Sol-gel silica platforms for microalgae-based optical biosensors

An advanced hybrid biosensing platform with improved optical quality is developed based on the acidic encapsulation of microalgi in silica matrices synthesized by TAFR (tetraethoxysilane derived alcohol free route). The three microalgi (Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamyd...

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Autores principales:	Perullini, M., Ferro, Y., Durrieu, C., Jobbágy, M., Bilmes, S.A.
Formato:	JOUR
Materias:	Biosensor Esterase activity Microalgae Optical quality Sol-gel Algae Microorganisms Silica Sol-gel process Sol-gels Biosensing platforms Chlamydomonas reinhardtii Esterase activities Micro-algae Optical bio-sensors Optical detection methods Optical qualities Pseudokirchneriella subcapitata Biosensors alginic acid calcium alginate silicate silicon dioxide tetraethoxysilane esterase glucuronic acid hexuronic acid silica gel water pollutant algal growth article biocompatibility Chlorella vulgaris elementary particle encapsulation fluorescence gelation growth inhibition hydrogel nonhuman optical biosensor pH polymerization priority journal Selenastrum capricornutum synthesis chemistry enzymology genetic procedures isolation and purification metabolism microalga procedures small angle scattering X ray diffraction Alginates Biosensing Techniques Esterases Glucuronic Acid Hexuronic Acids Scattering, Small Angle Silica Gel Water Pollutants, Chemical X-Ray Diffraction
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01681656_v179_n1_p65_Perullini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01681656_v179_n1_p65_Perullini
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spelling	todo:paper_01681656_v179_n1_p65_Perullini2023-10-03T15:06:07Z Sol-gel silica platforms for microalgae-based optical biosensors Perullini, M. Ferro, Y. Durrieu, C. Jobbágy, M. Bilmes, S.A. Biosensor Esterase activity Microalgae Optical quality Sol-gel Algae Microorganisms Silica Sol-gel process Sol-gels Biosensing platforms Chlamydomonas reinhardtii Esterase activities Micro-algae Optical bio-sensors Optical detection methods Optical qualities Pseudokirchneriella subcapitata Biosensors alginic acid calcium alginate silicate silicon dioxide tetraethoxysilane esterase glucuronic acid hexuronic acid silica gel water pollutant algal growth article biocompatibility Chlamydomonas reinhardtii Chlorella vulgaris elementary particle encapsulation fluorescence gelation growth inhibition hydrogel nonhuman optical biosensor pH polymerization priority journal Selenastrum capricornutum synthesis chemistry enzymology genetic procedures isolation and purification metabolism microalga procedures small angle scattering water pollutant X ray diffraction Alginates Biosensing Techniques Esterases Glucuronic Acid Hexuronic Acids Microalgae Scattering, Small Angle Silica Gel Water Pollutants, Chemical X-Ray Diffraction An advanced hybrid biosensing platform with improved optical quality is developed based on the acidic encapsulation of microalgi in silica matrices synthesized by TAFR (tetraethoxysilane derived alcohol free route). The three microalgi (Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii) were previously immobilized in alginate following the two-step procedure. Tuning the alginate protecting function with the aid of Tris-HCl buffer, the sol-gel synthesis was conducted at pH 4.0 well below the tolerance limit imposed by the encapsulated microalgae. The acidic condensation of Si(IV) generates silica matrices with outstanding optical properties that suit the requirements of biosensors based on optical detection methods. © 2014 Elsevier B.V. Fil:Perullini, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681656_v179_n1_p65_Perullini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Biosensor Esterase activity Microalgae Optical quality Sol-gel Algae Microorganisms Silica Sol-gel process Sol-gels Biosensing platforms Chlamydomonas reinhardtii Esterase activities Micro-algae Optical bio-sensors Optical detection methods Optical qualities Pseudokirchneriella subcapitata Biosensors alginic acid calcium alginate silicate silicon dioxide tetraethoxysilane esterase glucuronic acid hexuronic acid silica gel water pollutant algal growth article biocompatibility Chlamydomonas reinhardtii Chlorella vulgaris elementary particle encapsulation fluorescence gelation growth inhibition hydrogel nonhuman optical biosensor pH polymerization priority journal Selenastrum capricornutum synthesis chemistry enzymology genetic procedures isolation and purification metabolism microalga procedures small angle scattering water pollutant X ray diffraction Alginates Biosensing Techniques Esterases Glucuronic Acid Hexuronic Acids Microalgae Scattering, Small Angle Silica Gel Water Pollutants, Chemical X-Ray Diffraction
