The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing
Plants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense, viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, the P0 proteins encoded by poleroviruses have been identified as SSP. In this study, we demonstrated t...
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todo:paper_01681702_v180_n_p70_Delfosse2023-10-03T15:06:14Z The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing Delfosse, V.C. Agrofoglio, Y.C. Casse, M.F. Kresic, I.B. Hopp, H.E. Ziegler-Graff, V. Distéfano, A.J. CLRDV P0 protein Polerovirus Silencing suppressor P0 protein RNA small interfering RNA unclassified drug virus protein Agrobacterium article cotton leafroll dwarf virus enzyme inhibition inoculation inverted repeat molecular cloning Nicotiana benthamiana nonhuman Northern blotting Polerovirus priority journal transgene virus genome CLRDV P0 protein Polerovirus Silencing suppressor Agrobacterium Host-Pathogen Interactions Luteoviridae RNA Interference Tobacco Transgenes Viral Proteins Plants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense, viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, the P0 proteins encoded by poleroviruses have been identified as SSP. In this study, we demonstrated that cotton leafroll dwarf virus (CLRDV, genus Polerovirus) P0 protein suppressed local silencing that was induced by sense or inverted repeat transgenes in Agrobacterium co-infiltration assay in Nicotiana benthamiana plants. A CLRDV full-length infectious cDNA clone that is able to infect N. benthamiana through Agrobacterium-mediated inoculation also inhibited local silencing in co-infiltration assays, suggesting that the P0 protein exhibits similar RNA silencing suppression activity when expressed from the full-length viral genome. On the other hand, the P0 protein did not efficiently inhibit the spread of systemic silencing signals. Moreover, Northern blotting indicated that the P0 protein inhibits the generation of secondary but not primary small interfering RNAs. The study of CLRDV P0 suppression activity may contribute to understanding the molecular mechanisms involved in the induction of cotton blue disease by CLRDV infection. © 2013 Elsevier B.V. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Distéfano, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681702_v180_n_p70_Delfosse |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CLRDV P0 protein Polerovirus Silencing suppressor P0 protein RNA small interfering RNA unclassified drug virus protein Agrobacterium article cotton leafroll dwarf virus enzyme inhibition inoculation inverted repeat molecular cloning Nicotiana benthamiana nonhuman Northern blotting Polerovirus priority journal transgene virus genome CLRDV P0 protein Polerovirus Silencing suppressor Agrobacterium Host-Pathogen Interactions Luteoviridae RNA Interference Tobacco Transgenes Viral Proteins |
| spellingShingle |
CLRDV P0 protein Polerovirus Silencing suppressor P0 protein RNA small interfering RNA unclassified drug virus protein Agrobacterium article cotton leafroll dwarf virus enzyme inhibition inoculation inverted repeat molecular cloning Nicotiana benthamiana nonhuman Northern blotting Polerovirus priority journal transgene virus genome CLRDV P0 protein Polerovirus Silencing suppressor Agrobacterium Host-Pathogen Interactions Luteoviridae RNA Interference Tobacco Transgenes Viral Proteins Delfosse, V.C. Agrofoglio, Y.C. Casse, M.F. Kresic, I.B. Hopp, H.E. Ziegler-Graff, V. Distéfano, A.J. The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| topic_facet |
CLRDV P0 protein Polerovirus Silencing suppressor P0 protein RNA small interfering RNA unclassified drug virus protein Agrobacterium article cotton leafroll dwarf virus enzyme inhibition inoculation inverted repeat molecular cloning Nicotiana benthamiana nonhuman Northern blotting Polerovirus priority journal transgene virus genome CLRDV P0 protein Polerovirus Silencing suppressor Agrobacterium Host-Pathogen Interactions Luteoviridae RNA Interference Tobacco Transgenes Viral Proteins |
| description |
Plants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense, viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, the P0 proteins encoded by poleroviruses have been identified as SSP. In this study, we demonstrated that cotton leafroll dwarf virus (CLRDV, genus Polerovirus) P0 protein suppressed local silencing that was induced by sense or inverted repeat transgenes in Agrobacterium co-infiltration assay in Nicotiana benthamiana plants. A CLRDV full-length infectious cDNA clone that is able to infect N. benthamiana through Agrobacterium-mediated inoculation also inhibited local silencing in co-infiltration assays, suggesting that the P0 protein exhibits similar RNA silencing suppression activity when expressed from the full-length viral genome. On the other hand, the P0 protein did not efficiently inhibit the spread of systemic silencing signals. Moreover, Northern blotting indicated that the P0 protein inhibits the generation of secondary but not primary small interfering RNAs. The study of CLRDV P0 suppression activity may contribute to understanding the molecular mechanisms involved in the induction of cotton blue disease by CLRDV infection. © 2013 Elsevier B.V. |
| format |
JOUR |
| author |
Delfosse, V.C. Agrofoglio, Y.C. Casse, M.F. Kresic, I.B. Hopp, H.E. Ziegler-Graff, V. Distéfano, A.J. |
| author_facet |
Delfosse, V.C. Agrofoglio, Y.C. Casse, M.F. Kresic, I.B. Hopp, H.E. Ziegler-Graff, V. Distéfano, A.J. |
| author_sort |
Delfosse, V.C. |
| title |
The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_short |
The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_full |
The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_fullStr |
The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_full_unstemmed |
The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_sort |
p0 protein encoded by cotton leafroll dwarf virus (clrdv) inhibits local but not systemic rna silencing |
| url |
http://hdl.handle.net/20.500.12110/paper_01681702_v180_n_p70_Delfosse |
| work_keys_str_mv |
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