A new P. putida instrumental toxicity bioassay

Here, we present a new toxicity bioassay (CO<inf>2</inf>-TOX), able to detect toxic or inhibitory compounds in water samples, based on the quantification of Pseudomonas putida KT2440 CO<inf>2</inf> production. The metabolically produced CO<inf>2</inf> was measured...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Figueredo, F., Abrevaya, X.C., Cortón, E.
Formato:	JOUR
Materias:	Acute toxicity CO<inf>2</inf> potentiometric electrode Lyophilized bacteria Respirometry Bacteria Copper Electrodes Health hazards Heavy metals Lead Mixtures Potentiometers (electric measuring instruments) Toxicity Zinc 3 ,5-dichlorophenol Industrial wastewaters Inhibitory compounds Low concentrations Optimization studies Potentiometric electrode Bioassay 3,5 dichlorophenol arsenic carbon dioxide copper ion lead oxygen surface water water zinc ion 3,5-dichlorophenol chlorophenol dangerous goods phenol phenol derivative waste water water pollutant bioassay detection method electrode heavy metal inhibitor metabolism microbial activity optimization quantitative analysis toxicity Article breathing rate carbon dioxide breathing carbon dioxide toxicity bioassay chemical binding concentration response controlled study cost effectiveness analysis EC50 incubation time lyophilisate metal mixture nonhuman pH process optimization Pseudomonas cell multiplication inhibition test Pseudomonas putida sensitivity analysis standardization survival rate toxicity testing turbidity waste water management drug effects environmental monitoring industry potentiometry procedures Biological Assay Chlorophenols Environmental Monitoring Hazardous Substances Industry Oxygen Phenol Phenols Potentiometry Toxicity Tests Waste Water Water Pollutants, Chemical
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01676369_v187_n5_p_Figueredo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01676369_v187_n5_p_Figueredo
record_format	dspace
spelling	todo:paper_01676369_v187_n5_p_Figueredo2023-10-03T15:05:00Z A new P. putida instrumental toxicity bioassay Figueredo, F. Abrevaya, X.C. Cortón, E. Acute toxicity CO<inf>2</inf> potentiometric electrode Lyophilized bacteria Respirometry Bacteria Copper Electrodes Health hazards Heavy metals Lead Mixtures Potentiometers (electric measuring instruments) Toxicity Zinc 3 ,5-dichlorophenol Acute toxicity Industrial wastewaters Inhibitory compounds Low concentrations Optimization studies Potentiometric electrode Respirometry Bioassay 3,5 dichlorophenol arsenic carbon dioxide copper ion lead oxygen surface water water zinc ion 3,5-dichlorophenol chlorophenol dangerous goods phenol phenol derivative waste water water pollutant bioassay detection method electrode heavy metal inhibitor metabolism microbial activity optimization quantitative analysis toxicity Article breathing rate carbon dioxide breathing carbon dioxide toxicity bioassay chemical binding concentration response controlled study cost effectiveness analysis EC50 incubation time lyophilisate metal mixture nonhuman pH process optimization Pseudomonas cell multiplication inhibition test Pseudomonas putida sensitivity analysis standardization survival rate toxicity testing turbidity waste water management bioassay dangerous goods drug effects electrode environmental monitoring industry potentiometry procedures Pseudomonas putida toxicity toxicity testing waste water water pollutant Pseudomonas putida Biological Assay Chlorophenols Electrodes Environmental Monitoring Hazardous Substances Industry Oxygen Phenol Phenols Potentiometry Pseudomonas putida Toxicity Tests Waste Water Water Pollutants, Chemical Here, we present a new toxicity bioassay (CO<inf>2</inf>-TOX), able to detect toxic or inhibitory compounds in water samples, based on the quantification of Pseudomonas putida KT2440 CO<inf>2</inf> production. The metabolically produced CO<inf>2</inf> was measured continuously and directly in the liquid assay media, with a potentiometric gas electrode. The optimization studies were performed using as a model toxicant 3,5-DCP (3,5-dichlorophenol); later, heavy metals (Pb2+, Cu2+, or Zn2+) and a metalloid (As5+) were assayed. The response to toxics was evident after 15 min of incubation and at relatively low concentrations (e.g., 1.1 