Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356

In this work, we studied the role of surface layer (S-layer) proteins in the adaptation of Lactobacillus acidophilus ATCC 4356 to the osmotic stress generated by high salt. The amounts of the predominant and the auxiliary S-layer proteins SlpA and SlpX were strongly influenced by the growth phase an...

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Autores principales:	Palomino, M.M., Waehner, P.M., Fina Martin, J., Ojeda, P., Malone, L., Sánchez Rivas, C., Prado Acosta, M., Allievi, M.C., Ruzal, S.M.
Formato:	JOUR
Materias:	Lactobacillus Osmotic stress S-layer proteins Bacilli Gene expression Osmosis Proteins Sodium chloride Cell-wall thickness Lactobacillus acidophilus Lactobacillus casei Lipoteichoic acids Stress condition Biosynthesis envelope protein lipoteichoic acid messenger RNA peptidoglycan probiotic agent protein signal peptide sodium chloride surface layer protein a surface layer protein x unclassified drug membrane protein adaptation bacterium gene expression genetic analysis hypersaline environment osmosis physiological response RNA Article cell wall controlled study gene expression profiling genetic regulation genome analysis growth curve human osmotic stress protein modification protein synthesis salt stress surface property drug effects genetics metabolism osmotic pressure Gene Expression Membrane Glycoproteins Osmotic Pressure Sodium Chloride
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01757598_v100_n19_p8475_Palomino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_01757598_v100_n19_p8475_Palomino2023-10-03T15:07:52Z Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356 Palomino, M.M. Waehner, P.M. Fina Martin, J. Ojeda, P. Malone, L. Sánchez Rivas, C. Prado Acosta, M. Allievi, M.C. Ruzal, S.M. Lactobacillus Osmotic stress S-layer proteins Bacilli Gene expression Osmosis Proteins Sodium chloride Cell-wall thickness Lactobacillus Lactobacillus acidophilus Lactobacillus casei Lipoteichoic acids Osmotic stress S-layer proteins Stress condition Biosynthesis envelope protein lipoteichoic acid messenger RNA peptidoglycan probiotic agent protein signal peptide sodium chloride surface layer protein a surface layer protein x unclassified drug membrane protein S-layer proteins adaptation bacterium gene expression genetic analysis hypersaline environment osmosis physiological response protein RNA adaptation Article cell wall controlled study gene expression profiling genetic regulation genome analysis growth curve human Lactobacillus acidophilus Lactobacillus casei osmotic stress protein modification protein synthesis salt stress surface property drug effects gene expression genetics Lactobacillus acidophilus metabolism osmotic pressure Lactobacillus Lactobacillus acidophilus Lactobacillus casei Gene Expression Lactobacillus acidophilus Membrane Glycoproteins Osmotic Pressure Sodium Chloride In this work, we studied the role of surface layer (S-layer) proteins in the adaptation of Lactobacillus acidophilus ATCC 4356 to the osmotic stress generated by high salt. The amounts of the predominant and the auxiliary S-layer proteins SlpA and SlpX were strongly influenced by the growth phase and high-salt conditions (0.6 M NaCl). Changes in gene expression were also observed as the mRNAs of the slpA and slpX genes increased related to the growth phase and presence of high salt. A growth stage-dependent modification on the S-layer protein profile in response to NaCl was observed: while in control conditions, the auxiliary SlpX protein represented less than 10 % of the total S-layer protein, in high-salt conditions, it increased to almost 40 % in the stationary phase. The increase in S-layer protein synthesis in the stress condition could be a consequence of or a way to counteract the fragility of the cell wall, since a decrease in the cell wall thickness and envelope components (peptidoglycan layer and lipoteichoic acid content) was observed in L. acidophilus when compared to a non-S-layer-producing species such as Lactobacillus casei. Also, the stationary phase and growth in high-salt medium resulted in increased release of S-layer proteins to the supernatant medium. Overall, these findings suggest that pre-growth in high-salt conditions would result in an advantage for the probiotic nature of L. acidophilus ATCC 4356 as the increased amount and release of the S-layer might be appropriate for its antimicrobial capacity. © 2016, Springer-Verlag Berlin Heidelberg. Fil:Palomino, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ojeda, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Prado Acosta, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Allievi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01757598_v100_n19_p8475_Palomino
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Lactobacillus Osmotic stress S-layer proteins Bacilli Gene expression Osmosis Proteins Sodium chloride Cell-wall thickness Lactobacillus Lactobacillus acidophilus Lactobacillus casei Lipoteichoic acids Osmotic stress S-layer proteins Stress condition Biosynthesis envelope protein lipoteichoic acid messenger RNA peptidoglycan probiotic agent protein signal peptide sodium chloride surface layer protein a surface layer protein x unclassified drug membrane protein S-layer proteins adaptation bacterium gene expression genetic analysis hypersaline environment osmosis physiological response protein RNA adaptation Article cell wall controlled study gene expression profiling genetic regulation genome analysis growth curve human Lactobacillus acidophilus Lactobacillus casei osmotic stress protein modification protein synthesis salt stress surface property drug effects gene expression genetics Lactobacillus acidophilus metabolism osmotic pressure Lactobacillus Lactobacillus acidophilus Lactobacillus casei Gene Expression Lactobacillus acidophilus Membrane Glycoproteins Osmotic Pressure Sodium Chloride
