High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow
Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth...
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todo:paper_01681656_v124_n2_p469_Salamone2023-10-03T15:06:05Z High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow Salamone, D. Barañao, L. Santos, C. Bussmann, L. Artuso, J. Werning, C. Prync, A. Carbonetto, C. Dabsys, S. Munar, C. Salaberry, R. Berra, G. Berra, I. Fernández, N. Papouchado, M. Foti, M. Judewicz, N. Mujica, I. Muñoz, L. Alvarez, S.F. González, E. Zimmermann, J. Criscuolo, M. Melo, C. Animal biotechnology Recombinant hGH Transgenic cloned cow Biotechnology Cost effectiveness Dairy products Drug products Escherichia coli Animal biotechnology Recombinant hGH Transgenic cloned cow Hormones human growth hormone animal cell animal experiment article breed cattle clone cost effectiveness analysis cow Escherichia coli fertility fetus hematological parameters hormone synthesis milk nonhuman priority journal progeny transgenic animal Animals Animals, Genetically Modified Cattle Cloning, Organism Human Growth Hormone Humans Milk Proteins Recombinant Proteins Animalia Bos taurus Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l-1. The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone. © 2006. Fil:Barañao, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernández, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Judewicz, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681656_v124_n2_p469_Salamone |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Animal biotechnology Recombinant hGH Transgenic cloned cow Biotechnology Cost effectiveness Dairy products Drug products Escherichia coli Animal biotechnology Recombinant hGH Transgenic cloned cow Hormones human growth hormone animal cell animal experiment article breed cattle clone cost effectiveness analysis cow Escherichia coli fertility fetus hematological parameters hormone synthesis milk nonhuman priority journal progeny transgenic animal Animals Animals, Genetically Modified Cattle Cloning, Organism Human Growth Hormone Humans Milk Proteins Recombinant Proteins Animalia Bos taurus |
spellingShingle |
Animal biotechnology Recombinant hGH Transgenic cloned cow Biotechnology Cost effectiveness Dairy products Drug products Escherichia coli Animal biotechnology Recombinant hGH Transgenic cloned cow Hormones human growth hormone animal cell animal experiment article breed cattle clone cost effectiveness analysis cow Escherichia coli fertility fetus hematological parameters hormone synthesis milk nonhuman priority journal progeny transgenic animal Animals Animals, Genetically Modified Cattle Cloning, Organism Human Growth Hormone Humans Milk Proteins Recombinant Proteins Animalia Bos taurus Salamone, D. Barañao, L. Santos, C. Bussmann, L. Artuso, J. Werning, C. Prync, A. Carbonetto, C. Dabsys, S. Munar, C. Salaberry, R. Berra, G. Berra, I. Fernández, N. Papouchado, M. Foti, M. Judewicz, N. Mujica, I. Muñoz, L. Alvarez, S.F. González, E. Zimmermann, J. Criscuolo, M. Melo, C. High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
topic_facet |
Animal biotechnology Recombinant hGH Transgenic cloned cow Biotechnology Cost effectiveness Dairy products Drug products Escherichia coli Animal biotechnology Recombinant hGH Transgenic cloned cow Hormones human growth hormone animal cell animal experiment article breed cattle clone cost effectiveness analysis cow Escherichia coli fertility fetus hematological parameters hormone synthesis milk nonhuman priority journal progeny transgenic animal Animals Animals, Genetically Modified Cattle Cloning, Organism Human Growth Hormone Humans Milk Proteins Recombinant Proteins Animalia Bos taurus |
description |
Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l-1. The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone. © 2006. |
format |
JOUR |
author |
Salamone, D. Barañao, L. Santos, C. Bussmann, L. Artuso, J. Werning, C. Prync, A. Carbonetto, C. Dabsys, S. Munar, C. Salaberry, R. Berra, G. Berra, I. Fernández, N. Papouchado, M. Foti, M. Judewicz, N. Mujica, I. Muñoz, L. Alvarez, S.F. González, E. Zimmermann, J. Criscuolo, M. Melo, C. |
author_facet |
Salamone, D. Barañao, L. Santos, C. Bussmann, L. Artuso, J. Werning, C. Prync, A. Carbonetto, C. Dabsys, S. Munar, C. Salaberry, R. Berra, G. Berra, I. Fernández, N. Papouchado, M. Foti, M. Judewicz, N. Mujica, I. Muñoz, L. Alvarez, S.F. González, E. Zimmermann, J. Criscuolo, M. Melo, C. |
author_sort |
Salamone, D. |
title |
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
title_short |
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
title_full |
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
title_fullStr |
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
title_full_unstemmed |
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
title_sort |
high level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
url |
http://hdl.handle.net/20.500.12110/paper_01681656_v124_n2_p469_Salamone |
work_keys_str_mv |
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