Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV...
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todo:paper_01681702_v242_n_p122_Maidana2023-10-03T15:06:16Z Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 Maidana, S.S. Craig, P.O. Craig, M.I. Ludwig, L. Mauroy, A. Thiry, E. Romera, S.A. Alphaherpesviruses Bovine herpesvirus 1 (BoHV-1) Bovine herpesvirus 5 (BoHV-5) Glycoprotein B Natural recombination neutralizing antibody virus DNA Alphaherpesvirinae amino acid sequence animal cell Article bovine alphaherpesvirus 1 bovine alphaherpesvirus 5 comparative study DNA sequence genetic code genetic recombination homologous recombination MDBK cell line molecular model nonhuman open reading frame phylogenetic tree phylogeny priority journal virus isolation virus neutralization virus recombinant animal bovine Bovine herpesvirus 1 Bovine herpesvirus 5 chemistry classification cluster analysis genetic variation genetics genotype homologous recombination isolation and purification polymerase chain reaction Animals Cattle Cluster Analysis DNA, Viral Genetic Variation Genotype Herpesvirus 1, Bovine Herpesvirus 5, Bovine Homologous Recombination Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 and BoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene. We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays. Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5. © 2017 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681702_v242_n_p122_Maidana |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alphaherpesviruses Bovine herpesvirus 1 (BoHV-1) Bovine herpesvirus 5 (BoHV-5) Glycoprotein B Natural recombination neutralizing antibody virus DNA Alphaherpesvirinae amino acid sequence animal cell Article bovine alphaherpesvirus 1 bovine alphaherpesvirus 5 comparative study DNA sequence genetic code genetic recombination homologous recombination MDBK cell line molecular model nonhuman open reading frame phylogenetic tree phylogeny priority journal virus isolation virus neutralization virus recombinant animal bovine Bovine herpesvirus 1 Bovine herpesvirus 5 chemistry classification cluster analysis genetic variation genetics genotype homologous recombination isolation and purification polymerase chain reaction Animals Cattle Cluster Analysis DNA, Viral Genetic Variation Genotype Herpesvirus 1, Bovine Herpesvirus 5, Bovine Homologous Recombination Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA |
| spellingShingle |
Alphaherpesviruses Bovine herpesvirus 1 (BoHV-1) Bovine herpesvirus 5 (BoHV-5) Glycoprotein B Natural recombination neutralizing antibody virus DNA Alphaherpesvirinae amino acid sequence animal cell Article bovine alphaherpesvirus 1 bovine alphaherpesvirus 5 comparative study DNA sequence genetic code genetic recombination homologous recombination MDBK cell line molecular model nonhuman open reading frame phylogenetic tree phylogeny priority journal virus isolation virus neutralization virus recombinant animal bovine Bovine herpesvirus 1 Bovine herpesvirus 5 chemistry classification cluster analysis genetic variation genetics genotype homologous recombination isolation and purification polymerase chain reaction Animals Cattle Cluster Analysis DNA, Viral Genetic Variation Genotype Herpesvirus 1, Bovine Herpesvirus 5, Bovine Homologous Recombination Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Maidana, S.S. Craig, P.O. Craig, M.I. Ludwig, L. Mauroy, A. Thiry, E. Romera, S.A. Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| topic_facet |
Alphaherpesviruses Bovine herpesvirus 1 (BoHV-1) Bovine herpesvirus 5 (BoHV-5) Glycoprotein B Natural recombination neutralizing antibody virus DNA Alphaherpesvirinae amino acid sequence animal cell Article bovine alphaherpesvirus 1 bovine alphaherpesvirus 5 comparative study DNA sequence genetic code genetic recombination homologous recombination MDBK cell line molecular model nonhuman open reading frame phylogenetic tree phylogeny priority journal virus isolation virus neutralization virus recombinant animal bovine Bovine herpesvirus 1 Bovine herpesvirus 5 chemistry classification cluster analysis genetic variation genetics genotype homologous recombination isolation and purification polymerase chain reaction Animals Cattle Cluster Analysis DNA, Viral Genetic Variation Genotype Herpesvirus 1, Bovine Herpesvirus 5, Bovine Homologous Recombination Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA |
| description |
Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 and BoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene. We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays. Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5. © 2017 Elsevier B.V. |
| format |
JOUR |
| author |
Maidana, S.S. Craig, P.O. Craig, M.I. Ludwig, L. Mauroy, A. Thiry, E. Romera, S.A. |
| author_facet |
Maidana, S.S. Craig, P.O. Craig, M.I. Ludwig, L. Mauroy, A. Thiry, E. Romera, S.A. |
| author_sort |
Maidana, S.S. |
| title |
Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| title_short |
Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| title_full |
Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| title_fullStr |
Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| title_full_unstemmed |
Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| title_sort |
evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| url |
http://hdl.handle.net/20.500.12110/paper_01681702_v242_n_p122_Maidana |
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