Sequence and phylogenetic analysis of genome segments S1, S2, S3 and S6 of Mal de Río Cuarto virus, a newly accepted Fijivirus species
Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. The nucleotide sequence of four MRCV genome segments was determined. MRCV S1, S2, S3 and S6 were predicted to encode proteins of 168.4, 134.4, 141.7 and 90 kDa, respectively. MRCV S1 encodes a basi...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | JOUR |
| Lenguaje: | English |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01681702_v92_n1_p113_Distefano |
| Aporte de: |
| id |
todo:paper_01681702_v92_n1_p113_Distefano |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_01681702_v92_n1_p113_Distefano2023-10-03T15:06:18Z Sequence and phylogenetic analysis of genome segments S1, S2, S3 and S6 of Mal de Río Cuarto virus, a newly accepted Fijivirus species Distéfano, A.J. Conci, L.R. Muñoz Hidalgo, M. Guzmán, F.A. Hopp, H.E. Del Vas, M. Core protein Fijivirus MRCV Reoviridae RNA-dependent RNA polymerase core protein RNA directed RNA polymerase virus protein virus RNA amino acid sequence article controlled study figi disease virus gene sequence genetic conservation genomics mal de rio cuarto virus nilaparvata lugens reovirus nonhuman nucleotide sequence phylogeny priority journal protein function protein motif Reovirus rice black streaked dwarf virus RNA virus sequence analysis sequence homology virus genome virus identification Amino Acid Sequence Base Sequence Cloning, Molecular Conserved Sequence DNA, Viral Genome, Viral Molecular Sequence Data Phylogeny Reoviridae Sequence Homology, Amino Acid Species Specificity Viral Proteins Fiji disease virus Fijivirus Mal de Rio Cuarto virus Nilaparvata lugens Nilaparvata lugens reovirus Reoviridae Reovirus sp. Rice black streaked dwarf virus Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. The nucleotide sequence of four MRCV genome segments was determined. MRCV S1, S2, S3 and S6 were predicted to encode proteins of 168.4, 134.4, 141.7 and 90 kDa, respectively. MRCV S1 encodes a basic protein that contains conserved RNA-dependent RNA polymerase motifs, and is homologous to Rice black streaked dwarf virus (RBSDV), Fiji disease virus (FDV) and Nilaparvata lugens reovirus (NLRV) polymerases as well as to corresponding proteins of members of other genera of the Reoviridae. MRCV S2 codes for a protein with intermediate homology to the ones coded by RBSDV S4 and FDV S3 'B' spike, which is presumably the B-spike protein. MRCV S3 most probably encodes the major core protein and is highly homologous to corresponding proteins of RBSDV S2 and FDV S3. MRCV S6-encoded protein has low homology to the proteins of unknown function coded by RBSDV S6 and FDV S6. The identity levels between all analyzed MRCV coded proteins and their RBSDV counterparts varied between 84.5 and 44.8%. The analysis of the reported sequences allowed a phylogenetic comparison of MRCV with other reovirus and supported its taxonomic status within the genus. © 2002 Elsevier Science B.V. All rights reserved. Fil:Distéfano, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del Vas, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681702_v92_n1_p113_Distefano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
English |
| orig_language_str_mv |
English |
| topic |
Core protein Fijivirus MRCV Reoviridae RNA-dependent RNA polymerase core protein RNA directed RNA polymerase virus protein virus RNA amino acid sequence article controlled study figi disease virus gene sequence genetic conservation genomics mal de rio cuarto virus nilaparvata lugens reovirus nonhuman nucleotide sequence phylogeny priority journal protein function protein motif Reovirus rice black streaked dwarf virus RNA virus sequence analysis sequence homology virus genome virus identification Amino Acid Sequence Base Sequence Cloning, Molecular Conserved Sequence DNA, Viral Genome, Viral Molecular Sequence Data Phylogeny Reoviridae Sequence Homology, Amino Acid Species Specificity Viral Proteins Fiji disease virus Fijivirus Mal de Rio Cuarto virus Nilaparvata lugens Nilaparvata lugens reovirus Reoviridae Reovirus sp. Rice black streaked dwarf virus |
| spellingShingle |
