Characterization of prophages containing “evolved” Dit/Tal modules in the genome of Lactobacillus casei BL23

Lactic acid bacteria (LAB) have many applications in food and industrial fermentations. Prophage induction and generation of new virulent phages is a risk for the dairy industry. We identified three complete prophages (PLE1, PLE2, and PLE3) in the genome of the well-studied probiotic strain Lactobac...

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Autores principales:	Dieterle, M.E., Fina Martin, J., Durán, R., Nemirovsky, S.I., Sanchez Rivas, C., Bowman, C., Russell, D., Hatfull, G.F., Cambillau, C., Piuri, M.
Formato:	JOUR
Materias:	Bacteriophage Baseplate Lactobacillus casei Prophage Bacteriophages Bins Dairy products Genes Lactic acid Molecular biology Polymerase chain reaction Strain Carbohydrate-binding modules Industrial fermentation Lactic acid bacteria Prophages Quantitative real time PCR Receptor-binding proteins Proteins bacterial protein distal tail protein mitomycin tail associated lysin baseplate protein unclassified drug nucleic acid synthesis inhibitor viral protein bacteriophage bacterium chemical binding fermentation food industry genetic analysis genome protein Article bacterial genome bacterial strain controlled study gene sequence kinetics Lactobacillus Lactobacillus casei bl23 Lactobacillus paracasei Lactococcus Listeria nonhuman prophage real time polymerase chain reaction receptor binding Streptococcus structural proteomics food control genetics metabolism physiology virology virus activation Food Microbiology Mitomycin Nucleic Acid Synthesis Inhibitors Viral Tail Proteins Virus Activation
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01757598_v100_n21_p9201_Dieterle
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01757598_v100_n21_p9201_Dieterle
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spelling	todo:paper_01757598_v100_n21_p9201_Dieterle2023-10-03T15:07:53Z Characterization of prophages containing “evolved” Dit/Tal modules in the genome of Lactobacillus casei BL23 Dieterle, M.E. Fina Martin, J. Durán, R. Nemirovsky, S.I. Sanchez Rivas, C. Bowman, C. Russell, D. Hatfull, G.F. Cambillau, C. Piuri, M. Bacteriophage Baseplate Lactobacillus casei Prophage Bacteriophages Bins Dairy products Genes Lactic acid Molecular biology Polymerase chain reaction Strain Baseplate Carbohydrate-binding modules Industrial fermentation Lactic acid bacteria Lactobacillus casei Prophages Quantitative real time PCR Receptor-binding proteins Proteins bacterial protein distal tail protein mitomycin tail associated lysin baseplate protein unclassified drug nucleic acid synthesis inhibitor viral protein bacteriophage bacterium chemical binding fermentation food industry genetic analysis genome protein Article bacterial genome bacterial strain controlled study gene sequence kinetics Lactobacillus Lactobacillus casei Lactobacillus casei bl23 Lactobacillus paracasei Lactococcus Listeria nonhuman prophage real time polymerase chain reaction receptor binding Streptococcus structural proteomics food control genetics Lactobacillus casei metabolism physiology prophage virology virus activation Lactobacillus Lactobacillus casei Lactococcus Listeria Streptococcus Food Microbiology Lactobacillus casei Mitomycin Nucleic Acid Synthesis Inhibitors Prophages Viral Tail Proteins Virus Activation Lactic acid bacteria (LAB) have many applications in food and industrial fermentations. Prophage induction and generation of new virulent phages is a risk for the dairy industry. We identified three complete prophages (PLE1, PLE2, and PLE3) in the genome of the well-studied probiotic strain Lactobacillus casei BL23. All of them have mosaic architectures with homologous sequences to Streptococcus, Lactococcus, Lactobacillus, and Listeria phages or strains. Using a combination of quantitative real-time PCR, genomics, and proteomics, we showed that PLE2 and PLE3 can be induced—but with different kinetics—in the presence of mitomycin C, although PLE1 remains as a prophage. A structural analysis of the distal tail (Dit) and tail associated lysin (Tal) baseplate proteins of these prophages and other L. casei/paracasei phages and prophages provides evidence that carbohydrate-binding modules (CBM) located within these “evolved” proteins may replace receptor binding proteins (RBPs) present in other well-studied LAB phages. The detailed study of prophage induction in this prototype strain in combination with characterization of the proteins involved in host recognition will facilitate the design of new strategies for avoiding phage propagation in the dairy industry. © 2016, Springer-Verlag Berlin Heidelberg. Fil:Nemirovsky, S.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Piuri, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01757598_v100_n21_p9201_Dieterle
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bacteriophage Baseplate Lactobacillus casei Prophage Bacteriophages Bins Dairy products Genes Lactic acid Molecular biology Polymerase chain reaction Strain Baseplate Carbohydrate-binding modules Industrial fermentation Lactic acid bacteria Lactobacillus casei Prophages Quantitative real time PCR Receptor-binding proteins Proteins bacterial protein distal tail protein mitomycin tail associated lysin baseplate protein unclassified drug nucleic acid synthesis inhibitor viral protein bacteriophage bacterium chemical binding fermentation food industry genetic analysis genome protein Article bacterial genome bacterial strain controlled study gene sequence kinetics Lactobacillus Lactobacillus casei Lactobacillus casei bl23 Lactobacillus paracasei Lactococcus Listeria nonhuman prophage real time polymerase chain reaction receptor binding Streptococcus structural proteomics food control genetics Lactobacillus casei metabolism physiology prophage virology virus activation Lactobacillus Lactobacillus casei Lactococcus Listeria Streptococcus Food Microbiology Lactobacillus casei Mitomycin Nucleic Acid Synthesis Inhibitors Prophages Viral Tail Proteins Virus Activation
