UDP-glucose:protein transglucosylase in developing maize endosperm

UDP-Glc:protein transglucosylase (UPTG) (EC 2.4.1.112), involved in the first step of a two-step mechanism proposed in potato tuber for protein-bound α-glucan synthesis, was found in developing maize endosperm. The enzyme transfers glucosyl residues from UDP-[14C]glucose to an endogenous protein acc...

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Detalles Bibliográficos
Autores principales: Rothschild, A., Tandecarz, J.S.
Formato: JOUR
Materias:
Developing maize endosperm
Protein acceptor
protein transglucosylase
UDP-Glc
Zea mays
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01689452_v97_n2_p119_Rothschild
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_01689452_v97_n2_p119_Rothschild2023-10-03T15:06:53Z UDP-glucose:protein transglucosylase in developing maize endosperm Rothschild, A. Tandecarz, J.S. Developing maize endosperm Protein acceptor protein transglucosylase UDP-Glc Zea mays Zea mays UDP-Glc:protein transglucosylase (UPTG) (EC 2.4.1.112), involved in the first step of a two-step mechanism proposed in potato tuber for protein-bound α-glucan synthesis, was found in developing maize endosperm. The enzyme transfers glucosyl residues from UDP-[14C]glucose to an endogenous protein acceptor in a Mn2+-dependent reaction in the absence of added primer. After SDS-polycrylamide gel electrophrresis of the reaction product, radioactivity co-migrated with a 38-kDa protein band. A two-step experiment shows that the developing endosperm of maize contains phosphorylase and starch synthase, capable of utilizing the UPTG reaction product formed during the first step as a primer for the synthesis of protein-bound glucan in the presence of the respective glucosyl donors during the second incubation step. © 1994. Fil:Rothschild, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tandecarz, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01689452_v97_n2_p119_Rothschild
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Developing maize endosperm
Protein acceptor
protein transglucosylase
UDP-Glc
Zea mays
Zea mays
spellingShingle Developing maize endosperm
Protein acceptor
protein transglucosylase
UDP-Glc
Zea mays
Zea mays
Rothschild, A.
Tandecarz, J.S.
UDP-glucose:protein transglucosylase in developing maize endosperm
topic_facet Developing maize endosperm
Protein acceptor
protein transglucosylase
UDP-Glc
Zea mays
Zea mays
description UDP-Glc:protein transglucosylase (UPTG) (EC 2.4.1.112), involved in the first step of a two-step mechanism proposed in potato tuber for protein-bound α-glucan synthesis, was found in developing maize endosperm. The enzyme transfers glucosyl residues from UDP-[14C]glucose to an endogenous protein acceptor in a Mn2+-dependent reaction in the absence of added primer. After SDS-polycrylamide gel electrophrresis of the reaction product, radioactivity co-migrated with a 38-kDa protein band. A two-step experiment shows that the developing endosperm of maize contains phosphorylase and starch synthase, capable of utilizing the UPTG reaction product formed during the first step as a primer for the synthesis of protein-bound glucan in the presence of the respective glucosyl donors during the second incubation step. © 1994.
format JOUR
author Rothschild, A.
Tandecarz, J.S.
author_facet Rothschild, A.
Tandecarz, J.S.
author_sort Rothschild, A.
title UDP-glucose:protein transglucosylase in developing maize endosperm
title_short UDP-glucose:protein transglucosylase in developing maize endosperm
title_full UDP-glucose:protein transglucosylase in developing maize endosperm
title_fullStr UDP-glucose:protein transglucosylase in developing maize endosperm
title_full_unstemmed UDP-glucose:protein transglucosylase in developing maize endosperm
title_sort udp-glucose:protein transglucosylase in developing maize endosperm
url http://hdl.handle.net/20.500.12110/paper_01689452_v97_n2_p119_Rothschild
work_keys_str_mv AT rothschilda udpglucoseproteintransglucosylaseindevelopingmaizeendosperm
AT tandecarzjs udpglucoseproteintransglucosylaseindevelopingmaizeendosperm
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