mg/L of 3,5-DCP), showing that the CO<inf>2</inf>-TOX bioassay is fast and sensitive. The EC<inf>50</inf> values obtained were 4.93, 0.12, 6.05, 32.17, and 37.81 mg/L for 3,5-DCP, Cu2+, Zn2+, As5+, and Pb2+, respectively, at neutral pH. Additionally, the effect of the pH of the sample and the use of lyophilized bacteria were also analyzed showing that the bioassay can be implemented in different conditions. Moreover, highly turbid samples and samples with very low oxygen levels were measured successfully with the new instrumental bioassay described here. Finally, simulated samples containing 3,5-DCP or a heavy metal mixture were tested using the proposed bioassay and a standard ISO bioassay, showing that our test is more sensible to the phenol but less sensible to the metal mixtures. Therefore, we propose CO<inf>2</inf>-TOX as a rapid, sensitive, low-cost, and robust instrumental bioassay that could perform as an industrial wastewater-process monitor among other applications. © 2015, Springer International Publishing Switzerland. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01676369_v187_n5_p_Figueredo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Acute toxicity CO<inf>2</inf> potentiometric electrode Lyophilized bacteria Respirometry Bacteria Copper Electrodes Health hazards Heavy metals Lead Mixtures Potentiometers (electric measuring instruments) Toxicity Zinc 3 ,5-dichlorophenol Acute toxicity Industrial wastewaters Inhibitory compounds Low concentrations Optimization studies Potentiometric electrode Respirometry Bioassay 3,5 dichlorophenol arsenic carbon dioxide copper ion lead oxygen surface water water zinc ion 3,5-dichlorophenol chlorophenol dangerous goods phenol phenol derivative waste water water pollutant bioassay detection method electrode heavy metal inhibitor metabolism microbial activity optimization quantitative analysis toxicity Article breathing rate carbon dioxide breathing carbon dioxide toxicity bioassay chemical binding concentration response controlled study cost effectiveness analysis EC50 incubation time lyophilisate metal mixture nonhuman pH process optimization Pseudomonas cell multiplication inhibition test Pseudomonas putida sensitivity analysis standardization survival rate toxicity testing turbidity waste water management bioassay dangerous goods drug effects electrode environmental monitoring industry potentiometry procedures Pseudomonas putida toxicity toxicity testing waste water water pollutant Pseudomonas putida Biological Assay Chlorophenols Electrodes Environmental Monitoring Hazardous Substances Industry Oxygen Phenol Phenols Potentiometry Pseudomonas putida Toxicity Tests Waste Water Water Pollutants, Chemical
spellingShingle	Acute toxicity CO<inf>2</inf> potentiometric electrode Lyophilized bacteria Respirometry Bacteria Copper Electrodes Health hazards Heavy metals Lead Mixtures Potentiometers (electric measuring instruments) Toxicity Zinc 3 ,5-dichlorophenol Acute toxicity Industrial wastewaters Inhibitory compounds Low concentrations Optimization studies Potentiometric electrode Respirometry Bioassay 3,5 dichlorophenol arsenic carbon dioxide copper ion lead oxygen surface water water zinc ion 3,5-dichlorophenol chlorophenol dangerous goods phenol phenol derivative waste water water pollutant bioassay detection method electrode heavy metal inhibitor metabolism microbial activity optimization quantitative analysis toxicity Article breathing rate carbon dioxide breathing carbon dioxide toxicity bioassay chemical binding concentration response controlled study cost effectiveness analysis EC50 incubation time lyophilisate metal mixture nonhuman pH process optimization Pseudomonas cell multiplication inhibition test Pseudomonas putida sensitivity analysis standardization survival rate toxicity testing turbidity waste water management bioassay dangerous goods drug effects electrode environmental monitoring industry potentiometry procedures Pseudomonas putida toxicity toxicity testing waste water water pollutant Pseudomonas putida Biological Assay Chlorophenols Electrodes Environmental Monitoring Hazardous Substances Industry Oxygen Phenol Phenols Potentiometry Pseudomonas putida Toxicity Tests Waste Water Water Pollutants, Chemical Figueredo, F. Abrevaya, X.C. Cortón, E. A new P. putida instrumental toxicity bioassay
