Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum
Italian ryegrass (Lolium multiflorum) is an annual grass considered as one of the most important temperate forage grasses in the world. However, it is recalcitrant to plant tissue culture techniques hindering its genetic manipulation. Epichloë occultans is an endophytic fungus associated with L. mul...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01676857_v130_n1_p37_Regalado |
| Aporte de: |
| id |
todo:paper_01676857_v130_n1_p37_Regalado |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_01676857_v130_n1_p37_Regalado2023-10-03T15:05:07Z Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum Regalado, J.J. Vignale, M.V. Novas, M.V. Pitta-Alvarez, S.I. Iannone, L.J. Callus culture Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Nucleic acids Plants (botany) Tissue culture Callus cultures Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Seed Efficiency Fungi Plants Reforestation Italian ryegrass (Lolium multiflorum) is an annual grass considered as one of the most important temperate forage grasses in the world. However, it is recalcitrant to plant tissue culture techniques hindering its genetic manipulation. Epichloë occultans is an endophytic fungus associated with L. multiflorum. This symbiosis causes improvements in physiological and ecological traits of the host plants. The objective of this work was to study the effect of E. occultans on L. multiflorum micropropagation. We compared the response of endophyte-infected (E+) and endophyte-free (E−) seeds in different micropropagation stages. The E+ seeds were more successful than E− seeds in in vitro germination (83 ± 5 vs. 63 ± 6%), callus induction (78 ± 5 vs. 57 ± 6%), callus proliferation (average diameter of 21.5 ± 1.3 mm in two subcultures vs 17.3 ± 0.8 mm in three subcultures) and plant regeneration from callus (83 ± 7 vs. 30 ± 8%). These results indicate that E. occultans enhances significantly L. multiflorum micropropagation. The use of endophyte-infected (E+) seeds can be a solution to make this grass more amenable to different biotechnological tools, such as the genetic transformation. © 2017, Springer Science+Business Media Dordrecht. Fil:Novas, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iannone, L.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01676857_v130_n1_p37_Regalado |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Callus culture Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Nucleic acids Plants (botany) Tissue culture Callus cultures Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Seed Efficiency Fungi Plants Reforestation |
| spellingShingle |
Callus culture Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Nucleic acids Plants (botany) Tissue culture Callus cultures Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Seed Efficiency Fungi Plants Reforestation Regalado, J.J. Vignale, M.V. Novas, M.V. Pitta-Alvarez, S.I. Iannone, L.J. Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum |
| topic_facet |
Callus culture Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Nucleic acids Plants (botany) Tissue culture Callus cultures Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Seed Efficiency Fungi Plants Reforestation |
| description |
Italian ryegrass (Lolium multiflorum) is an annual grass considered as one of the most important temperate forage grasses in the world. However, it is recalcitrant to plant tissue culture techniques hindering its genetic manipulation. Epichloë occultans is an endophytic fungus associated with L. multiflorum. This symbiosis causes improvements in physiological and ecological traits of the host plants. The objective of this work was to study the effect of E. occultans on L. multiflorum micropropagation. We compared the response of endophyte-infected (E+) and endophyte-free (E−) seeds in different micropropagation stages. The E+ seeds were more successful than E− seeds in in vitro germination (83 ± 5 vs. 63 ± 6%), callus induction (78 ± 5 vs. 57 ± 6%), callus proliferation (average diameter of 21.5 ± 1.3 mm in two subcultures vs 17.3 ± 0.8 mm in three subcultures) and plant regeneration from callus (83 ± 7 vs. 30 ± 8%). These results indicate that E. occultans enhances significantly L. multiflorum micropropagation. The use of endophyte-infected (E+) seeds can be a solution to make this grass more amenable to different biotechnological tools, such as the genetic transformation. © 2017, Springer Science+Business Media Dordrecht. |
| format |
JOUR |
| author |
Regalado, J.J. Vignale, M.V. Novas, M.V. Pitta-Alvarez, S.I. Iannone, L.J. |
| author_facet |
Regalado, J.J. Vignale, M.V. Novas, M.V. Pitta-Alvarez, S.I. Iannone, L.J. |
| author_sort |
Regalado, J.J. |
| title |
Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum |
| title_short |
Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum |
| title_full |
Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum |
| title_fullStr |
Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum |
| title_full_unstemmed |
Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum |
| title_sort |
epichloë occultans enhances micropropagation efficiency in lolium multiflorum |
| url |
http://hdl.handle.net/20.500.12110/paper_01676857_v130_n1_p37_Regalado |
| work_keys_str_mv |
AT regaladojj epichloeoccultansenhancesmicropropagationefficiencyinloliummultiflorum AT vignalemv epichloeoccultansenhancesmicropropagationefficiencyinloliummultiflorum AT novasmv epichloeoccultansenhancesmicropropagationefficiencyinloliummultiflorum AT pittaalvarezsi epichloeoccultansenhancesmicropropagationefficiencyinloliummultiflorum AT iannonelj epichloeoccultansenhancesmicropropagationefficiencyinloliummultiflorum |
| _version_ |
1807315936821116928 |