Pregnenolone and progesterone metabolism by the testes of Bufo arenarum

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Sliced testis tissue from Bufo arenarum was incubated in the presence of [3H]pregnenolone. Testis fragments were also used for double isotope experiments using [3H]pregnenolone and [14C]progesterone. Specific activities were equated with the addition of radioinert pregnenolone. When yields of radiom...

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Autores principales: Canosa, L.F., Pozzi, A.G., Ceballos, N.R.
Formato: JOUR
Materias:
Anura
Steroid hormones
Steroidogenesis
Testes
Toad
Bufo arenarum
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01741578_v168_n7_p491_Canosa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01741578_v168_n7_p491_Canosa
record_format dspace
spelling todo:paper_01741578_v168_n7_p491_Canosa2023-10-03T15:07:47Z Pregnenolone and progesterone metabolism by the testes of Bufo arenarum Canosa, L.F. Pozzi, A.G. Ceballos, N.R. Anura Steroid hormones Steroidogenesis Testes Toad Bufo arenarum Sliced testis tissue from Bufo arenarum was incubated in the presence of [3H]pregnenolone. Testis fragments were also used for double isotope experiments using [3H]pregnenolone and [14C]progesterone. Specific activities were equated with the addition of radioinert pregnenolone. When yields of radiometabolites were analysed, pregnenolone was found to be a good precursor for C19 steroids such as dehydroepiandrosterone, 5-androsten-3β,17β diol, testosterone, 5α-dihydrotestosterone and a C21 steroid, 5α-pregnan-3,20 dione. Progesterone mainly converts to 5α-pregnan-3,20 dione, asteroid with unknown function in amphibians. The 5-ene pathway, including 5-androsten-3β,17β diol as intermediate, could be predominant for androgen biosynthesis. Testes bypass not only progesterone but also androstenedione for testosterone biosynthesis. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01741578_v168_n7_p491_Canosa
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Anura
Steroid hormones
Steroidogenesis
Testes
Toad
Bufo arenarum
spellingShingle Anura
Steroid hormones
Steroidogenesis
Testes
Toad
Bufo arenarum
Canosa, L.F.
Pozzi, A.G.
Ceballos, N.R.
Pregnenolone and progesterone metabolism by the testes of Bufo arenarum
topic_facet Anura
Steroid hormones
Steroidogenesis
Testes
Toad
Bufo arenarum
description Sliced testis tissue from Bufo arenarum was incubated in the presence of [3H]pregnenolone. Testis fragments were also used for double isotope experiments using [3H]pregnenolone and [14C]progesterone. Specific activities were equated with the addition of radioinert pregnenolone. When yields of radiometabolites were analysed, pregnenolone was found to be a good precursor for C19 steroids such as dehydroepiandrosterone, 5-androsten-3β,17β diol, testosterone, 5α-dihydrotestosterone and a C21 steroid, 5α-pregnan-3,20 dione. Progesterone mainly converts to 5α-pregnan-3,20 dione, asteroid with unknown function in amphibians. The 5-ene pathway, including 5-androsten-3β,17β diol as intermediate, could be predominant for androgen biosynthesis. Testes bypass not only progesterone but also androstenedione for testosterone biosynthesis.
format JOUR
author Canosa, L.F.
Pozzi, A.G.
Ceballos, N.R.
author_facet Canosa, L.F.
Pozzi, A.G.
Ceballos, N.R.
author_sort Canosa, L.F.
title Pregnenolone and progesterone metabolism by the testes of Bufo arenarum
title_short Pregnenolone and progesterone metabolism by the testes of Bufo arenarum
title_full Pregnenolone and progesterone metabolism by the testes of Bufo arenarum
title_fullStr Pregnenolone and progesterone metabolism by the testes of Bufo arenarum
title_full_unstemmed Pregnenolone and progesterone metabolism by the testes of Bufo arenarum
title_sort pregnenolone and progesterone metabolism by the testes of bufo arenarum
url http://hdl.handle.net/20.500.12110/paper_01741578_v168_n7_p491_Canosa
work_keys_str_mv AT canosalf pregnenoloneandprogesteronemetabolismbythetestesofbufoarenarum
AT pozziag pregnenoloneandprogesteronemetabolismbythetestesofbufoarenarum
AT ceballosnr pregnenoloneandprogesteronemetabolismbythetestesofbufoarenarum
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