Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach
Small-spored Alternaria have been isolated from a wide variety of food crops, causing both economic losses and human health risk due to the metabolites produced. Their taxonomy has been discussed widely, but no scientific consensus has been established in this field to date. Argentina is a major exp...
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todo:paper_01681605_v257_n_p206_daCruzCabral2023-10-03T15:05:54Z Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach da Cruz Cabral, L. Rodriguero, M. Stenglein, S. Fog Nielsen, K. Patriarca, A. Alternaria Endopolygalacturonase gene Food Metabolites profile Polyphasic approach Alternaria Argentina Article blueberry Capsicum annuum controlled study food crop food safety grain health hazard lowbush blueberry morphological trait nonhuman pest control phylogeny tomato Alternaria Bayes theorem black pepper classification fruit fungus spore genetics isolation and purification microbiology secondary metabolism Vaccinium vegetable wheat fungal DNA polygalacturonase Alternaria Argentina Bayes Theorem Blueberry Plants DNA, Fungal Fruit Lycopersicon esculentum Phylogeny Piper nigrum Polygalacturonase Secondary Metabolism Spores, Fungal Triticum Vegetables Small-spored Alternaria have been isolated from a wide variety of food crops, causing both economic losses and human health risk due to the metabolites produced. Their taxonomy has been discussed widely, but no scientific consensus has been established in this field to date. Argentina is a major exporter of agricultural products, so it is essential to thoroughly understand the physiological behaviour of this pathogen in a food safety context. Thus, the objective of this work was to characterize small-spored Alternaria spp. obtained from tomato fruits, pepper fruits, wheat grains and blueberries from Argentina by a polyphasic approach involving metabolomic and phylogenetic analyses based on molecular and morphological characters. Morphological analysis divided the population studied into three groups; A. arborescens sp.-grp., A. tenuissima sp.-grp., and A. alternata sp.-grp. However, when these characters were simultaneously analysed with molecular data, no clearly separated groups were obtained. Haplotype network and phylogenetic analysis (both Bayesian and maximum parsimony) of a conserved region yielded the same result, suggesting that all isolates belong to the same species. Furthermore, no correlation could be established between morphological species-groups and a metabolite or group of metabolites synthesized. Thus, the whole set of analyses carried out in the present work supports the hypothesis that these small-spored Alternaria isolates from food belong to the same species. Identification at species level through classical morphology or modern molecular techniques does not seem to be a useful tool to predict toxicological risk in food matrices. The detection of any small-spored Alternaria from Section Alternaria (D.P. Lawr., Gannibal, Peever & B.M. Pryor 2013) in food implies a potential toxicological risk. © 2017 Elsevier B.V. Fil:Rodriguero, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Patriarca, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681605_v257_n_p206_daCruzCabral |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alternaria Endopolygalacturonase gene Food Metabolites profile Polyphasic approach Alternaria Argentina Article blueberry Capsicum annuum controlled study food crop food safety grain health hazard lowbush blueberry morphological trait nonhuman pest control phylogeny tomato Alternaria Bayes theorem black pepper classification fruit fungus spore genetics isolation and purification microbiology secondary metabolism Vaccinium vegetable wheat fungal DNA polygalacturonase Alternaria Argentina Bayes Theorem Blueberry Plants DNA, Fungal Fruit Lycopersicon esculentum Phylogeny Piper nigrum Polygalacturonase Secondary Metabolism Spores, Fungal Triticum Vegetables |
| spellingShingle |
Alternaria Endopolygalacturonase gene Food Metabolites profile Polyphasic approach Alternaria Argentina Article blueberry Capsicum annuum controlled study food crop food safety grain health hazard lowbush blueberry morphological trait nonhuman pest control phylogeny tomato Alternaria Bayes theorem black pepper classification fruit fungus spore genetics isolation and purification microbiology secondary metabolism Vaccinium vegetable wheat fungal DNA polygalacturonase Alternaria Argentina Bayes Theorem Blueberry Plants DNA, Fungal Fruit Lycopersicon esculentum Phylogeny Piper nigrum Polygalacturonase Secondary Metabolism Spores, Fungal Triticum Vegetables da Cruz Cabral, L. Rodriguero, M. Stenglein, S. Fog Nielsen, K. Patriarca, A. Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach |
| topic_facet |
Alternaria Endopolygalacturonase gene Food Metabolites profile Polyphasic approach Alternaria Argentina Article blueberry Capsicum annuum controlled study food crop food safety grain health hazard lowbush blueberry morphological trait nonhuman pest control phylogeny tomato Alternaria Bayes theorem black pepper classification fruit fungus spore genetics isolation and purification microbiology secondary metabolism Vaccinium vegetable wheat fungal DNA polygalacturonase Alternaria Argentina Bayes Theorem Blueberry Plants DNA, Fungal Fruit Lycopersicon esculentum Phylogeny Piper nigrum Polygalacturonase Secondary Metabolism Spores, Fungal Triticum Vegetables |
| description |
Small-spored Alternaria have been isolated from a wide variety of food crops, causing both economic losses and human health risk due to the metabolites produced. Their taxonomy has been discussed widely, but no scientific consensus has been established in this field to date. Argentina is a major exporter of agricultural products, so it is essential to thoroughly understand the physiological behaviour of this pathogen in a food safety context. Thus, the objective of this work was to characterize small-spored Alternaria spp. obtained from tomato fruits, pepper fruits, wheat grains and blueberries from Argentina by a polyphasic approach involving metabolomic and phylogenetic analyses based on molecular and morphological characters. Morphological analysis divided the population studied into three groups; A. arborescens sp.-grp., A. tenuissima sp.-grp., and A. alternata sp.-grp. However, when these characters were simultaneously analysed with molecular data, no clearly separated groups were obtained. Haplotype network and phylogenetic analysis (both Bayesian and maximum parsimony) of a conserved region yielded the same result, suggesting that all isolates belong to the same species. Furthermore, no correlation could be established between morphological species-groups and a metabolite or group of metabolites synthesized. Thus, the whole set of analyses carried out in the present work supports the hypothesis that these small-spored Alternaria isolates from food belong to the same species. Identification at species level through classical morphology or modern molecular techniques does not seem to be a useful tool to predict toxicological risk in food matrices. The detection of any small-spored Alternaria from Section Alternaria (D.P. Lawr., Gannibal, Peever & B.M. Pryor 2013) in food implies a potential toxicological risk. © 2017 Elsevier B.V. |
| format |
JOUR |
| author |
da Cruz Cabral, L. Rodriguero, M. Stenglein, S. Fog Nielsen, K. Patriarca, A. |
| author_facet |
da Cruz Cabral, L. Rodriguero, M. Stenglein, S. Fog Nielsen, K. Patriarca, A. |
| author_sort |
da Cruz Cabral, L. |
| title |
Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach |
| title_short |
Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach |
| title_full |
Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach |
| title_fullStr |
Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach |
| title_full_unstemmed |
Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach |
| title_sort |
characterization of small-spored alternaria from argentinean crops through a polyphasic approach |
| url |
http://hdl.handle.net/20.500.12110/paper_01681605_v257_n_p206_daCruzCabral |
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