Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa
Phorbol esters stimulate cyclic adenosine 3′,5′-monophosphate (cAMP) accumulation in hamster spermatozoa under conditions for in vitro capacitation. The 20-50-fold elevation of cAMP levels induced by 1 μM phorbol 12-myristate 13-acetate (PMA) in spermatozoa depends on the presence of sodium bicarbon...
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todo:paper_01674889_v1054_n2_p231_Visconti2023-10-03T15:04:47Z Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa Visconti, P.E. Muschietti, J.P. Flawia, M.M. Tezon, J.G. (Hamster spermatozoa) Adenylate cyclase Bicarbonate cyclic AMP Phorbol myristate acetate adenylate cyclase bicarbonate cyclic amp phorbol ester animal cell article cell membrane hamster nonhuman priority journal spermatozoon 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid Adenylate Cyclase Animal Bicarbonates Biological Transport Cyclic AMP Enzyme Activation Epididymis Hamsters Hydrogen-Ion Concentration In Vitro Male Phorbol 12,13-Dibutyrate Sodium Sodium Bicarbonate Sperm Capacitation Spermatozoa Support, Non-U.S. Gov't Animalia Cricetinae Phorbol esters stimulate cyclic adenosine 3′,5′-monophosphate (cAMP) accumulation in hamster spermatozoa under conditions for in vitro capacitation. The 20-50-fold elevation of cAMP levels induced by 1 μM phorbol 12-myristate 13-acetate (PMA) in spermatozoa depends on the presence of sodium bicarbonate in the medium (ED50: 15 mM) and it is independent of extracellular pH. Sodium bicarbonate stimulates adenylate cyclase activity in membrane preparations by 4-fold (ED50: 40 mM). After sulubilization, the bicarbonate-sensitive moiety elutes as a single peak of 55 kDa in a gel filtration column. Blockers of bicarbonate chloride antiporters diisothiocyanate stilbene 2,2′-disulfonic acid (DIDS) or acetamido 4′-isothiocyanate stilbene 2,2′-disulfonic acid (SITS) inhibit the bicarbonate dependent PMA effect on cAMP in living spermatozoa (ED50: 100 μM). Maximal (85%) inhibition in cAMP accumulation is observed at 1 mM. Motility is inhibited only at high concentrations of the blockers. Pretreatment of living cells with 1 mM DIDS does not affect membrane adenylate cyclase activity which remains responsive to bicarbonate. These results suggest that controlled transport of bicarbonate through the sperm plasma membrane could be associated to the regulation of cAMP synthesis. © 1990. Fil:Muschietti, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawia, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tezon, J.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01674889_v1054_n2_p231_Visconti |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
(Hamster spermatozoa) Adenylate cyclase Bicarbonate cyclic AMP Phorbol myristate acetate adenylate cyclase bicarbonate cyclic amp phorbol ester animal cell article cell membrane hamster nonhuman priority journal spermatozoon 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid Adenylate Cyclase Animal Bicarbonates Biological Transport Cyclic AMP Enzyme Activation Epididymis Hamsters Hydrogen-Ion Concentration In Vitro Male Phorbol 12,13-Dibutyrate Sodium Sodium Bicarbonate Sperm Capacitation Spermatozoa Support, Non-U.S. Gov't Animalia Cricetinae |
| spellingShingle |
(Hamster spermatozoa) Adenylate cyclase Bicarbonate cyclic AMP Phorbol myristate acetate adenylate cyclase bicarbonate cyclic amp phorbol ester animal cell article cell membrane hamster nonhuman priority journal spermatozoon 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid Adenylate Cyclase Animal Bicarbonates Biological Transport Cyclic AMP Enzyme Activation Epididymis Hamsters Hydrogen-Ion Concentration In Vitro Male Phorbol 12,13-Dibutyrate Sodium Sodium Bicarbonate Sperm Capacitation Spermatozoa Support, Non-U.S. Gov't Animalia Cricetinae Visconti, P.E. Muschietti, J.P. Flawia, M.M. Tezon, J.G. Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa |
| topic_facet |
(Hamster spermatozoa) Adenylate cyclase Bicarbonate cyclic AMP Phorbol myristate acetate adenylate cyclase bicarbonate cyclic amp phorbol ester animal cell article cell membrane hamster nonhuman priority journal spermatozoon 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid Adenylate Cyclase Animal Bicarbonates Biological Transport Cyclic AMP Enzyme Activation Epididymis Hamsters Hydrogen-Ion Concentration In Vitro Male Phorbol 12,13-Dibutyrate Sodium Sodium Bicarbonate Sperm Capacitation Spermatozoa Support, Non-U.S. Gov't Animalia Cricetinae |
| description |
Phorbol esters stimulate cyclic adenosine 3′,5′-monophosphate (cAMP) accumulation in hamster spermatozoa under conditions for in vitro capacitation. The 20-50-fold elevation of cAMP levels induced by 1 μM phorbol 12-myristate 13-acetate (PMA) in spermatozoa depends on the presence of sodium bicarbonate in the medium (ED50: 15 mM) and it is independent of extracellular pH. Sodium bicarbonate stimulates adenylate cyclase activity in membrane preparations by 4-fold (ED50: 40 mM). After sulubilization, the bicarbonate-sensitive moiety elutes as a single peak of 55 kDa in a gel filtration column. Blockers of bicarbonate chloride antiporters diisothiocyanate stilbene 2,2′-disulfonic acid (DIDS) or acetamido 4′-isothiocyanate stilbene 2,2′-disulfonic acid (SITS) inhibit the bicarbonate dependent PMA effect on cAMP in living spermatozoa (ED50: 100 μM). Maximal (85%) inhibition in cAMP accumulation is observed at 1 mM. Motility is inhibited only at high concentrations of the blockers. Pretreatment of living cells with 1 mM DIDS does not affect membrane adenylate cyclase activity which remains responsive to bicarbonate. These results suggest that controlled transport of bicarbonate through the sperm plasma membrane could be associated to the regulation of cAMP synthesis. © 1990. |
| format |
JOUR |
| author |
Visconti, P.E. Muschietti, J.P. Flawia, M.M. Tezon, J.G. |
| author_facet |
Visconti, P.E. Muschietti, J.P. Flawia, M.M. Tezon, J.G. |
| author_sort |
Visconti, P.E. |
| title |
Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa |
| title_short |
Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa |
| title_full |
Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa |
| title_fullStr |
Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa |
| title_full_unstemmed |
Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa |
| title_sort |
bicarbonate dependence of camp accumulation induced by phorbol esters in hamster spermatozoa |
| url |
http://hdl.handle.net/20.500.12110/paper_01674889_v1054_n2_p231_Visconti |
| work_keys_str_mv |
AT viscontipe bicarbonatedependenceofcampaccumulationinducedbyphorbolestersinhamsterspermatozoa AT muschiettijp bicarbonatedependenceofcampaccumulationinducedbyphorbolestersinhamsterspermatozoa AT flawiamm bicarbonatedependenceofcampaccumulationinducedbyphorbolestersinhamsterspermatozoa AT tezonjg bicarbonatedependenceofcampaccumulationinducedbyphorbolestersinhamsterspermatozoa |
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1782031029337325568 |