Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators
The cell wall composition of apples callus cultures showed changes in the presence of 5mgl-1 of three different plant growth regulators (PGRs), namely picloram, abscisic acid and gibberellic acid. Although the structural functions of cell walls do not generally allow for pronounced variations of the...
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todo:paper_01689452_v185-186_n_p169_AlayonLuaces2023-10-03T15:06:51Z Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators Alayón-Luaces, P. Ponce, N.M.A. Mroginski, L.A. Stortz, C.A. Sozzi, G.O. Callus cultures Cell wall Cross-linking glycan Malus domestica Pectin Plant growth regulators abscisic acid arabinose gibberellic acid gibberellin monosaccharide pectin phytohormone picloram polysaccharide uronic acid article cell wall comparative study culture technique drug effect fruit Malus metabolism Abscisic Acid Arabinose Cell Culture Techniques Cell Wall Fruit Gibberellins Malus Monosaccharides Pectins Picloram Plant Growth Regulators Polysaccharides Uronic Acids Malus x domestica The cell wall composition of apples callus cultures showed changes in the presence of 5mgl-1 of three different plant growth regulators (PGRs), namely picloram, abscisic acid and gibberellic acid. Although the structural functions of cell walls do not generally allow for pronounced variations of the total pectin and matrix glycan content, this work provides evidence that the addition of these plant growth regulators can rule, at least partly, cell wall metabolism in apple callus cultures. The chelator- and carbonate-extracts always had the analytical characteristics of pectins, with high proportions of uronic acids, arabinose and galactose as the main monosaccharides, and a significant proportion of rhamnose, but the cross-linking glycan fractions were still rich in RG-I-like material. The application of PGRs produced shifts of uronic acid and neutral sugars between fractions. Arabinose was the neutral sugar exhibiting more variations in apple callus cell wall. Picloram and abscisic acid produced an increase of the uronic acid contents of the cell walls. The AIRs obtained from calluses treated with different PGRs did not show large amounts of high molecular weight products, as determined by size-exclusion chromatography. For the carbonate-extract only the callus treated with picloram displayed two separated peaks for products of different molecular weights. The chromatographic profiles for the 4% KOH-extract displayed two peaks for all the treatments, one very sharp with high molecular weight, and another one wider of smaller molecular weight, whereas the difference between treatments can only be appraised through the areas of the peaks. This is the first report on cell wall composition from fruit calluses supplemented with different PGRs. © 2011 Elsevier Ireland Ltd. Fil:Stortz, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sozzi, G.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01689452_v185-186_n_p169_AlayonLuaces |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Callus cultures Cell wall Cross-linking glycan Malus domestica Pectin Plant growth regulators abscisic acid arabinose gibberellic acid gibberellin monosaccharide pectin phytohormone picloram polysaccharide uronic acid article cell wall comparative study culture technique drug effect fruit Malus metabolism Abscisic Acid Arabinose Cell Culture Techniques Cell Wall Fruit Gibberellins Malus Monosaccharides Pectins Picloram Plant Growth Regulators Polysaccharides Uronic Acids Malus x domestica |
| spellingShingle |
Callus cultures Cell wall Cross-linking glycan Malus domestica Pectin Plant growth regulators abscisic acid arabinose gibberellic acid gibberellin monosaccharide pectin phytohormone picloram polysaccharide uronic acid article cell wall comparative study culture technique drug effect fruit Malus metabolism Abscisic Acid Arabinose Cell Culture Techniques Cell Wall Fruit Gibberellins Malus Monosaccharides Pectins Picloram Plant Growth Regulators Polysaccharides Uronic Acids Malus x domestica Alayón-Luaces, P. Ponce, N.M.A. Mroginski, L.A. Stortz, C.A. Sozzi, G.O. Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators |
| topic_facet |
Callus cultures Cell wall Cross-linking glycan Malus domestica Pectin Plant growth regulators abscisic acid arabinose gibberellic acid gibberellin monosaccharide pectin phytohormone picloram polysaccharide uronic acid article cell wall comparative study culture technique drug effect fruit Malus metabolism Abscisic Acid Arabinose Cell Culture Techniques Cell Wall Fruit Gibberellins Malus Monosaccharides Pectins Picloram Plant Growth Regulators Polysaccharides Uronic Acids Malus x domestica |
| description |
The cell wall composition of apples callus cultures showed changes in the presence of 5mgl-1 of three different plant growth regulators (PGRs), namely picloram, abscisic acid and gibberellic acid. Although the structural functions of cell walls do not generally allow for pronounced variations of the total pectin and matrix glycan content, this work provides evidence that the addition of these plant growth regulators can rule, at least partly, cell wall metabolism in apple callus cultures. The chelator- and carbonate-extracts always had the analytical characteristics of pectins, with high proportions of uronic acids, arabinose and galactose as the main monosaccharides, and a significant proportion of rhamnose, but the cross-linking glycan fractions were still rich in RG-I-like material. The application of PGRs produced shifts of uronic acid and neutral sugars between fractions. Arabinose was the neutral sugar exhibiting more variations in apple callus cell wall. Picloram and abscisic acid produced an increase of the uronic acid contents of the cell walls. The AIRs obtained from calluses treated with different PGRs did not show large amounts of high molecular weight products, as determined by size-exclusion chromatography. For the carbonate-extract only the callus treated with picloram displayed two separated peaks for products of different molecular weights. The chromatographic profiles for the 4% KOH-extract displayed two peaks for all the treatments, one very sharp with high molecular weight, and another one wider of smaller molecular weight, whereas the difference between treatments can only be appraised through the areas of the peaks. This is the first report on cell wall composition from fruit calluses supplemented with different PGRs. © 2011 Elsevier Ireland Ltd. |
| format |
JOUR |
| author |
Alayón-Luaces, P. Ponce, N.M.A. Mroginski, L.A. Stortz, C.A. Sozzi, G.O. |
| author_facet |
Alayón-Luaces, P. Ponce, N.M.A. Mroginski, L.A. Stortz, C.A. Sozzi, G.O. |
| author_sort |
Alayón-Luaces, P. |
| title |
Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators |
| title_short |
Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators |
| title_full |
Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators |
| title_fullStr |
Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators |
| title_full_unstemmed |
Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators |
| title_sort |
compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators |
| url |
http://hdl.handle.net/20.500.12110/paper_01689452_v185-186_n_p169_AlayonLuaces |
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