spellingShingle	Bacteriophage Baseplate Lactobacillus casei Prophage Bacteriophages Bins Dairy products Genes Lactic acid Molecular biology Polymerase chain reaction Strain Baseplate Carbohydrate-binding modules Industrial fermentation Lactic acid bacteria Lactobacillus casei Prophages Quantitative real time PCR Receptor-binding proteins Proteins bacterial protein distal tail protein mitomycin tail associated lysin baseplate protein unclassified drug nucleic acid synthesis inhibitor viral protein bacteriophage bacterium chemical binding fermentation food industry genetic analysis genome protein Article bacterial genome bacterial strain controlled study gene sequence kinetics Lactobacillus Lactobacillus casei Lactobacillus casei bl23 Lactobacillus paracasei Lactococcus Listeria nonhuman prophage real time polymerase chain reaction receptor binding Streptococcus structural proteomics food control genetics Lactobacillus casei metabolism physiology prophage virology virus activation Lactobacillus Lactobacillus casei Lactococcus Listeria Streptococcus Food Microbiology Lactobacillus casei Mitomycin Nucleic Acid Synthesis Inhibitors Prophages Viral Tail Proteins Virus Activation Dieterle, M.E. Fina Martin, J. Durán, R. Nemirovsky, S.I. Sanchez Rivas, C. Bowman, C. Russell, D. Hatfull, G.F. Cambillau, C. Piuri, M. Characterization of prophages containing “evolved” Dit/Tal modules in the genome of Lactobacillus casei BL23
topic_facet	Bacteriophage Baseplate Lactobacillus casei Prophage Bacteriophages Bins Dairy products Genes Lactic acid Molecular biology Polymerase chain reaction Strain Baseplate Carbohydrate-binding modules Industrial fermentation Lactic acid bacteria Lactobacillus casei Prophages Quantitative real time PCR Receptor-binding proteins Proteins bacterial protein distal tail protein mitomycin tail associated lysin baseplate protein unclassified drug nucleic acid synthesis inhibitor viral protein bacteriophage bacterium chemical binding fermentation food industry genetic analysis genome protein Article bacterial genome bacterial strain controlled study gene sequence kinetics Lactobacillus Lactobacillus casei Lactobacillus casei bl23 Lactobacillus paracasei Lactococcus Listeria nonhuman prophage real time polymerase chain reaction receptor binding Streptococcus structural proteomics food control genetics Lactobacillus casei metabolism physiology prophage virology virus activation Lactobacillus Lactobacillus casei Lactococcus Listeria Streptococcus Food Microbiology Lactobacillus casei Mitomycin Nucleic Acid Synthesis Inhibitors Prophages Viral Tail Proteins Virus Activation
description	Lactic acid bacteria (LAB) have many applications in food and industrial fermentations. Prophage induction and generation of new virulent phages is a risk for the dairy industry. We identified three complete prophages (PLE1, PLE2, and PLE3) in the genome of the well-studied probiotic strain Lactobacillus casei BL23. All of them have mosaic architectures with homologous sequences to Streptococcus, Lactococcus, Lactobacillus, and Listeria phages or strains. Using a combination of quantitative real-time PCR, genomics, and proteomics, we showed that PLE2 and PLE3 can be induced—but with different kinetics—in the presence of mitomycin C, although PLE1 remains as a prophage. A structural analysis of the distal tail (Dit) and tail associated lysin (Tal) baseplate proteins of these prophages and other L. casei/paracasei phages and prophages provides evidence that carbohydrate-binding modules (CBM) located within these “evolved” proteins may replace receptor binding proteins (RBPs) present in other well-studied LAB phages. The detailed study of prophage induction in this prototype strain in combination with characterization of the proteins involved in host recognition will facilitate the design of new strategies for avoiding phage propagation in the dairy industry. © 2016, Springer-Verlag Berlin Heidelberg.
format	JOUR
author	Dieterle, M.E. Fina Martin, J. Durán, R. Nemirovsky, S.I. Sanchez Rivas, C. Bowman, C. Russell, D. Hatfull, G.F. Cambillau, C. Piuri, M.
author_facet	Dieterle, M.E. Fina Martin, J. Durán, R. Nemirovsky, S.I. Sanchez Rivas, C. Bowman, C. Russell, D. Hatfull, G.F. Cambillau, C. Piuri, M.
author_sort	Dieterle, M.E.
title	Characterization of prophages containing “evolved” Dit/Tal modules in the genome of Lactobacillus casei BL23
title_short	Characterization of prophages containing “evolved” Dit/Tal modules in the genome of Lactobacillus casei BL23
title_full	Characterization of prophages containing “evolved” Dit/Tal modules in the genome of Lactobacillus casei BL23
title_fullStr	Characterization of prophages containing “evolved” Dit/Tal modules in the genome of Lactobacillus casei BL23
title_full_unstemmed	Characterization of prophages containing “evolved” Dit/Tal modules in the genome of Lactobacillus casei BL23
title_sort	characterization of prophages containing “evolved” dit/tal modules in the genome of lactobacillus casei bl23
url	http://hdl.handle.net/20.500.12110/paper_01757598_v100_n21_p9201_Dieterle
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Characterization of prophages containing “evolved” Dit/Tal modules in the genome of Lactobacillus casei BL23

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