Regulation of extracellular ATP of human erythrocytes treated with α-hemolysin. Effects of cell volume, morphology, rheology and hemolysis
Alpha-hemolysin (HlyA) of uropathogenic strains of Escherichia coli irreversibly binds to human erythrocytes (RBCs) and triggers activation of ATP release and metabolic changes ultimately leading to hemolysis. We studied the regulation of extracellular ATP (ATPe) of RBCs exposed to HlyA. Luminometry...
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todo:paper_01674889_v1866_n5_p896_LealDenis2023-10-03T15:04:52Z Regulation of extracellular ATP of human erythrocytes treated with α-hemolysin. Effects of cell volume, morphology, rheology and hemolysis Leal Denis, M.F. Lefevre, S.D. Alvarez, C.L. Lauri, N. Enrique, N. Rinaldi, D.E. Gonzalez-Lebrero, R. Vecchio, L.E. Espelt, M.V. Stringa, P. Muñoz-Garay, C. Milesi, V. Ostuni, M.A. Herlax, V. Schwarzbaum, P.J. Adhesion, aggregation ATP-transport E.coli hemolysin Hemolysis adenosine triphosphatase alpha hemolysin apyrase calcium carbenoxolone hemoglobin liposome purinergic P2X receptor animal experiment Article calcium cell level cell aggregation cell isolation cell shape cell structure cell swelling cell volume erythrocyte flow kinetics hemolysis hemolysis assay human human cell hydrolysis impedance male mathematical model membrane potential mesenteric arterial bed nonhuman osmotic stress priority journal protein purification protein secretion rat refraction index regulatory mechanism scanning electron microscopy shear stress Alpha-hemolysin (HlyA) of uropathogenic strains of Escherichia coli irreversibly binds to human erythrocytes (RBCs) and triggers activation of ATP release and metabolic changes ultimately leading to hemolysis. We studied the regulation of extracellular ATP (ATPe) of RBCs exposed to HlyA. Luminometry was used to assess ATP release and ATPe hydrolysis, whereas changes in cell volume and morphology were determined by electrical impedance, ektacytometry and aggregometry. Exposure of RBCs to HlyA induced a strong increase of [ATPe] (3–36-fold) and hemolysis (1–44-fold), partially compensated by [ATPe] hydrolysis by ectoATPases and intracellular ATPases released by dead cells. Carbenoxolone, a pannexin 1 inhibitor, partially inhibited ATP release (43–67%). The un-acylated toxin ProHlyA and the deletion analog HlyA∆914-936 were unable to induce ATP release or hemolysis. For HlyA treated RBCs, a data driven mathematical model showed that simultaneous lytic and non-lytic release mainly governed ATPe kinetics, while ATPe hydrolysis became important after prolonged toxin exposure. HlyA induced a 1.5-fold swelling, while blocking this swelling reduced ATP release by 77%. Blocking ATPe activation of purinergic P2X receptors reduced swelling by 60–80%. HlyA-RBCs showed an acute 1.3–2.2-fold increase of Ca 2+ i, increased crenation and externalization of phosphatidylserine. Perfusion of HlyA-RBCs through adhesion platforms showed strong adhesion to activated HMEC cells, followed by rapid detachment. HlyA exposed RBCs exhibited increased sphericity under osmotic stress, reduced elongation under shear stress, and very low aggregation in viscous media. Overall results showed that HlyA-RBCs displayed activated ATP release, high but weak adhesivity, low deformability and aggregability and high sphericity. © 2019 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01674889_v1866_n5_p896_LealDenis |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adhesion, aggregation ATP-transport E.coli hemolysin Hemolysis adenosine triphosphatase alpha hemolysin apyrase calcium carbenoxolone hemoglobin liposome purinergic P2X receptor animal experiment Article calcium cell level cell aggregation cell isolation cell shape cell structure cell swelling cell volume erythrocyte flow kinetics hemolysis hemolysis assay human human cell hydrolysis impedance male mathematical model membrane potential mesenteric arterial bed nonhuman osmotic stress priority journal protein purification protein secretion rat refraction index regulatory mechanism scanning electron microscopy shear stress |
| spellingShingle |
