Vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication
We detected apoptosis induction in the vesicular stomatitis virus (VSV) infected mammalian cell lines Vero-76, Cos-7, and BHK-21. Cell lines were analyzed by chromosomal DNA fragmentation and nuclear morphology. In order to determine the step in the viral cycle at which apoptosis of infected cells i...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01681702_v86_n1-2_p87_Gadaleta |
| Aporte de: |
| id |
todo:paper_01681702_v86_n1-2_p87_Gadaleta |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_01681702_v86_n1-2_p87_Gadaleta2023-10-03T15:06:17Z Vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication Gadaleta, P. Vacotto, M. Coulombié, F. Apoptosis Vesicular stomatitis virus Viral cycle ammonium chloride chemical agent cycloheximide DNA fragment virus protein animal cell apoptosis article cell assay cell death cell strain BHK cell strain COS7 chromosome analysis controlled study DNA determination gene structure mammal cell nonhuman priority journal protein synthesis structure analysis ultraviolet radiation Vero cell Vesicular stomatitis virus virus induction virus infection virus replication animal biosynthesis cell culture Cercopithecus physiology radiation exposure Mammalia Vesicular stomatitis virus Animals Apoptosis Cercopithecus aethiops Tumor Cells, Cultured Ultraviolet Rays Vero Cells Vesicular stomatitis-Indiana virus Viral Proteins Virus Replication We detected apoptosis induction in the vesicular stomatitis virus (VSV) infected mammalian cell lines Vero-76, Cos-7, and BHK-21. Cell lines were analyzed by chromosomal DNA fragmentation and nuclear morphology. In order to determine the step in the viral cycle at which apoptosis of infected cells is triggered, chemical and physical agents were used to block viral infection at different times and then the apoptotic response of infected cells was examined. The treatment of Vero-76 infected cells with a lysosomotrophic agent, such as NH4Cl, was shown to abrogate virus apoptosis induction. On the other hand, VSV-induced apoptosis was not blocked by the presence of cycloheximide, suggesting that the de novo viral protein synthesis is not required for this process. UV-inactivated viruses were also capable of inducing apoptosis in Vero-76 cells, indicating that the activation of a programmed cell death process by VSV does not require viral replication. We conclude from these findings that VSV induces apoptosis at early stages of infection. © 2002 Elsevier Science B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681702_v86_n1-2_p87_Gadaleta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptosis Vesicular stomatitis virus Viral cycle ammonium chloride chemical agent cycloheximide DNA fragment virus protein animal cell apoptosis article cell assay cell death cell strain BHK cell strain COS7 chromosome analysis controlled study DNA determination gene structure mammal cell nonhuman priority journal protein synthesis structure analysis ultraviolet radiation Vero cell Vesicular stomatitis virus virus induction virus infection virus replication animal biosynthesis cell culture Cercopithecus physiology radiation exposure Mammalia Vesicular stomatitis virus Animals Apoptosis Cercopithecus aethiops Tumor Cells, Cultured Ultraviolet Rays Vero Cells Vesicular stomatitis-Indiana virus Viral Proteins Virus Replication |
| spellingShingle |
Apoptosis Vesicular stomatitis virus Viral cycle ammonium chloride chemical agent cycloheximide DNA fragment virus protein animal cell apoptosis article cell assay cell death cell strain BHK cell strain COS7 chromosome analysis controlled study DNA determination gene structure mammal cell nonhuman priority journal protein synthesis structure analysis ultraviolet radiation Vero cell Vesicular stomatitis virus virus induction virus infection virus replication animal biosynthesis cell culture Cercopithecus physiology radiation exposure Mammalia Vesicular stomatitis virus Animals Apoptosis Cercopithecus aethiops Tumor Cells, Cultured Ultraviolet Rays Vero Cells Vesicular stomatitis-Indiana virus Viral Proteins Virus Replication Gadaleta, P. Vacotto, M. Coulombié, F. Vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication |
| topic_facet |
Apoptosis Vesicular stomatitis virus Viral cycle ammonium chloride chemical agent cycloheximide DNA fragment virus protein animal cell apoptosis article cell assay cell death cell strain BHK cell strain COS7 chromosome analysis controlled study DNA determination gene structure mammal cell nonhuman priority journal protein synthesis structure analysis ultraviolet radiation Vero cell Vesicular stomatitis virus virus induction virus infection virus replication animal biosynthesis cell culture Cercopithecus physiology radiation exposure Mammalia Vesicular stomatitis virus Animals Apoptosis Cercopithecus aethiops Tumor Cells, Cultured Ultraviolet Rays Vero Cells Vesicular stomatitis-Indiana virus Viral Proteins Virus Replication |
| description |
We detected apoptosis induction in the vesicular stomatitis virus (VSV) infected mammalian cell lines Vero-76, Cos-7, and BHK-21. Cell lines were analyzed by chromosomal DNA fragmentation and nuclear morphology. In order to determine the step in the viral cycle at which apoptosis of infected cells is triggered, chemical and physical agents were used to block viral infection at different times and then the apoptotic response of infected cells was examined. The treatment of Vero-76 infected cells with a lysosomotrophic agent, such as NH4Cl, was shown to abrogate virus apoptosis induction. On the other hand, VSV-induced apoptosis was not blocked by the presence of cycloheximide, suggesting that the de novo viral protein synthesis is not required for this process. UV-inactivated viruses were also capable of inducing apoptosis in Vero-76 cells, indicating that the activation of a programmed cell death process by VSV does not require viral replication. We conclude from these findings that VSV induces apoptosis at early stages of infection. © 2002 Elsevier Science B.V. All rights reserved. |
| format |
JOUR |
| author |
Gadaleta, P. Vacotto, M. Coulombié, F. |
| author_facet |
Gadaleta, P. Vacotto, M. Coulombié, F. |
| author_sort |
Gadaleta, P. |
| title |
Vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication |
| title_short |
Vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication |
| title_full |
Vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication |
| title_fullStr |
Vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication |
| title_full_unstemmed |
Vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication |
| title_sort |
vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication |
| url |
http://hdl.handle.net/20.500.12110/paper_01681702_v86_n1-2_p87_Gadaleta |
| work_keys_str_mv |
AT gadaletap vesicularstomatitisvirusinducesapoptosisatearlystagesintheviralcycleanddoesnotdependonvirusreplication AT vacottom vesicularstomatitisvirusinducesapoptosisatearlystagesintheviralcycleanddoesnotdependonvirusreplication AT coulombief vesicularstomatitisvirusinducesapoptosisatearlystagesintheviralcycleanddoesnotdependonvirusreplication |
| _version_ |
1807322161312956416 |