Improved biovolume estimation of Microcystis aeruginosa colonies: A statistical approach

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The Microcystis aeruginosa complex (MAC) clusters many of the most common freshwater and brackish bloom-forming cyanobacteria. In monitoring protocols, biovolume estimation is a common approach to determine MAC colonies biomass and useful for prediction purposes. Biovolume (μm3 mL−1) is calculated m...

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Autores principales:	Alcántara, I., Piccini, C., Segura, A.M., Deus, S., González, C., Martínez de la Escalera, G., Kruk, C.
Formato:	JOUR
Materias:	Cyanobacteria monitoring Harmful algal blooms Machine learning Phytoplankton counting brackish water fresh water sea water Article autumn bacterium colony bacterium detection biomass environmental monitoring Microcystis aeruginosa nonhuman priority journal seasonal variation spring statistical model winter
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01677012_v151_n_p20_Alcantara
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01677012_v151_n_p20_Alcantara
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spelling	todo:paper_01677012_v151_n_p20_Alcantara2023-10-03T15:05:10Z Improved biovolume estimation of Microcystis aeruginosa colonies: A statistical approach Alcántara, I. Piccini, C. Segura, A.M. Deus, S. González, C. Martínez de la Escalera, G. Kruk, C. Cyanobacteria monitoring Harmful algal blooms Machine learning Phytoplankton counting brackish water fresh water sea water Article autumn bacterium colony bacterium detection biomass environmental monitoring Microcystis aeruginosa nonhuman priority journal seasonal variation spring statistical model winter The Microcystis aeruginosa complex (MAC) clusters many of the most common freshwater and brackish bloom-forming cyanobacteria. In monitoring protocols, biovolume estimation is a common approach to determine MAC colonies biomass and useful for prediction purposes. Biovolume (μm3 mL−1) is calculated multiplying organism abundance (orgL−1) by colonial volume (μm3org−1). Colonial volume is estimated based on geometric shapes and requires accurate measurements of dimensions using optical microscopy. A trade-off between easy-to-measure but low-accuracy simple shapes (e.g. sphere) and time costly but high-accuracy complex shapes (e.g. ellipsoid) volume estimation is posed. Overestimations effects in ecological studies and management decisions associated to harmful blooms are significant due to the large sizes of MAC colonies. In this work, we aimed to increase the precision of MAC biovolume estimations by developing a statistical model based on two easy-to-measure dimensions. We analyzed field data from a wide environmental gradient (800 km) spanning freshwater to estuarine and seawater. We measured length, width and depth from ca. 5700 colonies under an inverted microscope and estimated colonial volume using three different recommended geometrical shapes (sphere, prolate spheroid and ellipsoid). Because of the non-spherical shape of MAC the ellipsoid resulted in the most accurate approximation, whereas the sphere overestimated colonial volume (3–80) especially for large colonies (MLD higher than 300 μm). Ellipsoid requires measuring three dimensions and is time-consuming. Therefore, we constructed different statistical models to predict organisms depth based on length and width. Splitting the data into training (2/3) and test (1/3) sets, all models resulted in low training (1.41–1.44%) and testing average error (1.3–2.0%). The models were also evaluated using three other independent datasets. The multiple linear model was finally selected to calculate MAC volume as an ellipsoid based on length and width. This work contributes to achieve a better estimation of MAC volume applicable to monitoring programs as well as to ecological research. © 2018 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01677012_v151_n_p20_Alcantara
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cyanobacteria monitoring Harmful algal blooms Machine learning Phytoplankton counting brackish water fresh water sea water Article autumn bacterium colony bacterium detection biomass environmental monitoring Microcystis aeruginosa nonhuman priority journal seasonal variation spring statistical model winter
spellingShingle	Cyanobacteria monitoring Harmful algal blooms Machine learning Phytoplankton counting brackish water fresh water sea water Article autumn bacterium colony bacterium detection biomass environmental monitoring Microcystis aeruginosa nonhuman priority journal seasonal variation spring statistical model winter Alcántara, I. Piccini, C. Segura, A.M. Deus, S. González, C. Martínez de la Escalera, G. Kruk, C. Improved biovolume estimation of Microcystis aeruginosa colonies: A statistical approach
topic_facet	Cyanobacteria monitoring Harmful algal blooms Machine learning Phytoplankton counting brackish water fresh water sea water Article autumn bacterium colony bacterium detection biomass environmental monitoring Microcystis aeruginosa nonhuman priority journal seasonal variation spring statistical model winter
description	The Microcystis aeruginosa complex (MAC) clusters many of the most common freshwater and brackish bloom-forming cyanobacteria. In monitoring protocols, biovolume estimation is a common approach to determine MAC colonies biomass and useful for prediction purposes. Biovolume (μm3 mL−1) is calculated multiplying organism abundance (orgL−1) by colonial volume (μm3org−1). Colonial volume is estimated based on geometric shapes and requires accurate measurements of dimensions using optical microscopy. A trade-off between easy-to-measure but low-accuracy simple shapes (e.g. sphere) and time costly but high-accuracy complex shapes (e.g. ellipsoid) volume estimation is posed. Overestimations effects in ecological studies and management decisions associated to harmful blooms are significant due to the large sizes of MAC colonies. In this work, we aimed to increase the precision of MAC biovolume estimations by developing a statistical model based on two easy-to-measure dimensions. We analyzed field data from a wide environmental gradient (800 km) spanning freshwater to estuarine and seawater. We measured length, width and depth from ca. 5700 colonies under an inverted microscope and estimated colonial volume using three different recommended geometrical shapes (sphere, prolate spheroid and ellipsoid). Because of the non-spherical shape of MAC the ellipsoid resulted in the most accurate approximation, whereas the sphere overestimated colonial volume (3–80) especially for large colonies (MLD higher than 300 μm). Ellipsoid requires measuring three dimensions and is time-consuming. Therefore, we constructed different statistical models to predict organisms depth based on length and width. Splitting the data into training (2/3) and test (1/3) sets, all models resulted in low training (1.41–1.44%) and testing average error (1.3–2.0%). The models were also evaluated using three other independent datasets. The multiple linear model was finally selected to calculate MAC volume as an ellipsoid based on length and width. This work contributes to achieve a better estimation of MAC volume applicable to monitoring programs as well as to ecological research. © 2018 Elsevier B.V.
format	JOUR
author	Alcántara, I. Piccini, C. Segura, A.M. Deus, S. González, C. Martínez de la Escalera, G. Kruk, C.
author_facet	Alcántara, I. Piccini, C. Segura, A.M. Deus, S. González, C. Martínez de la Escalera, G. Kruk, C.
author_sort	Alcántara, I.
title	Improved biovolume estimation of Microcystis aeruginosa colonies: A statistical approach
title_short	Improved biovolume estimation of Microcystis aeruginosa colonies: A statistical approach
title_full	Improved biovolume estimation of Microcystis aeruginosa colonies: A statistical approach
title_fullStr	Improved biovolume estimation of Microcystis aeruginosa colonies: A statistical approach
title_full_unstemmed	Improved biovolume estimation of Microcystis aeruginosa colonies: A statistical approach
title_sort	improved biovolume estimation of microcystis aeruginosa colonies: a statistical approach
url	http://hdl.handle.net/20.500.12110/paper_01677012_v151_n_p20_Alcantara
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Improved biovolume estimation of Microcystis aeruginosa colonies: A statistical approach

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