spellingShingle	Biosensor Esterase activity Microalgae Optical quality Sol-gel Algae Microorganisms Silica Sol-gel process Sol-gels Biosensing platforms Chlamydomonas reinhardtii Esterase activities Micro-algae Optical bio-sensors Optical detection methods Optical qualities Pseudokirchneriella subcapitata Biosensors alginic acid calcium alginate silicate silicon dioxide tetraethoxysilane esterase glucuronic acid hexuronic acid silica gel water pollutant algal growth article biocompatibility Chlamydomonas reinhardtii Chlorella vulgaris elementary particle encapsulation fluorescence gelation growth inhibition hydrogel nonhuman optical biosensor pH polymerization priority journal Selenastrum capricornutum synthesis chemistry enzymology genetic procedures isolation and purification metabolism microalga procedures small angle scattering water pollutant X ray diffraction Alginates Biosensing Techniques Esterases Glucuronic Acid Hexuronic Acids Microalgae Scattering, Small Angle Silica Gel Water Pollutants, Chemical X-Ray Diffraction Perullini, M. Ferro, Y. Durrieu, C. Jobbágy, M. Bilmes, S.A. Sol-gel silica platforms for microalgae-based optical biosensors
topic_facet	Biosensor Esterase activity Microalgae Optical quality Sol-gel Algae Microorganisms Silica Sol-gel process Sol-gels Biosensing platforms Chlamydomonas reinhardtii Esterase activities Micro-algae Optical bio-sensors Optical detection methods Optical qualities Pseudokirchneriella subcapitata Biosensors alginic acid calcium alginate silicate silicon dioxide tetraethoxysilane esterase glucuronic acid hexuronic acid silica gel water pollutant algal growth article biocompatibility Chlamydomonas reinhardtii Chlorella vulgaris elementary particle encapsulation fluorescence gelation growth inhibition hydrogel nonhuman optical biosensor pH polymerization priority journal Selenastrum capricornutum synthesis chemistry enzymology genetic procedures isolation and purification metabolism microalga procedures small angle scattering water pollutant X ray diffraction Alginates Biosensing Techniques Esterases Glucuronic Acid Hexuronic Acids Microalgae Scattering, Small Angle Silica Gel Water Pollutants, Chemical X-Ray Diffraction
description	An advanced hybrid biosensing platform with improved optical quality is developed based on the acidic encapsulation of microalgi in silica matrices synthesized by TAFR (tetraethoxysilane derived alcohol free route). The three microalgi (Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii) were previously immobilized in alginate following the two-step procedure. Tuning the alginate protecting function with the aid of Tris-HCl buffer, the sol-gel synthesis was conducted at pH 4.0 well below the tolerance limit imposed by the encapsulated microalgae. The acidic condensation of Si(IV) generates silica matrices with outstanding optical properties that suit the requirements of biosensors based on optical detection methods. © 2014 Elsevier B.V.
format	JOUR
author	Perullini, M. Ferro, Y. Durrieu, C. Jobbágy, M. Bilmes, S.A.
author_facet	Perullini, M. Ferro, Y. Durrieu, C. Jobbágy, M. Bilmes, S.A.
author_sort	Perullini, M.
title	Sol-gel silica platforms for microalgae-based optical biosensors
title_short	Sol-gel silica platforms for microalgae-based optical biosensors
title_full	Sol-gel silica platforms for microalgae-based optical biosensors
title_fullStr	Sol-gel silica platforms for microalgae-based optical biosensors
title_full_unstemmed	Sol-gel silica platforms for microalgae-based optical biosensors
title_sort	sol-gel silica platforms for microalgae-based optical biosensors
url	http://hdl.handle.net/20.500.12110/paper_01681656_v179_n1_p65_Perullini
work_keys_str_mv	AT perullinim solgelsilicaplatformsformicroalgaebasedopticalbiosensors AT ferroy solgelsilicaplatformsformicroalgaebasedopticalbiosensors AT durrieuc solgelsilicaplatformsformicroalgaebasedopticalbiosensors AT jobbagym solgelsilicaplatformsformicroalgaebasedopticalbiosensors AT bilmessa solgelsilicaplatformsformicroalgaebasedopticalbiosensors
_version_	1782029437807624192

Sol-gel silica platforms for microalgae-based optical biosensors

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