topic_facet	Acute toxicity CO<inf>2</inf> potentiometric electrode Lyophilized bacteria Respirometry Bacteria Copper Electrodes Health hazards Heavy metals Lead Mixtures Potentiometers (electric measuring instruments) Toxicity Zinc 3 ,5-dichlorophenol Acute toxicity Industrial wastewaters Inhibitory compounds Low concentrations Optimization studies Potentiometric electrode Respirometry Bioassay 3,5 dichlorophenol arsenic carbon dioxide copper ion lead oxygen surface water water zinc ion 3,5-dichlorophenol chlorophenol dangerous goods phenol phenol derivative waste water water pollutant bioassay detection method electrode heavy metal inhibitor metabolism microbial activity optimization quantitative analysis toxicity Article breathing rate carbon dioxide breathing carbon dioxide toxicity bioassay chemical binding concentration response controlled study cost effectiveness analysis EC50 incubation time lyophilisate metal mixture nonhuman pH process optimization Pseudomonas cell multiplication inhibition test Pseudomonas putida sensitivity analysis standardization survival rate toxicity testing turbidity waste water management bioassay dangerous goods drug effects electrode environmental monitoring industry potentiometry procedures Pseudomonas putida toxicity toxicity testing waste water water pollutant Pseudomonas putida Biological Assay Chlorophenols Electrodes Environmental Monitoring Hazardous Substances Industry Oxygen Phenol Phenols Potentiometry Pseudomonas putida Toxicity Tests Waste Water Water Pollutants, Chemical
description	Here, we present a new toxicity bioassay (CO<inf>2</inf>-TOX), able to detect toxic or inhibitory compounds in water samples, based on the quantification of Pseudomonas putida KT2440 CO<inf>2</inf> production. The metabolically produced CO<inf>2</inf> was measured continuously and directly in the liquid assay media, with a potentiometric gas electrode. The optimization studies were performed using as a model toxicant 3,5-DCP (3,5-dichlorophenol); later, heavy metals (Pb2+, Cu2+, or Zn2+) and a metalloid (As5+) were assayed. The response to toxics was evident after 15 min of incubation and at relatively low concentrations (e.g., 1.1 mg/L of 3,5-DCP), showing that the CO<inf>2</inf>-TOX bioassay is fast and sensitive. The EC<inf>50</inf> values obtained were 4.93, 0.12, 6.05, 32.17, and 37.81 mg/L for 3,5-DCP, Cu2+, Zn2+, As5+, and Pb2+, respectively, at neutral pH. Additionally, the effect of the pH of the sample and the use of lyophilized bacteria were also analyzed showing that the bioassay can be implemented in different conditions. Moreover, highly turbid samples and samples with very low oxygen levels were measured successfully with the new instrumental bioassay described here. Finally, simulated samples containing 3,5-DCP or a heavy metal mixture were tested using the proposed bioassay and a standard ISO bioassay, showing that our test is more sensible to the phenol but less sensible to the metal mixtures. Therefore, we propose CO<inf>2</inf>-TOX as a rapid, sensitive, low-cost, and robust instrumental bioassay that could perform as an industrial wastewater-process monitor among other applications. © 2015, Springer International Publishing Switzerland.
format	JOUR
author	Figueredo, F. Abrevaya, X.C. Cortón, E.
author_facet	Figueredo, F. Abrevaya, X.C. Cortón, E.
author_sort	Figueredo, F.
title	A new P. putida instrumental toxicity bioassay
title_short	A new P. putida instrumental toxicity bioassay
title_full	A new P. putida instrumental toxicity bioassay
title_fullStr	A new P. putida instrumental toxicity bioassay
title_full_unstemmed	A new P. putida instrumental toxicity bioassay
title_sort	new p. putida instrumental toxicity bioassay
url	http://hdl.handle.net/20.500.12110/paper_01676369_v187_n5_p_Figueredo
work_keys_str_mv	AT figueredof anewpputidainstrumentaltoxicitybioassay AT abrevayaxc anewpputidainstrumentaltoxicitybioassay AT cortone anewpputidainstrumentaltoxicitybioassay AT figueredof newpputidainstrumentaltoxicitybioassay AT abrevayaxc newpputidainstrumentaltoxicitybioassay AT cortone newpputidainstrumentaltoxicitybioassay
_version_	1807319788387565568

A new P. putida instrumental toxicity bioassay

Ejemplares similares