spellingShingle	Lactobacillus Osmotic stress S-layer proteins Bacilli Gene expression Osmosis Proteins Sodium chloride Cell-wall thickness Lactobacillus Lactobacillus acidophilus Lactobacillus casei Lipoteichoic acids Osmotic stress S-layer proteins Stress condition Biosynthesis envelope protein lipoteichoic acid messenger RNA peptidoglycan probiotic agent protein signal peptide sodium chloride surface layer protein a surface layer protein x unclassified drug membrane protein S-layer proteins adaptation bacterium gene expression genetic analysis hypersaline environment osmosis physiological response protein RNA adaptation Article cell wall controlled study gene expression profiling genetic regulation genome analysis growth curve human Lactobacillus acidophilus Lactobacillus casei osmotic stress protein modification protein synthesis salt stress surface property drug effects gene expression genetics Lactobacillus acidophilus metabolism osmotic pressure Lactobacillus Lactobacillus acidophilus Lactobacillus casei Gene Expression Lactobacillus acidophilus Membrane Glycoproteins Osmotic Pressure Sodium Chloride Palomino, M.M. Waehner, P.M. Fina Martin, J. Ojeda, P. Malone, L. Sánchez Rivas, C. Prado Acosta, M. Allievi, M.C. Ruzal, S.M. Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356
topic_facet	Lactobacillus Osmotic stress S-layer proteins Bacilli Gene expression Osmosis Proteins Sodium chloride Cell-wall thickness Lactobacillus Lactobacillus acidophilus Lactobacillus casei Lipoteichoic acids Osmotic stress S-layer proteins Stress condition Biosynthesis envelope protein lipoteichoic acid messenger RNA peptidoglycan probiotic agent protein signal peptide sodium chloride surface layer protein a surface layer protein x unclassified drug membrane protein S-layer proteins adaptation bacterium gene expression genetic analysis hypersaline environment osmosis physiological response protein RNA adaptation Article cell wall controlled study gene expression profiling genetic regulation genome analysis growth curve human Lactobacillus acidophilus Lactobacillus casei osmotic stress protein modification protein synthesis salt stress surface property drug effects gene expression genetics Lactobacillus acidophilus metabolism osmotic pressure Lactobacillus Lactobacillus acidophilus Lactobacillus casei Gene Expression Lactobacillus acidophilus Membrane Glycoproteins Osmotic Pressure Sodium Chloride
description	In this work, we studied the role of surface layer (S-layer) proteins in the adaptation of Lactobacillus acidophilus ATCC 4356 to the osmotic stress generated by high salt. The amounts of the predominant and the auxiliary S-layer proteins SlpA and SlpX were strongly influenced by the growth phase and high-salt conditions (0.6 M NaCl). Changes in gene expression were also observed as the mRNAs of the slpA and slpX genes increased related to the growth phase and presence of high salt. A growth stage-dependent modification on the S-layer protein profile in response to NaCl was observed: while in control conditions, the auxiliary SlpX protein represented less than 10 % of the total S-layer protein, in high-salt conditions, it increased to almost 40 % in the stationary phase. The increase in S-layer protein synthesis in the stress condition could be a consequence of or a way to counteract the fragility of the cell wall, since a decrease in the cell wall thickness and envelope components (peptidoglycan layer and lipoteichoic acid content) was observed in L. acidophilus when compared to a non-S-layer-producing species such as Lactobacillus casei. Also, the stationary phase and growth in high-salt medium resulted in increased release of S-layer proteins to the supernatant medium. Overall, these findings suggest that pre-growth in high-salt conditions would result in an advantage for the probiotic nature of L. acidophilus ATCC 4356 as the increased amount and release of the S-layer might be appropriate for its antimicrobial capacity. © 2016, Springer-Verlag Berlin Heidelberg.
format	JOUR
author	Palomino, M.M. Waehner, P.M. Fina Martin, J. Ojeda, P. Malone, L. Sánchez Rivas, C. Prado Acosta, M. Allievi, M.C. Ruzal, S.M.
author_facet	Palomino, M.M. Waehner, P.M. Fina Martin, J. Ojeda, P. Malone, L. Sánchez Rivas, C. Prado Acosta, M. Allievi, M.C. Ruzal, S.M.
author_sort	Palomino, M.M.
title	Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356
title_short	Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356
title_full	Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356
title_fullStr	Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356
title_full_unstemmed	Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356
title_sort	influence of osmotic stress on the profile and gene expression of surface layer proteins in lactobacillus acidophilus atcc 4356
url	http://hdl.handle.net/20.500.12110/paper_01757598_v100_n19_p8475_Palomino
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Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356

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