Studies on regulatory mechanisms of heme biosynthesis in hepatocytes from experimental-diabetic rats

Isolated hepatocytes from rats with experimental diabetes exhibit increased content of cytochrome P-450 and cyclic AMP and normal activities of the regulatory enzymes δ-aminolevulinic acid synthase and ferrochelatase. The inducing effect exerted by phenobarbital on cytochrome P-450, δ-aminolevulinic...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Cánepa, E.T., Galvagno, M.A., Llambías, E.B.C., Passeron, S., Grinstein, M.
Formato: JOUR
Materias:
(Diabetic rat hepatocyte)
cyclic AMP
Cytochrome P-450
Enzyme induction
Ferrochelatase
δ-Aminolevulinic acid synthase
5 aminolevulinate synthase
cyclic amp
cytochrome p450
ferrochelatase
heme
animal model
biosynthesis
diabetes mellitus
endocrine system
etiology
experimental diabetes mellitus
liver
liver cell
nonhuman
priority journal
rat
5-Aminolevulinate Synthetase
Animals
Bucladesine
Cyclic AMP
Cytochrome P-450 Enzyme System
Diabetes Mellitus, Experimental
Glucose
Heme
Homeostasis
Kinetics
Liver
Male
Phenobarbital
Rats
Rats, Inbred Strains
Reference Values
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01674889_v847_n2_p191_Canepa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01674889_v847_n2_p191_Canepa
record_format dspace
spelling todo:paper_01674889_v847_n2_p191_Canepa2023-10-03T15:04:53Z Studies on regulatory mechanisms of heme biosynthesis in hepatocytes from experimental-diabetic rats Cánepa, E.T. Galvagno, M.A. Llambías, E.B.C. Passeron, S. Grinstein, M. (Diabetic rat hepatocyte) cyclic AMP Cytochrome P-450 Enzyme induction Ferrochelatase δ-Aminolevulinic acid synthase 5 aminolevulinate synthase cyclic amp cytochrome p450 ferrochelatase heme animal model biosynthesis diabetes mellitus endocrine system etiology experimental diabetes mellitus liver liver cell nonhuman priority journal rat 5-Aminolevulinate Synthetase Animals Bucladesine Cyclic AMP Cytochrome P-450 Enzyme System Diabetes Mellitus, Experimental Ferrochelatase Glucose Heme Homeostasis Kinetics Liver Male Phenobarbital Rats Rats, Inbred Strains Reference Values Isolated hepatocytes from rats with experimental diabetes exhibit increased content of cytochrome P-450 and cyclic AMP and normal activities of the regulatory enzymes δ-aminolevulinic acid synthase and ferrochelatase. The inducing effect exerted by phenobarbital on cytochrome P-450, δ-aminolevulinic acid synthase and ferrochelatase biosynthesis and cyclic AMP content in diabetic hepatic cells is markedly greater than that observed in normal hepatocytes. This stimulatory response is neither enhanced by added dibutyryl cyclic AMP nor repressed by glucose. The present results suggest that the heme pathway of diabetic hepatocytes is more susceptible to porphyrinogenic factors. © 1985. Fil:Cánepa, E.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Galvagno, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Llambías, E.B.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01674889_v847_n2_p191_Canepa
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic (Diabetic rat hepatocyte)
cyclic AMP
Cytochrome P-450
Enzyme induction
Ferrochelatase
δ-Aminolevulinic acid synthase
5 aminolevulinate synthase
cyclic amp
cytochrome p450
ferrochelatase
heme
animal model
biosynthesis
diabetes mellitus
endocrine system
etiology
experimental diabetes mellitus
liver
liver cell
nonhuman
priority journal
rat
5-Aminolevulinate Synthetase
Animals
Bucladesine
Cyclic AMP
Cytochrome P-450 Enzyme System
Diabetes Mellitus, Experimental
Ferrochelatase
Glucose
Heme
Homeostasis
Kinetics
Liver
Male
Phenobarbital
Rats
Rats, Inbred Strains
Reference Values
spellingShingle (Diabetic rat hepatocyte)
cyclic AMP
Cytochrome P-450
Enzyme induction
Ferrochelatase
δ-Aminolevulinic acid synthase
5 aminolevulinate synthase
cyclic amp
cytochrome p450
ferrochelatase
heme
animal model
biosynthesis
diabetes mellitus
endocrine system
etiology
experimental diabetes mellitus
liver
liver cell
nonhuman
priority journal
rat
5-Aminolevulinate Synthetase
Animals
Bucladesine
Cyclic AMP
Cytochrome P-450 Enzyme System
Diabetes Mellitus, Experimental
Ferrochelatase
Glucose
Heme
Homeostasis
Kinetics
Liver
Male
Phenobarbital
Rats
Rats, Inbred Strains
Reference Values
Cánepa, E.T.
