene library of B. megaterium screened for β-ketothiolase activity. The fragment presented only one complete open reading frame (ORF1), which encoded a protein of 398 amino acids, The recombinant plasmid complemented mutations in the Escherichia coli atoC regulatory gene. The constitutive expression of the E. coli ato operon mediated by ORF1 could be useful for the synthesis of polyhydroxyalkanoates with different flexibility properties by recombinant E. coli strains. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vazquez, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01757598_v49_n6_p737_Pettinari |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
acetyl coenzyme a acetyltransferase polyhydroxyalkanoic acid regulator protein article bacillus megaterium enzyme activity escherichia coli fermentation gene activation gene library gene transfer open reading frame operon recombinant plasmid regulator gene structural gene technique Amino Acid Sequence Bacillus megaterium Escherichia coli Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Open Reading Frames Plasmids Polyesters Sequence Alignment Sequence Homology, Amino Acid Trans-Activation (Genetics) Bacillus megaterium Escherichia coli Negibacteria Posibacteria Prokaryota |
| spellingShingle |
acetyl coenzyme a acetyltransferase polyhydroxyalkanoic acid regulator protein article bacillus megaterium enzyme activity escherichia coli fermentation gene activation gene library gene transfer open reading frame operon recombinant plasmid regulator gene structural gene technique Amino Acid Sequence Bacillus megaterium Escherichia coli Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Open Reading Frames Plasmids Polyesters Sequence Alignment Sequence Homology, Amino Acid Trans-Activation (Genetics) Bacillus megaterium Escherichia coli Negibacteria Posibacteria Prokaryota Pettinari, M.J. Vazquez, G.J. Krüger, N. Vary, P.S. Steinbüchel, A. Méndez, B.S. trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production |
| topic_facet |
acetyl coenzyme a acetyltransferase polyhydroxyalkanoic acid regulator protein article bacillus megaterium enzyme activity escherichia coli fermentation gene activation gene library gene transfer open reading frame operon recombinant plasmid regulator gene structural gene technique Amino Acid Sequence Bacillus megaterium Escherichia coli Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Open Reading Frames Plasmids Polyesters Sequence Alignment Sequence Homology, Amino Acid Trans-Activation (Genetics) Bacillus megaterium Escherichia coli Negibacteria Posibacteria Prokaryota |
| description |
A Bacillus megaterium genomic fragment, which encoded an activator homologous to σ54 regulators and which was capable of activating Escherichia coli ato genes in trans, was detected in a gene library of B. megaterium screened for β-ketothiolase activity. The fragment presented only one complete open reading frame (ORF1), which encoded a protein of 398 amino acids, The recombinant plasmid complemented mutations in the Escherichia coli atoC regulatory gene. The constitutive expression of the E. coli ato operon mediated by ORF1 could be useful for the synthesis of polyhydroxyalkanoates with different flexibility properties by recombinant E. coli strains. |
| format |
JOUR |
| author |
Pettinari, M.J. Vazquez, G.J. Krüger, N. Vary, P.S. Steinbüchel, A. Méndez, B.S. |
| author_facet |
Pettinari, M.J. Vazquez, G.J. Krüger, N. Vary, P.S. Steinbüchel, A. Méndez, B.S. |
| author_sort |
Pettinari, M.J. |
| title |
trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production |
| title_short |
trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production |
| title_full |
trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production |
| title_fullStr |
trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production |
| title_full_unstemmed |
trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production |
| title_sort |
trans activation of the escherichia coli ato structural genes by a regulatory protein from bacillus megaterium: potential use in polyhydroxyalkanoate production |
| url |
http://hdl.handle.net/20.500.12110/paper_01757598_v49_n6_p737_Pettinari |
| work_keys_str_mv |
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1807320190303600640 |