Intermediate filament integrity is required for Junin virus replication

The role of the cytoskeletal framework in Junin virus (JUNV) replication has already been demonstrated with compounds interfering with the microfilament (MF) and microtubule (MT) networks. In this work, we evaluated the role of intermediate filaments (IF) during JUNV infection. We tested the effect...

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Autores principales:	Cordo, S.M., Candurra, N.A.
Formato:	JOUR
Materias:	Acrylamide Intermediate filaments Junin virus acrylamide vimentin adsorption article astrocyte cell culture cell differentiation cell type cell viability concentration response controlled study fibroblast immunofluorescence intermediate filament nerve cell nonhuman prepuce priority journal Vero cell virus infection virus replication Murinae
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01681702_v97_n1_p47_Cordo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01681702_v97_n1_p47_Cordo
record_format	dspace
spelling	todo:paper_01681702_v97_n1_p47_Cordo2023-10-03T15:06:18Z Intermediate filament integrity is required for Junin virus replication Cordo, S.M. Candurra, N.A. Acrylamide Intermediate filaments Junin virus acrylamide vimentin adsorption article astrocyte cell culture cell differentiation cell type cell viability concentration response controlled study fibroblast immunofluorescence intermediate filament Junin virus nerve cell nonhuman prepuce priority journal Vero cell virus infection virus replication Junin virus Murinae The role of the cytoskeletal framework in Junin virus (JUNV) replication has already been demonstrated with compounds interfering with the microfilament (MF) and microtubule (MT) networks. In this work, we evaluated the role of intermediate filaments (IF) during JUNV infection. We tested the effect of acrylamide, a compound that selectively disrupts IF, in culture of three different cell types: Vero cells, murine astrocytes and human foreskin fibroblasts. Perturbation of intermediate filaments had an inhibitory effect on JUNV production within a range of acrylamide concentration of 0.5-3mM in a dose-dependent manner, without cell viability modification. Recovery experiments showed that viral production was partially increased when medium containing acrylamide was replaced by normal maintenance medium (MM). The adsorption and internalization steps were not affected by IF disruption. The expression of JUNV proteins was highly reduced in the presence of 2mM acrylamide while immunofluorescence staining of IF showed network disruption with the formation of cytoplasmic aggregates containing vimentin or glial fibrillary acidic protein (GFAP). We conclude that the IF network may play a role in the early step of JUNV multiplication, subsequent to virus entry and that its integrity is a necessary condition for the normal replication of JUNV in neural and fibroblast cells as well as in the Vero cell line. © 2003 Published by Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681702_v97_n1_p47_Cordo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Acrylamide Intermediate filaments Junin virus acrylamide vimentin adsorption article astrocyte cell culture cell differentiation cell type cell viability concentration response controlled study fibroblast immunofluorescence intermediate filament Junin virus nerve cell nonhuman prepuce priority journal Vero cell virus infection virus replication Junin virus Murinae
spellingShingle	Acrylamide Intermediate filaments Junin virus acrylamide vimentin adsorption article astrocyte cell culture cell differentiation cell type cell viability concentration response controlled study fibroblast immunofluorescence intermediate filament Junin virus nerve cell nonhuman prepuce priority journal Vero cell virus infection virus replication Junin virus Murinae Cordo, S.M. Candurra, N.A. Intermediate filament integrity is required for Junin virus replication
topic_facet	Acrylamide Intermediate filaments Junin virus acrylamide vimentin adsorption article astrocyte cell culture cell differentiation cell type cell viability concentration response controlled study fibroblast immunofluorescence intermediate filament Junin virus nerve cell nonhuman prepuce priority journal Vero cell virus infection virus replication Junin virus Murinae
description	The role of the cytoskeletal framework in Junin virus (JUNV) replication has already been demonstrated with compounds interfering with the microfilament (MF) and microtubule (MT) networks. In this work, we evaluated the role of intermediate filaments (IF) during JUNV infection. We tested the effect of acrylamide, a compound that selectively disrupts IF, in culture of three different cell types: Vero cells, murine astrocytes and human foreskin fibroblasts. Perturbation of intermediate filaments had an inhibitory effect on JUNV production within a range of acrylamide concentration of 0.5-3mM in a dose-dependent manner, without cell viability modification. Recovery experiments showed that viral production was partially increased when medium containing acrylamide was replaced by normal maintenance medium (MM). The adsorption and internalization steps were not affected by IF disruption. The expression of JUNV proteins was highly reduced in the presence of 2mM acrylamide while immunofluorescence staining of IF showed network disruption with the formation of cytoplasmic aggregates containing vimentin or glial fibrillary acidic protein (GFAP). We conclude that the IF network may play a role in the early step of JUNV multiplication, subsequent to virus entry and that its integrity is a necessary condition for the normal replication of JUNV in neural and fibroblast cells as well as in the Vero cell line. © 2003 Published by Elsevier B.V.
format	JOUR
author	Cordo, S.M. Candurra, N.A.
author_facet	Cordo, S.M. Candurra, N.A.
author_sort	Cordo, S.M.
title	Intermediate filament integrity is required for Junin virus replication
title_short	Intermediate filament integrity is required for Junin virus replication
title_full	Intermediate filament integrity is required for Junin virus replication
title_fullStr	Intermediate filament integrity is required for Junin virus replication
title_full_unstemmed	Intermediate filament integrity is required for Junin virus replication
title_sort	intermediate filament integrity is required for junin virus replication
url	http://hdl.handle.net/20.500.12110/paper_01681702_v97_n1_p47_Cordo
work_keys_str_mv	AT cordosm intermediatefilamentintegrityisrequiredforjuninvirusreplication AT candurrana intermediatefilamentintegrityisrequiredforjuninvirusreplication
_version_	1782024254164828160

Intermediate filament integrity is required for Junin virus replication

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