Galvagno, M.A.
Llambías, E.B.C.
Passeron, S.
Grinstein, M.
Studies on regulatory mechanisms of heme biosynthesis in hepatocytes from experimental-diabetic rats
topic_facet (Diabetic rat hepatocyte)
cyclic AMP
Cytochrome P-450
Enzyme induction
Ferrochelatase
δ-Aminolevulinic acid synthase
5 aminolevulinate synthase
cyclic amp
cytochrome p450
ferrochelatase
heme
animal model
biosynthesis
diabetes mellitus
endocrine system
etiology
experimental diabetes mellitus
liver
liver cell
nonhuman
priority journal
rat
5-Aminolevulinate Synthetase
Animals
Bucladesine
Cyclic AMP
Cytochrome P-450 Enzyme System
Diabetes Mellitus, Experimental
Ferrochelatase
Glucose
Heme
Homeostasis
Kinetics
Liver
Male
Phenobarbital
Rats
Rats, Inbred Strains
Reference Values
description Isolated hepatocytes from rats with experimental diabetes exhibit increased content of cytochrome P-450 and cyclic AMP and normal activities of the regulatory enzymes δ-aminolevulinic acid synthase and ferrochelatase. The inducing effect exerted by phenobarbital on cytochrome P-450, δ-aminolevulinic acid synthase and ferrochelatase biosynthesis and cyclic AMP content in diabetic hepatic cells is markedly greater than that observed in normal hepatocytes. This stimulatory response is neither enhanced by added dibutyryl cyclic AMP nor repressed by glucose. The present results suggest that the heme pathway of diabetic hepatocytes is more susceptible to porphyrinogenic factors. © 1985.
format JOUR
author Cánepa, E.T.
Galvagno, M.A.
Llambías, E.B.C.
Passeron, S.
Grinstein, M.
author_facet Cánepa, E.T.
Galvagno, M.A.
Llambías, E.B.C.
Passeron, S.
Grinstein, M.
author_sort Cánepa, E.T.
title Studies on regulatory mechanisms of heme biosynthesis in hepatocytes from experimental-diabetic rats
title_short Studies on regulatory mechanisms of heme biosynthesis in hepatocytes from experimental-diabetic rats
title_full Studies on regulatory mechanisms of heme biosynthesis in hepatocytes from experimental-diabetic rats
title_fullStr Studies on regulatory mechanisms of heme biosynthesis in hepatocytes from experimental-diabetic rats
title_full_unstemmed Studies on regulatory mechanisms of heme biosynthesis in hepatocytes from experimental-diabetic rats
title_sort studies on regulatory mechanisms of heme biosynthesis in hepatocytes from experimental-diabetic rats
url http://hdl.handle.net/20.500.12110/paper_01674889_v847_n2_p191_Canepa
work_keys_str_mv AT canepaet studiesonregulatorymechanismsofhemebiosynthesisinhepatocytesfromexperimentaldiabeticrats
AT galvagnoma studiesonregulatorymechanismsofhemebiosynthesisinhepatocytesfromexperimentaldiabeticrats
AT llambiasebc studiesonregulatorymechanismsofhemebiosynthesisinhepatocytesfromexperimentaldiabeticrats
AT passerons studiesonregulatorymechanismsofhemebiosynthesisinhepatocytesfromexperimentaldiabeticrats
AT grinsteinm studiesonregulatorymechanismsofhemebiosynthesisinhepatocytesfromexperimentaldiabeticrats
_version_ 1807320060014886912

Ejemplares similares