Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development
We evaluated the in vitro phosphorylation of the presynaptic substrate of protein kinase C (PKC), GAP-43/B-50 and the PKC activity in the striatum of rats submitted to a circling training (CT) test during postnatal development. Motor activity at 30 days of age, but not at other ages, produced a unil...
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todo:paper_0169328X_v65_n1_p34_Paratcha2023-10-03T15:07:03Z Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development Paratcha, G.Ch. Ibarra, G.R. Wolansky, M.J. Rodriguez, J.A. Azcurra, J.M. Circling training Development GAP-43/B-50 PKC Rat striatum SPM neuromodulin protein kinase c animal experiment animal tissue article circling behavior controlled study corpus striatum enzyme activity enzyme substrate male motor performance nerve cell plasticity nonhuman priority journal protein phosphorylation rat synaptic membrane Animals Behavior, Animal Blotting, Western Cell Membrane Conditioning (Psychology) Corpus Striatum GAP-43 Protein Locomotion Male Motor Neurons Phosphorylation Protein Kinase C Rats Rats, Sprague-Dawley Synapses We evaluated the in vitro phosphorylation of the presynaptic substrate of protein kinase C (PKC), GAP-43/B-50 and the PKC activity in the striatum of rats submitted to a circling training (CT) test during postnatal development. Motor activity at 30 days of age, but not at other ages, produced a unilateral reduction (-29.5%; p < 0.001) in the level of GAP- 43/B-50 endogenous phosphorylation in the contralateral striatum with respect to the ipsilateral side, while non-trained control animals did not show asymmetric differences. Compared to controls, the contralateral striatum of trained animals also showed a significant reduction (-29.3%; p < 0.001) in the incorporation of 32P-phosphate into GAP-43. This decreased in vitro GAP-43 phosphorylation was seen at 30 min, but not immediately after circling motor behavior. This contralateral change in GAP-43 phosphorylation correlated with the running speed developed by the animals [(r = 0.9443, p = 0.0046, n = 6, relative to control group) and (r = 0.8813, p = 0.0203, n = 6, with respect to the ipsilateral side of the exercised animals)]. On the contrary, GAP-43/B-50 immunoblots did not show changes in the amount of this phosphoprotein among the different experiment groups. Back phosphorylation assays, performed in the presence of bovine purified PKC, increased the level of GAP-43/B-50 phosphorylation in the striatum contralateral to the sense of turning [(+22%; p < 0.05, with respect to ipsilateral side of the same trained group) and (+21%; p < 0.05, relative to control group)]. Taken together, these results demonstrate that the activity developed in the CT test induces a reduction in the phosphorylation state of GAP-43/B-50 in the specific site for PKC. We conclude that general markers of activity-dependent neuronal plasticity are also altered in the same period that long-lasting changes in striatal neuroreceptors are triggered by circling motor behavior. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0169328X_v65_n1_p34_Paratcha |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Circling training Development GAP-43/B-50 PKC Rat striatum SPM neuromodulin protein kinase c animal experiment animal tissue article circling behavior controlled study corpus striatum enzyme activity enzyme substrate male motor performance nerve cell plasticity nonhuman priority journal protein phosphorylation rat synaptic membrane Animals Behavior, Animal Blotting, Western Cell Membrane Conditioning (Psychology) Corpus Striatum GAP-43 Protein Locomotion Male Motor Neurons Phosphorylation Protein Kinase C Rats Rats, Sprague-Dawley Synapses |
| spellingShingle |
Circling training Development GAP-43/B-50 PKC Rat striatum SPM neuromodulin protein kinase c animal experiment animal tissue article circling behavior controlled study corpus striatum enzyme activity enzyme substrate male motor performance nerve cell plasticity nonhuman priority journal protein phosphorylation rat synaptic membrane Animals Behavior, Animal Blotting, Western Cell Membrane Conditioning (Psychology) Corpus Striatum GAP-43 Protein Locomotion Male Motor Neurons Phosphorylation Protein Kinase C Rats Rats, Sprague-Dawley Synapses Paratcha, G.Ch. Ibarra, G.R. Wolansky, M.J. Rodriguez, J.A. Azcurra, J.M. Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development |
| topic_facet |
Circling training Development GAP-43/B-50 PKC Rat striatum SPM neuromodulin protein kinase c animal experiment animal tissue article circling behavior controlled study corpus striatum enzyme activity enzyme substrate male motor performance nerve cell plasticity nonhuman priority journal protein phosphorylation rat synaptic membrane Animals Behavior, Animal Blotting, Western Cell Membrane Conditioning (Psychology) Corpus Striatum GAP-43 Protein Locomotion Male Motor Neurons Phosphorylation Protein Kinase C Rats Rats, Sprague-Dawley Synapses |
| description |
We evaluated the in vitro phosphorylation of the presynaptic substrate of protein kinase C (PKC), GAP-43/B-50 and the PKC activity in the striatum of rats submitted to a circling training (CT) test during postnatal development. Motor activity at 30 days of age, but not at other ages, produced a unilateral reduction (-29.5%; p < 0.001) in the level of GAP- 43/B-50 endogenous phosphorylation in the contralateral striatum with respect to the ipsilateral side, while non-trained control animals did not show asymmetric differences. Compared to controls, the contralateral striatum of trained animals also showed a significant reduction (-29.3%; p < 0.001) in the incorporation of 32P-phosphate into GAP-43. This decreased in vitro GAP-43 phosphorylation was seen at 30 min, but not immediately after circling motor behavior. This contralateral change in GAP-43 phosphorylation correlated with the running speed developed by the animals [(r = 0.9443, p = 0.0046, n = 6, relative to control group) and (r = 0.8813, p = 0.0203, n = 6, with respect to the ipsilateral side of the exercised animals)]. On the contrary, GAP-43/B-50 immunoblots did not show changes in the amount of this phosphoprotein among the different experiment groups. Back phosphorylation assays, performed in the presence of bovine purified PKC, increased the level of GAP-43/B-50 phosphorylation in the striatum contralateral to the sense of turning [(+22%; p < 0.05, with respect to ipsilateral side of the same trained group) and (+21%; p < 0.05, relative to control group)]. Taken together, these results demonstrate that the activity developed in the CT test induces a reduction in the phosphorylation state of GAP-43/B-50 in the specific site for PKC. We conclude that general markers of activity-dependent neuronal plasticity are also altered in the same period that long-lasting changes in striatal neuroreceptors are triggered by circling motor behavior. |
| format |
JOUR |
| author |
Paratcha, G.Ch. Ibarra, G.R. Wolansky, M.J. Rodriguez, J.A. Azcurra, J.M. |
| author_facet |
Paratcha, G.Ch. Ibarra, G.R. Wolansky, M.J. Rodriguez, J.A. Azcurra, J.M. |
| author_sort |
Paratcha, G.Ch. |
| title |
Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development |
| title_short |
Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development |
| title_full |
Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development |
| title_fullStr |
Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development |
| title_full_unstemmed |
Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development |
| title_sort |
decreased gap-43/b-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development |
| url |
http://hdl.handle.net/20.500.12110/paper_0169328X_v65_n1_p34_Paratcha |
| work_keys_str_mv |
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| _version_ |
1807316912323952640 |