Infectious dengue-1 virus entry into mosquito C6/36 cells
The entry of dengue virus-1 (DENV-1) strain Hawaii into mosquito C6/36 cells was analyzed using a variety of biochemical inhibitors together with electron microscopy. The treatment with ammonium chloride, chlorpromazine, dansylcadaverine and dynasore inhibited virus yields, determined by infectivity...
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todo:paper_01681702_v160_n1-2_p173_Acosta2023-10-03T15:06:13Z Infectious dengue-1 virus entry into mosquito C6/36 cells Acosta, E.G. Castilla, V. Damonte, E.B. Clathrin-mediated endocytosis Dengue virus-1 Mosquito cells Virus entry ammonium chloride chlorpromazine clathrin dansylcadaverine dynamin enzyme inhibitor methyl beta cyclodextrin reagent animal cell article cell membrane coated vesicle controlled study dengue Dengue virus 1 Dengue virus 3 Dengue virus 4 electron microscopy endocytosis immunofluorescence insect cell insect cell culture mosquito nonhuman pH priority journal virion virus entry virus isolation virus strain virus titration Animals Cell Line Clathrin-Coated Vesicles Culicidae Dengue Virus Endocytosis Microscopy, Electron Virus Internalization Dengue virus The entry of dengue virus-1 (DENV-1) strain Hawaii into mosquito C6/36 cells was analyzed using a variety of biochemical inhibitors together with electron microscopy. The treatment with ammonium chloride, chlorpromazine, dansylcadaverine and dynasore inhibited virus yields, determined by infectivity titrations, whereas nystatin and methyl-β-cyclodextrin did not have any effect. The effect of the clathrin and dynamin inhibitors on DENV-1 entry was corroborated by detection of internalized virions using immunofluorescence staining. Furthermore, electron micrographs showed the incoming virions attached to electron-dense invaginations of the plasma membrane and within coated vesicles that resembled clathrin-coated pits and vesicles, respectively. The susceptibility to clathrin and dynamin inhibitors of clinical isolates from recent outbreaks was comparable to that shown by the cell culture-adapted reference strain. Similarly, DENV-3 strain H87 and DENV-4 strain 8124 were also inhibited in the presence of ammonium chloride, chlorpromazine and dynasore, allowing conclude that the infectious entry of DENV serotypes to mosquito cells occurs by low pH-dependent clathrin-mediated endocytosis. © 2011 Elsevier B.V. Fil:Acosta, E.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681702_v160_n1-2_p173_Acosta |
| institution |
Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Clathrin-mediated endocytosis Dengue virus-1 Mosquito cells Virus entry ammonium chloride chlorpromazine clathrin dansylcadaverine dynamin enzyme inhibitor methyl beta cyclodextrin reagent animal cell article cell membrane coated vesicle controlled study dengue Dengue virus 1 Dengue virus 3 Dengue virus 4 electron microscopy endocytosis immunofluorescence insect cell insect cell culture mosquito nonhuman pH priority journal virion virus entry virus isolation virus strain virus titration Animals Cell Line Clathrin-Coated Vesicles Culicidae Dengue Virus Endocytosis Microscopy, Electron Virus Internalization Dengue virus |
| spellingShingle |
Clathrin-mediated endocytosis Dengue virus-1 Mosquito cells Virus entry ammonium chloride chlorpromazine clathrin dansylcadaverine dynamin enzyme inhibitor methyl beta cyclodextrin reagent animal cell article cell membrane coated vesicle controlled study dengue Dengue virus 1 Dengue virus 3 Dengue virus 4 electron microscopy endocytosis immunofluorescence insect cell insect cell culture mosquito nonhuman pH priority journal virion virus entry virus isolation virus strain virus titration Animals Cell Line Clathrin-Coated Vesicles Culicidae Dengue Virus Endocytosis Microscopy, Electron Virus Internalization Dengue virus Acosta, E.G. Castilla, V. Damonte, E.B. Infectious dengue-1 virus entry into mosquito C6/36 cells |
| topic_facet |
Clathrin-mediated endocytosis Dengue virus-1 Mosquito cells Virus entry ammonium chloride chlorpromazine clathrin dansylcadaverine dynamin enzyme inhibitor methyl beta cyclodextrin reagent animal cell article cell membrane coated vesicle controlled study dengue Dengue virus 1 Dengue virus 3 Dengue virus 4 electron microscopy endocytosis immunofluorescence insect cell insect cell culture mosquito nonhuman pH priority journal virion virus entry virus isolation virus strain virus titration Animals Cell Line Clathrin-Coated Vesicles Culicidae Dengue Virus Endocytosis Microscopy, Electron Virus Internalization Dengue virus |
| description |
The entry of dengue virus-1 (DENV-1) strain Hawaii into mosquito C6/36 cells was analyzed using a variety of biochemical inhibitors together with electron microscopy. The treatment with ammonium chloride, chlorpromazine, dansylcadaverine and dynasore inhibited virus yields, determined by infectivity titrations, whereas nystatin and methyl-β-cyclodextrin did not have any effect. The effect of the clathrin and dynamin inhibitors on DENV-1 entry was corroborated by detection of internalized virions using immunofluorescence staining. Furthermore, electron micrographs showed the incoming virions attached to electron-dense invaginations of the plasma membrane and within coated vesicles that resembled clathrin-coated pits and vesicles, respectively. The susceptibility to clathrin and dynamin inhibitors of clinical isolates from recent outbreaks was comparable to that shown by the cell culture-adapted reference strain. Similarly, DENV-3 strain H87 and DENV-4 strain 8124 were also inhibited in the presence of ammonium chloride, chlorpromazine and dynasore, allowing conclude that the infectious entry of DENV serotypes to mosquito cells occurs by low pH-dependent clathrin-mediated endocytosis. © 2011 Elsevier B.V. |
| format |
JOUR |
| author |
Acosta, E.G. Castilla, V. Damonte, E.B. |
| author_facet |
Acosta, E.G. Castilla, V. Damonte, E.B. |
| author_sort |
Acosta, E.G. |
| title |
Infectious dengue-1 virus entry into mosquito C6/36 cells |
| title_short |
Infectious dengue-1 virus entry into mosquito C6/36 cells |
| title_full |
Infectious dengue-1 virus entry into mosquito C6/36 cells |
| title_fullStr |
Infectious dengue-1 virus entry into mosquito C6/36 cells |
| title_full_unstemmed |
Infectious dengue-1 virus entry into mosquito C6/36 cells |
| title_sort |
infectious dengue-1 virus entry into mosquito c6/36 cells |
| url |
http://hdl.handle.net/20.500.12110/paper_01681702_v160_n1-2_p173_Acosta |
| work_keys_str_mv |
AT acostaeg infectiousdengue1virusentryintomosquitoc636cells AT castillav infectiousdengue1virusentryintomosquitoc636cells AT damonteeb infectiousdengue1virusentryintomosquitoc636cells |
| _version_ |
1807317700964253696 |