Adhesion, aggregation ATP-transport E.coli hemolysin Hemolysis adenosine triphosphatase alpha hemolysin apyrase calcium carbenoxolone hemoglobin liposome purinergic P2X receptor animal experiment Article calcium cell level cell aggregation cell isolation cell shape cell structure cell swelling cell volume erythrocyte flow kinetics hemolysis hemolysis assay human human cell hydrolysis impedance male mathematical model membrane potential mesenteric arterial bed nonhuman osmotic stress priority journal protein purification protein secretion rat refraction index regulatory mechanism scanning electron microscopy shear stress Leal Denis, M.F. Lefevre, S.D. Alvarez, C.L. Lauri, N. Enrique, N. Rinaldi, D.E. Gonzalez-Lebrero, R. Vecchio, L.E. Espelt, M.V. Stringa, P. Muñoz-Garay, C. Milesi, V. Ostuni, M.A. Herlax, V. Schwarzbaum, P.J. Regulation of extracellular ATP of human erythrocytes treated with α-hemolysin. Effects of cell volume, morphology, rheology and hemolysis |
| topic_facet |
Adhesion, aggregation ATP-transport E.coli hemolysin Hemolysis adenosine triphosphatase alpha hemolysin apyrase calcium carbenoxolone hemoglobin liposome purinergic P2X receptor animal experiment Article calcium cell level cell aggregation cell isolation cell shape cell structure cell swelling cell volume erythrocyte flow kinetics hemolysis hemolysis assay human human cell hydrolysis impedance male mathematical model membrane potential mesenteric arterial bed nonhuman osmotic stress priority journal protein purification protein secretion rat refraction index regulatory mechanism scanning electron microscopy shear stress |
| description |
Alpha-hemolysin (HlyA) of uropathogenic strains of Escherichia coli irreversibly binds to human erythrocytes (RBCs) and triggers activation of ATP release and metabolic changes ultimately leading to hemolysis. We studied the regulation of extracellular ATP (ATPe) of RBCs exposed to HlyA. Luminometry was used to assess ATP release and ATPe hydrolysis, whereas changes in cell volume and morphology were determined by electrical impedance, ektacytometry and aggregometry. Exposure of RBCs to HlyA induced a strong increase of [ATPe] (3–36-fold) and hemolysis (1–44-fold), partially compensated by [ATPe] hydrolysis by ectoATPases and intracellular ATPases released by dead cells. Carbenoxolone, a pannexin 1 inhibitor, partially inhibited ATP release (43–67%). The un-acylated toxin ProHlyA and the deletion analog HlyA∆914-936 were unable to induce ATP release or hemolysis. For HlyA treated RBCs, a data driven mathematical model showed that simultaneous lytic and non-lytic release mainly governed ATPe kinetics, while ATPe hydrolysis became important after prolonged toxin exposure. HlyA induced a 1.5-fold swelling, while blocking this swelling reduced ATP release by 77%. Blocking ATPe activation of purinergic P2X receptors reduced swelling by 60–80%. HlyA-RBCs showed an acute 1.3–2.2-fold increase of Ca 2+ i, increased crenation and externalization of phosphatidylserine. Perfusion of HlyA-RBCs through adhesion platforms showed strong adhesion to activated HMEC cells, followed by rapid detachment. HlyA exposed RBCs exhibited increased sphericity under osmotic stress, reduced elongation under shear stress, and very low aggregation in viscous media. Overall results showed that HlyA-RBCs displayed activated ATP release, high but weak adhesivity, low deformability and aggregability and high sphericity. © 2019 Elsevier B.V. |
| format |
JOUR |
| author |
Leal Denis, M.F. Lefevre, S.D. Alvarez, C.L. Lauri, N. Enrique, N. Rinaldi, D.E. Gonzalez-Lebrero, R. Vecchio, L.E. Espelt, M.V. Stringa, P. Muñoz-Garay, C. Milesi, V. Ostuni, M.A. Herlax, V. Schwarzbaum, P.J. |
| author_facet |
Leal Denis, M.F. Lefevre, S.D. Alvarez, C.L. Lauri, N. Enrique, N. Rinaldi, D.E. Gonzalez-Lebrero, R. Vecchio, L.E. Espelt, M.V. Stringa, P. Muñoz-Garay, C. Milesi, V. Ostuni, M.A. Herlax, V. Schwarzbaum, P.J. |
| author_sort |
Leal Denis, M.F. |
| title |
Regulation of extracellular ATP of human erythrocytes treated with α-hemolysin. Effects of cell volume, morphology, rheology and hemolysis |
| title_short |
Regulation of extracellular ATP of human erythrocytes treated with α-hemolysin. Effects of cell volume, morphology, rheology and hemolysis |
| title_full |
Regulation of extracellular ATP of human erythrocytes treated with α-hemolysin. Effects of cell volume, morphology, rheology and hemolysis |
| title_fullStr |
Regulation of extracellular ATP of human erythrocytes treated with α-hemolysin. Effects of cell volume, morphology, rheology and hemolysis |
| title_full_unstemmed |
Regulation of extracellular ATP of human erythrocytes treated with α-hemolysin. Effects of cell volume, morphology, rheology and hemolysis |
| title_sort |
regulation of extracellular atp of human erythrocytes treated with α-hemolysin. effects of cell volume, morphology, rheology and hemolysis |
| url |
http://hdl.handle.net/20.500.12110/paper_01674889_v1866_n5_p896_LealDenis |
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