Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress
The behaviour and state of the envelopes from B. subtilis cultures grown in Luria Bertani (LB) medium with and without 1.5 M NaCl are compared. Under hypertonic conditions, the hydrophobicity of the cultures increases. The phospholipid and fatty acid (FA) compositions show important differences: a h...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01681605_v55_n1-3_p137_Lopez |
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todo:paper_01681605_v55_n1-3_p137_Lopez2023-10-03T15:06:00Z Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress López, C.S. Heras, H. Garda, H. Ruzal, S. Sánchez-Rivas, C. Rivas, E. Bacillus subtilis Fatty acids Membranes Phospholipids Stress fatty acid membrane lipid phosphatidylglycerol phospholipid adaptation Bacillus subtilis bacterial membrane conference paper fluorescence hydrophobicity lipid analysis lipid composition membrane fluidity nonhuman osmotic stress Bacillus subtilis Cell Membrane Permeability Fatty Acids Membrane Fluidity Membrane Lipids Phospholipids Water-Electrolyte Balance Bacillus subtilis Luria The behaviour and state of the envelopes from B. subtilis cultures grown in Luria Bertani (LB) medium with and without 1.5 M NaCl are compared. Under hypertonic conditions, the hydrophobicity of the cultures increases. The phospholipid and fatty acid (FA) compositions show important differences: a higher cardiolipin (CL) content [at the expense of phosphatidylglycerol (PG)], and a higher unsaturated and straight chain FA content. The fluidity of the membranes, determined with fluorescent probes, indicates an increase in viscosity of the cytoplasmic membrane. The consequences of these variations in membrane permeability and osmotolerance are discussed. Copyright (C) 2000 Elsevier Science B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681605_v55_n1-3_p137_Lopez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bacillus subtilis Fatty acids Membranes Phospholipids Stress fatty acid membrane lipid phosphatidylglycerol phospholipid adaptation Bacillus subtilis bacterial membrane conference paper fluorescence hydrophobicity lipid analysis lipid composition membrane fluidity nonhuman osmotic stress Bacillus subtilis Cell Membrane Permeability Fatty Acids Membrane Fluidity Membrane Lipids Phospholipids Water-Electrolyte Balance Bacillus subtilis Luria |
| spellingShingle |
Bacillus subtilis Fatty acids Membranes Phospholipids Stress fatty acid membrane lipid phosphatidylglycerol phospholipid adaptation Bacillus subtilis bacterial membrane conference paper fluorescence hydrophobicity lipid analysis lipid composition membrane fluidity nonhuman osmotic stress Bacillus subtilis Cell Membrane Permeability Fatty Acids Membrane Fluidity Membrane Lipids Phospholipids Water-Electrolyte Balance Bacillus subtilis Luria López, C.S. Heras, H. Garda, H. Ruzal, S. Sánchez-Rivas, C. Rivas, E. Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress |
| topic_facet |
Bacillus subtilis Fatty acids Membranes Phospholipids Stress fatty acid membrane lipid phosphatidylglycerol phospholipid adaptation Bacillus subtilis bacterial membrane conference paper fluorescence hydrophobicity lipid analysis lipid composition membrane fluidity nonhuman osmotic stress Bacillus subtilis Cell Membrane Permeability Fatty Acids Membrane Fluidity Membrane Lipids Phospholipids Water-Electrolyte Balance Bacillus subtilis Luria |
| description |
The behaviour and state of the envelopes from B. subtilis cultures grown in Luria Bertani (LB) medium with and without 1.5 M NaCl are compared. Under hypertonic conditions, the hydrophobicity of the cultures increases. The phospholipid and fatty acid (FA) compositions show important differences: a higher cardiolipin (CL) content [at the expense of phosphatidylglycerol (PG)], and a higher unsaturated and straight chain FA content. The fluidity of the membranes, determined with fluorescent probes, indicates an increase in viscosity of the cytoplasmic membrane. The consequences of these variations in membrane permeability and osmotolerance are discussed. Copyright (C) 2000 Elsevier Science B.V. |
| format |
JOUR |
| author |
López, C.S. Heras, H. Garda, H. Ruzal, S. Sánchez-Rivas, C. Rivas, E. |
| author_facet |
López, C.S. Heras, H. Garda, H. Ruzal, S. Sánchez-Rivas, C. Rivas, E. |
| author_sort |
López, C.S. |
| title |
Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress |
| title_short |
Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress |
| title_full |
Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress |
| title_fullStr |
Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress |
| title_full_unstemmed |
Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress |
| title_sort |
biochemical and biophysical studies of bacillus subtilis envelopes under hyperosmotic stress |
| url |
http://hdl.handle.net/20.500.12110/paper_01681605_v55_n1-3_p137_Lopez |
| work_keys_str_mv |
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