Cholesterol modulation of nicotinic acetylcholine receptor surface mobility
Nicotinic acetylcholine receptor (AChR) function and distribution are quite sensitive to cholesterol (Chol) levels in the plasma membrane (reviewed by Barrantes in J Neurochem 103 (suppl 1):72-80, 2007). Here we combined confocal fluorescence recovery after photobleaching (FRAP) and confocal fluores...
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todo:paper_01757571_v39_n2_p213_Baier2023-10-03T15:07:51Z Cholesterol modulation of nicotinic acetylcholine receptor surface mobility Baier, C.J. Gallegos, C.E. Levi, V. Barrantes, F.J. Acetylcholine receptor Cholesterol Lateral mobility Lipid microdomains Membrane Nicotinic Actins Animals beta-Cyclodextrins Bicyclo Compounds, Heterocyclic Cell Membrane CHO Cells Cholesterol Cricetinae Cricetulus Diffusion Fluorescence Recovery After Photobleaching Microscopy, Confocal Motion Protein Multimerization Receptors, Nicotinic Spectrometry, Fluorescence Thiazolidines Mammalia Nicotinic acetylcholine receptor (AChR) function and distribution are quite sensitive to cholesterol (Chol) levels in the plasma membrane (reviewed by Barrantes in J Neurochem 103 (suppl 1):72-80, 2007). Here we combined confocal fluorescence recovery after photobleaching (FRAP) and confocal fluorescence correlation spectroscopy (FCS) to examine the mobility of the AChR and its dependence on Chol content at the cell surface of a mammalian cell line. Plasma membrane AChR exhibited limited mobility and only ~55% of the fluorescence was recovered within 10 min after photobleaching. Depletion of membrane Chol by methyl-β-cyclodextrin strongly affected the mobility of the AChR at the plasma membrane; the fraction of mobile AChR fell from 55 to 20% in Chol-depleted cells, whereas Chol enrichment by methyl-β-cyclodextrin-Chol treatment did not reduce receptor mobility at the cell surface. Actin depolymerization caused by latrunculin A partially restored receptor mobility in Chol-depleted cells. In agreement with the FRAP data, scanning FCS experiments showed that the diffusion coefficient of the AChR was about 30% lower upon Chol depletion. Taken together, these results suggest that membrane Chol modulates AChR mobility at the plasma membrane through a Chol-dependent mechanism sensitive to cortical actin. © 2009 European Biophysical Societies' Association. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01757571_v39_n2_p213_Baier |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acetylcholine receptor Cholesterol Lateral mobility Lipid microdomains Membrane Nicotinic Actins Animals beta-Cyclodextrins Bicyclo Compounds, Heterocyclic Cell Membrane CHO Cells Cholesterol Cricetinae Cricetulus Diffusion Fluorescence Recovery After Photobleaching Microscopy, Confocal Motion Protein Multimerization Receptors, Nicotinic Spectrometry, Fluorescence Thiazolidines Mammalia |
| spellingShingle |
Acetylcholine receptor Cholesterol Lateral mobility Lipid microdomains Membrane Nicotinic Actins Animals beta-Cyclodextrins Bicyclo Compounds, Heterocyclic Cell Membrane CHO Cells Cholesterol Cricetinae Cricetulus Diffusion Fluorescence Recovery After Photobleaching Microscopy, Confocal Motion Protein Multimerization Receptors, Nicotinic Spectrometry, Fluorescence Thiazolidines Mammalia Baier, C.J. Gallegos, C.E. Levi, V. Barrantes, F.J. Cholesterol modulation of nicotinic acetylcholine receptor surface mobility |
| topic_facet |
Acetylcholine receptor Cholesterol Lateral mobility Lipid microdomains Membrane Nicotinic Actins Animals beta-Cyclodextrins Bicyclo Compounds, Heterocyclic Cell Membrane CHO Cells Cholesterol Cricetinae Cricetulus Diffusion Fluorescence Recovery After Photobleaching Microscopy, Confocal Motion Protein Multimerization Receptors, Nicotinic Spectrometry, Fluorescence Thiazolidines Mammalia |
| description |
Nicotinic acetylcholine receptor (AChR) function and distribution are quite sensitive to cholesterol (Chol) levels in the plasma membrane (reviewed by Barrantes in J Neurochem 103 (suppl 1):72-80, 2007). Here we combined confocal fluorescence recovery after photobleaching (FRAP) and confocal fluorescence correlation spectroscopy (FCS) to examine the mobility of the AChR and its dependence on Chol content at the cell surface of a mammalian cell line. Plasma membrane AChR exhibited limited mobility and only ~55% of the fluorescence was recovered within 10 min after photobleaching. Depletion of membrane Chol by methyl-β-cyclodextrin strongly affected the mobility of the AChR at the plasma membrane; the fraction of mobile AChR fell from 55 to 20% in Chol-depleted cells, whereas Chol enrichment by methyl-β-cyclodextrin-Chol treatment did not reduce receptor mobility at the cell surface. Actin depolymerization caused by latrunculin A partially restored receptor mobility in Chol-depleted cells. In agreement with the FRAP data, scanning FCS experiments showed that the diffusion coefficient of the AChR was about 30% lower upon Chol depletion. Taken together, these results suggest that membrane Chol modulates AChR mobility at the plasma membrane through a Chol-dependent mechanism sensitive to cortical actin. © 2009 European Biophysical Societies' Association. |
| format |
JOUR |
| author |
Baier, C.J. Gallegos, C.E. Levi, V. Barrantes, F.J. |
| author_facet |
Baier, C.J. Gallegos, C.E. Levi, V. Barrantes, F.J. |
| author_sort |
Baier, C.J. |
| title |
Cholesterol modulation of nicotinic acetylcholine receptor surface mobility |
| title_short |
Cholesterol modulation of nicotinic acetylcholine receptor surface mobility |
| title_full |
Cholesterol modulation of nicotinic acetylcholine receptor surface mobility |
| title_fullStr |
Cholesterol modulation of nicotinic acetylcholine receptor surface mobility |
| title_full_unstemmed |
Cholesterol modulation of nicotinic acetylcholine receptor surface mobility |
| title_sort |
cholesterol modulation of nicotinic acetylcholine receptor surface mobility |
| url |
http://hdl.handle.net/20.500.12110/paper_01757571_v39_n2_p213_Baier |
| work_keys_str_mv |
AT baiercj cholesterolmodulationofnicotinicacetylcholinereceptorsurfacemobility AT gallegosce cholesterolmodulationofnicotinicacetylcholinereceptorsurfacemobility AT leviv cholesterolmodulationofnicotinicacetylcholinereceptorsurfacemobility AT barrantesfj cholesterolmodulationofnicotinicacetylcholinereceptorsurfacemobility |
| _version_ |
1807318817378926592 |