Core protein Fijivirus MRCV Reoviridae RNA-dependent RNA polymerase core protein RNA directed RNA polymerase virus protein virus RNA amino acid sequence article controlled study figi disease virus gene sequence genetic conservation genomics mal de rio cuarto virus nilaparvata lugens reovirus nonhuman nucleotide sequence phylogeny priority journal protein function protein motif Reovirus rice black streaked dwarf virus RNA virus sequence analysis sequence homology virus genome virus identification Amino Acid Sequence Base Sequence Cloning, Molecular Conserved Sequence DNA, Viral Genome, Viral Molecular Sequence Data Phylogeny Reoviridae Sequence Homology, Amino Acid Species Specificity Viral Proteins Fiji disease virus Fijivirus Mal de Rio Cuarto virus Nilaparvata lugens Nilaparvata lugens reovirus Reoviridae Reovirus sp. Rice black streaked dwarf virus Distéfano, A.J. Conci, L.R. Muñoz Hidalgo, M. Guzmán, F.A. Hopp, H.E. Del Vas, M. Sequence and phylogenetic analysis of genome segments S1, S2, S3 and S6 of Mal de Río Cuarto virus, a newly accepted Fijivirus species |
| topic_facet |
Core protein Fijivirus MRCV Reoviridae RNA-dependent RNA polymerase core protein RNA directed RNA polymerase virus protein virus RNA amino acid sequence article controlled study figi disease virus gene sequence genetic conservation genomics mal de rio cuarto virus nilaparvata lugens reovirus nonhuman nucleotide sequence phylogeny priority journal protein function protein motif Reovirus rice black streaked dwarf virus RNA virus sequence analysis sequence homology virus genome virus identification Amino Acid Sequence Base Sequence Cloning, Molecular Conserved Sequence DNA, Viral Genome, Viral Molecular Sequence Data Phylogeny Reoviridae Sequence Homology, Amino Acid Species Specificity Viral Proteins Fiji disease virus Fijivirus Mal de Rio Cuarto virus Nilaparvata lugens Nilaparvata lugens reovirus Reoviridae Reovirus sp. Rice black streaked dwarf virus |
| description |
Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. The nucleotide sequence of four MRCV genome segments was determined. MRCV S1, S2, S3 and S6 were predicted to encode proteins of 168.4, 134.4, 141.7 and 90 kDa, respectively. MRCV S1 encodes a basic protein that contains conserved RNA-dependent RNA polymerase motifs, and is homologous to Rice black streaked dwarf virus (RBSDV), Fiji disease virus (FDV) and Nilaparvata lugens reovirus (NLRV) polymerases as well as to corresponding proteins of members of other genera of the Reoviridae. MRCV S2 codes for a protein with intermediate homology to the ones coded by RBSDV S4 and FDV S3 'B' spike, which is presumably the B-spike protein. MRCV S3 most probably encodes the major core protein and is highly homologous to corresponding proteins of RBSDV S2 and FDV S3. MRCV S6-encoded protein has low homology to the proteins of unknown function coded by RBSDV S6 and FDV S6. The identity levels between all analyzed MRCV coded proteins and their RBSDV counterparts varied between 84.5 and 44.8%. The analysis of the reported sequences allowed a phylogenetic comparison of MRCV with other reovirus and supported its taxonomic status within the genus. © 2002 Elsevier Science B.V. All rights reserved. |
| format |
JOUR |
| author |
Distéfano, A.J. Conci, L.R. Muñoz Hidalgo, M. Guzmán, F.A. Hopp, H.E. Del Vas, M. |
| author_facet |
Distéfano, A.J. Conci, L.R. Muñoz Hidalgo, M. Guzmán, F.A. Hopp, H.E. Del Vas, M. |
| author_sort |
Distéfano, A.J. |
| title |
Sequence and phylogenetic analysis of genome segments S1, S2, S3 and S6 of Mal de Río Cuarto virus, a newly accepted Fijivirus species |
| title_short |
Sequence and phylogenetic analysis of genome segments S1, S2, S3 and S6 of Mal de Río Cuarto virus, a newly accepted Fijivirus species |
| title_full |
Sequence and phylogenetic analysis of genome segments S1, S2, S3 and S6 of Mal de Río Cuarto virus, a newly accepted Fijivirus species |
| title_fullStr |
Sequence and phylogenetic analysis of genome segments S1, S2, S3 and S6 of Mal de Río Cuarto virus, a newly accepted Fijivirus species |
| title_full_unstemmed |
Sequence and phylogenetic analysis of genome segments S1, S2, S3 and S6 of Mal de Río Cuarto virus, a newly accepted Fijivirus species |
| title_sort |
sequence and phylogenetic analysis of genome segments s1, s2, s3 and s6 of mal de río cuarto virus, a newly accepted fijivirus species |
| url |
http://hdl.handle.net/20.500.12110/paper_01681702_v92_n1_p113_Distefano |
| work_keys_str_mv |
AT distefanoaj sequenceandphylogeneticanalysisofgenomesegmentss1s2s3ands6ofmalderiocuartovirusanewlyacceptedfijivirusspecies AT concilr sequenceandphylogeneticanalysisofgenomesegmentss1s2s3ands6ofmalderiocuartovirusanewlyacceptedfijivirusspecies AT munozhidalgom sequenceandphylogeneticanalysisofgenomesegmentss1s2s3ands6ofmalderiocuartovirusanewlyacceptedfijivirusspecies AT guzmanfa sequenceandphylogeneticanalysisofgenomesegmentss1s2s3ands6ofmalderiocuartovirusanewlyacceptedfijivirusspecies AT hopphe sequenceandphylogeneticanalysisofgenomesegmentss1s2s3ands6ofmalderiocuartovirusanewlyacceptedfijivirusspecies AT delvasm sequenceandphylogeneticanalysisofgenomesegmentss1s2s3ands6ofmalderiocuartovirusanewlyacceptedfijivirusspecies |
| _version_ |
1807320845557694464 |