Kluyvera bacteriophage Kvp1: A new member of the Podoviridae family phylogenetically related to the coliphage T7

A DNA containing bacteriophage, Kvp1, was isolated from the water of a very polluted river, the Matanza river, near the central district of Buenos Aires City. This bacteriophage infects bacteria belonging to the Kluyvera cryocrescens species (strain 21g) isolated from the same river. Kvp1 is a lytic...

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Detalles Bibliográficos
Autores principales:	Gadaleta, P., Zorzópulos, J.
Formato:	JOUR
Materias:	Kluyvera Phylogenetic relationships Podoviridae T7 bacteriophage article bacteriophage bacteriophage t7 coliphage controlled study nonhuman phylogeny polyacrylamide gel electrophoresis priority journal Bacteriophage T7 Bacteriophages DNA DNA, Viral Enterobacteriaceae Genome, Viral Microscopy, Electron Molecular Sequence Data Phylogeny Viral Proteins Bacteria (microorganisms) Bacteriophage Kvp1 Kluyvera cryocrescens
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01681702_v51_n1_p43_Gadaleta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01681702_v51_n1_p43_Gadaleta
record_format	dspace
spelling	todo:paper_01681702_v51_n1_p43_Gadaleta2023-10-03T15:06:17Z Kluyvera bacteriophage Kvp1: A new member of the Podoviridae family phylogenetically related to the coliphage T7 Gadaleta, P. Zorzópulos, J. Kluyvera Phylogenetic relationships Podoviridae T7 bacteriophage article bacteriophage bacteriophage t7 coliphage controlled study nonhuman phylogeny polyacrylamide gel electrophoresis priority journal Bacteriophage T7 Bacteriophages DNA DNA, Viral Enterobacteriaceae Genome, Viral Microscopy, Electron Molecular Sequence Data Phylogeny Podoviridae Viral Proteins Bacteria (microorganisms) Bacteriophage Kvp1 Kluyvera Kluyvera cryocrescens Podoviridae A DNA containing bacteriophage, Kvp1, was isolated from the water of a very polluted river, the Matanza river, near the central district of Buenos Aires City. This bacteriophage infects bacteria belonging to the Kluyvera cryocrescens species (strain 21g) isolated from the same river. Kvp1 is a lytic bacteriophage and its propagation characteristics are: burst size 30, latent period 13 min and rise period 10 min. Morphologically, Kvp1 is a small icosahedral bacteriophage, 59.1 nm in diameter, which possesses a short wedge-shaped tail. Its buoyant density in CICs is 1.517 g/cm3. Kvp1 DNA is linear, double stranded and approximately 40,000 bp in size. The viral particle is composed of at least nine proteins. SDS-PAGE patterns of these proteins and of those produced during the host infection, in addition to its morphological and genomic characteristics, suggested that Kvp1 is similar to the coliphage T7. Molecular cloning, sequencing and computer-assisted analysis of Kvp1 DNA fragments confirmed the relationship to the coliphage. Taking this into account, the partial sequence of the phage RNA polymerase was used to construct phylogenetic relationships between Kvp1 and other related phages. To our knowledge, Kvp1 is the first bacteriophage described which uses as host a member of the Kluyvera bacterial genus. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681702_v51_n1_p43_Gadaleta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Kluyvera Phylogenetic relationships Podoviridae T7 bacteriophage article bacteriophage bacteriophage t7 coliphage controlled study nonhuman phylogeny polyacrylamide gel electrophoresis priority journal Bacteriophage T7 Bacteriophages DNA DNA, Viral Enterobacteriaceae Genome, Viral Microscopy, Electron Molecular Sequence Data Phylogeny Podoviridae Viral Proteins Bacteria (microorganisms) Bacteriophage Kvp1 Kluyvera Kluyvera cryocrescens Podoviridae
