Expression of heme oxygenase-1 in non-small cell lung cancer (NSCLC) and its correlation with clinical data
While changes in heme oxygenase (HO-1) in lung cancer have already been reported, conflicting results were obtained for enzyme expression in human lung cancer specimens. Therefore, the aim of this work was to study HO-1 expression in a large collection of human lung cancer samples. For this purpose,...
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todo:paper_01695002_v77_n1_p168_Degese2023-10-03T15:07:11Z Expression of heme oxygenase-1 in non-small cell lung cancer (NSCLC) and its correlation with clinical data Degese, M.S. Mendizabal, J.E. Gandini, N.A. Gutkind, J.S. Molinolo, A. Hewitt, S.M. Curino, A.C. Coso, O.A. Facchinetti, M.M. Heme-oxygenase-1 Immunohistochemistry Lung cancer NSCLC Tissue microarray heme oxygenase 1 adult article cancer staging female histopathology human human cell human tissue immunohistochemistry lung carcinoma lung non small cell cancer lymph node major clinical study male priority journal protein expression protein function real time polymerase chain reaction tissue microarray upregulation Animals Carcinoma, Non-Small-Cell Lung Cell Line, Tumor Epithelial Cells Female Gene Expression Heme Oxygenase-1 Humans Kaplan-Meier Estimate Lung Neoplasms Lymphatic Metastasis Male Mice NIH 3T3 Cells Real-Time Polymerase Chain Reaction Tissue Array Analysis Up-Regulation While changes in heme oxygenase (HO-1) in lung cancer have already been reported, conflicting results were obtained for enzyme expression in human lung cancer specimens. Therefore, the aim of this work was to study HO-1 expression in a large collection of human lung cancer samples. For this purpose, we analyzed the expression of HO-1 in an organized tissue microarray (TMA) and investigated its correlation with clinicopathological data. Ninety-six percent of tumor samples were positive for HO-1, and the expression of HO-1 was significantly higher in cancerous than in non-cancerous tissues. Importantly, HO-1 expression correlated with advanced stages and lymph node involvement. Additionally, quantitative RT-PCR in 18 pairs of human lung carcinomas and their adjacent non-malignant tissues was performed. Our results demonstrate that HO-1 protein is upregulated in epithelial malignant cells in NSCLC and its expression is associated with higher stages of the disease. Additionally, different subcellular localization is observed between tumor and adjacent non-malignant tissues. © 2012 Elsevier Ireland Ltd. Fil:Degese, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01695002_v77_n1_p168_Degese |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Heme-oxygenase-1 Immunohistochemistry Lung cancer NSCLC Tissue microarray heme oxygenase 1 adult article cancer staging female histopathology human human cell human tissue immunohistochemistry lung carcinoma lung non small cell cancer lymph node major clinical study male priority journal protein expression protein function real time polymerase chain reaction tissue microarray upregulation Animals Carcinoma, Non-Small-Cell Lung Cell Line, Tumor Epithelial Cells Female Gene Expression Heme Oxygenase-1 Humans Kaplan-Meier Estimate Lung Neoplasms Lymphatic Metastasis Male Mice NIH 3T3 Cells Real-Time Polymerase Chain Reaction Tissue Array Analysis Up-Regulation |
| spellingShingle |
Heme-oxygenase-1 Immunohistochemistry Lung cancer NSCLC Tissue microarray heme oxygenase 1 adult article cancer staging female histopathology human human cell human tissue immunohistochemistry lung carcinoma lung non small cell cancer lymph node major clinical study male priority journal protein expression protein function real time polymerase chain reaction tissue microarray upregulation Animals Carcinoma, Non-Small-Cell Lung Cell Line, Tumor Epithelial Cells Female Gene Expression Heme Oxygenase-1 Humans Kaplan-Meier Estimate Lung Neoplasms Lymphatic Metastasis Male Mice NIH 3T3 Cells Real-Time Polymerase Chain Reaction Tissue Array Analysis Up-Regulation Degese, M.S. Mendizabal, J.E. Gandini, N.A. Gutkind, J.S. Molinolo, A. Hewitt, S.M. Curino, A.C. Coso, O.A. Facchinetti, M.M. Expression of heme oxygenase-1 in non-small cell lung cancer (NSCLC) and its correlation with clinical data |
| topic_facet |
Heme-oxygenase-1 Immunohistochemistry Lung cancer NSCLC Tissue microarray heme oxygenase 1 adult article cancer staging female histopathology human human cell human tissue immunohistochemistry lung carcinoma lung non small cell cancer lymph node major clinical study male priority journal protein expression protein function real time polymerase chain reaction tissue microarray upregulation Animals Carcinoma, Non-Small-Cell Lung Cell Line, Tumor Epithelial Cells Female Gene Expression Heme Oxygenase-1 Humans Kaplan-Meier Estimate Lung Neoplasms Lymphatic Metastasis Male Mice NIH 3T3 Cells Real-Time Polymerase Chain Reaction Tissue Array Analysis Up-Regulation |
| description |
While changes in heme oxygenase (HO-1) in lung cancer have already been reported, conflicting results were obtained for enzyme expression in human lung cancer specimens. Therefore, the aim of this work was to study HO-1 expression in a large collection of human lung cancer samples. For this purpose, we analyzed the expression of HO-1 in an organized tissue microarray (TMA) and investigated its correlation with clinicopathological data. Ninety-six percent of tumor samples were positive for HO-1, and the expression of HO-1 was significantly higher in cancerous than in non-cancerous tissues. Importantly, HO-1 expression correlated with advanced stages and lymph node involvement. Additionally, quantitative RT-PCR in 18 pairs of human lung carcinomas and their adjacent non-malignant tissues was performed. Our results demonstrate that HO-1 protein is upregulated in epithelial malignant cells in NSCLC and its expression is associated with higher stages of the disease. Additionally, different subcellular localization is observed between tumor and adjacent non-malignant tissues. © 2012 Elsevier Ireland Ltd. |
| format |
JOUR |
| author |
Degese, M.S. Mendizabal, J.E. Gandini, N.A. Gutkind, J.S. Molinolo, A. Hewitt, S.M. Curino, A.C. Coso, O.A. Facchinetti, M.M. |
| author_facet |
Degese, M.S. Mendizabal, J.E. Gandini, N.A. Gutkind, J.S. Molinolo, A. Hewitt, S.M. Curino, A.C. Coso, O.A. Facchinetti, M.M. |
| author_sort |
Degese, M.S. |
| title |
Expression of heme oxygenase-1 in non-small cell lung cancer (NSCLC) and its correlation with clinical data |
| title_short |
Expression of heme oxygenase-1 in non-small cell lung cancer (NSCLC) and its correlation with clinical data |
| title_full |
Expression of heme oxygenase-1 in non-small cell lung cancer (NSCLC) and its correlation with clinical data |
| title_fullStr |
Expression of heme oxygenase-1 in non-small cell lung cancer (NSCLC) and its correlation with clinical data |
| title_full_unstemmed |
Expression of heme oxygenase-1 in non-small cell lung cancer (NSCLC) and its correlation with clinical data |
| title_sort |
expression of heme oxygenase-1 in non-small cell lung cancer (nsclc) and its correlation with clinical data |
| url |
http://hdl.handle.net/20.500.12110/paper_01695002_v77_n1_p168_Degese |
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