spellingShingle	Kluyvera Phylogenetic relationships Podoviridae T7 bacteriophage article bacteriophage bacteriophage t7 coliphage controlled study nonhuman phylogeny polyacrylamide gel electrophoresis priority journal Bacteriophage T7 Bacteriophages DNA DNA, Viral Enterobacteriaceae Genome, Viral Microscopy, Electron Molecular Sequence Data Phylogeny Podoviridae Viral Proteins Bacteria (microorganisms) Bacteriophage Kvp1 Kluyvera Kluyvera cryocrescens Podoviridae Gadaleta, P. Zorzópulos, J. Kluyvera bacteriophage Kvp1: A new member of the Podoviridae family phylogenetically related to the coliphage T7
topic_facet	Kluyvera Phylogenetic relationships Podoviridae T7 bacteriophage article bacteriophage bacteriophage t7 coliphage controlled study nonhuman phylogeny polyacrylamide gel electrophoresis priority journal Bacteriophage T7 Bacteriophages DNA DNA, Viral Enterobacteriaceae Genome, Viral Microscopy, Electron Molecular Sequence Data Phylogeny Podoviridae Viral Proteins Bacteria (microorganisms) Bacteriophage Kvp1 Kluyvera Kluyvera cryocrescens Podoviridae
description	A DNA containing bacteriophage, Kvp1, was isolated from the water of a very polluted river, the Matanza river, near the central district of Buenos Aires City. This bacteriophage infects bacteria belonging to the Kluyvera cryocrescens species (strain 21g) isolated from the same river. Kvp1 is a lytic bacteriophage and its propagation characteristics are: burst size 30, latent period 13 min and rise period 10 min. Morphologically, Kvp1 is a small icosahedral bacteriophage, 59.1 nm in diameter, which possesses a short wedge-shaped tail. Its buoyant density in CICs is 1.517 g/cm3. Kvp1 DNA is linear, double stranded and approximately 40,000 bp in size. The viral particle is composed of at least nine proteins. SDS-PAGE patterns of these proteins and of those produced during the host infection, in addition to its morphological and genomic characteristics, suggested that Kvp1 is similar to the coliphage T7. Molecular cloning, sequencing and computer-assisted analysis of Kvp1 DNA fragments confirmed the relationship to the coliphage. Taking this into account, the partial sequence of the phage RNA polymerase was used to construct phylogenetic relationships between Kvp1 and other related phages. To our knowledge, Kvp1 is the first bacteriophage described which uses as host a member of the Kluyvera bacterial genus.
format	JOUR
author	Gadaleta, P. Zorzópulos, J.
author_facet	Gadaleta, P. Zorzópulos, J.
author_sort	Gadaleta, P.
title	Kluyvera bacteriophage Kvp1: A new member of the Podoviridae family phylogenetically related to the coliphage T7
title_short	Kluyvera bacteriophage Kvp1: A new member of the Podoviridae family phylogenetically related to the coliphage T7
title_full	Kluyvera bacteriophage Kvp1: A new member of the Podoviridae family phylogenetically related to the coliphage T7
title_fullStr	Kluyvera bacteriophage Kvp1: A new member of the Podoviridae family phylogenetically related to the coliphage T7
title_full_unstemmed	Kluyvera bacteriophage Kvp1: A new member of the Podoviridae family phylogenetically related to the coliphage T7
title_sort	kluyvera bacteriophage kvp1: a new member of the podoviridae family phylogenetically related to the coliphage t7
url	http://hdl.handle.net/20.500.12110/paper_01681702_v51_n1_p43_Gadaleta
work_keys_str_mv	AT gadaletap kluyverabacteriophagekvp1anewmemberofthepodoviridaefamilyphylogeneticallyrelatedtothecoliphaget7 AT zorzopulosj kluyverabacteriophagekvp1anewmemberofthepodoviridaefamilyphylogeneticallyrelatedtothecoliphaget7
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Kluyvera bacteriophage Kvp1: A new member of the Podoviridae family phylogenetically related to the coliphage T7

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