A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi

A full-length DNA clone encoding a putative pyruvate dehydrogenase α subunit (E1α) gene was isolated from a Trypanosoma cruzi (RA strain) DNA library. Sequencing of this clone revealed it to encode a 378 amino acid protein (M(r) 42774) with high sequence similarity to E1α obtained from different sou...

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Autores principales: Buscaglia, C.A., Pollevick, G.D., Veloso, C., Lorca, M., Frasch, A.C.C., Sánchez, D.O.
Formato: JOUR
Materias:
(Trypanosoma cruzi)
Pyruvate dehydrogenase α subunit
Trans-splicing
pyruvate dehydrogenase
amino acid sequence
article
dna library
enzyme subunit
gene isolation
human
molecular cloning
nonhuman
nucleotide sequence
priority journal
sequence homology
trypanosoma cruzi
yeast
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA, Protozoan
Gene Dosage
Genes, Protozoan
Humans
Molecular Sequence Data
Pyruvate Dehydrogenase Complex
RNA, Messenger
RNA, Protozoan
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Trypanosoma cruzi
Trypanosoma
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01674781_v1309_n1-2_p53_Buscaglia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01674781_v1309_n1-2_p53_Buscaglia
record_format dspace
spelling todo:paper_01674781_v1309_n1-2_p53_Buscaglia2023-10-03T15:04:45Z A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi Buscaglia, C.A. Pollevick, G.D. Veloso, C. Lorca, M. Frasch, A.C.C. Sánchez, D.O. (Trypanosoma cruzi) Pyruvate dehydrogenase α subunit Trans-splicing pyruvate dehydrogenase amino acid sequence article dna library enzyme subunit gene isolation human molecular cloning nonhuman nucleotide sequence priority journal sequence homology trypanosoma cruzi yeast Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Protozoan Gene Dosage Genes, Protozoan Humans Molecular Sequence Data Pyruvate Dehydrogenase Complex RNA, Messenger RNA, Protozoan Sequence Analysis, DNA Sequence Homology, Amino Acid Trypanosoma cruzi Trypanosoma Trypanosoma cruzi A full-length DNA clone encoding a putative pyruvate dehydrogenase α subunit (E1α) gene was isolated from a Trypanosoma cruzi (RA strain) DNA library. Sequencing of this clone revealed it to encode a 378 amino acid protein (M(r) 42774) with high sequence similarity to E1α obtained from different sources. The highest score is obtained with human E1α: 43.3% similarity. Southern blot analysis is consistent with the existence of a single copy of this putative T. cruzi E1α gene per haploid genome in different parasite strains. Expression of this gene was demonstrated by Northern blot analysis and its trans-splicing acceptor site was identified by Polymerase Chain Reaction-mediated amplification of its cDNA. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01674781_v1309_n1-2_p53_Buscaglia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic (Trypanosoma cruzi)
Pyruvate dehydrogenase α subunit
Trans-splicing
pyruvate dehydrogenase
amino acid sequence
article
dna library
enzyme subunit
gene isolation
human
molecular cloning
nonhuman
nucleotide sequence
priority journal
sequence homology
trypanosoma cruzi
yeast
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA, Protozoan
Gene Dosage
Genes, Protozoan
Humans
Molecular Sequence Data
Pyruvate Dehydrogenase Complex
RNA, Messenger
RNA, Protozoan
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Trypanosoma cruzi
Trypanosoma
Trypanosoma cruzi
spellingShingle (Trypanosoma cruzi)
Pyruvate dehydrogenase α subunit
Trans-splicing
pyruvate dehydrogenase
amino acid sequence
article
dna library
enzyme subunit
gene isolation
human
molecular cloning
nonhuman
nucleotide sequence
priority journal
sequence homology
trypanosoma cruzi
yeast
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA, Protozoan
Gene Dosage
Genes, Protozoan
Humans
Molecular Sequence Data
Pyruvate Dehydrogenase Complex
RNA, Messenger
RNA, Protozoan
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Trypanosoma cruzi
Trypanosoma
Trypanosoma cruzi
Buscaglia, C.A.
Pollevick, G.D.
Veloso, C.
Lorca, M.
Frasch, A.C.C.
Sánchez, D.O.
A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi
topic_facet (Trypanosoma cruzi)
Pyruvate dehydrogenase α subunit
Trans-splicing
pyruvate dehydrogenase
amino acid sequence
article
dna library
enzyme subunit
gene isolation
human
molecular cloning
nonhuman
nucleotide sequence
priority journal
sequence homology
trypanosoma cruzi
yeast
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA, Protozoan
Gene Dosage
Genes, Protozoan
Humans
Molecular Sequence Data
Pyruvate Dehydrogenase Complex
RNA, Messenger
RNA, Protozoan
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Trypanosoma cruzi
Trypanosoma
Trypanosoma cruzi
description A full-length DNA clone encoding a putative pyruvate dehydrogenase α subunit (E1α) gene was isolated from a Trypanosoma cruzi (RA strain) DNA library. Sequencing of this clone revealed it to encode a 378 amino acid protein (M(r) 42774) with high sequence similarity to E1α obtained from different sources. The highest score is obtained with human E1α: 43.3% similarity. Southern blot analysis is consistent with the existence of a single copy of this putative T. cruzi E1α gene per haploid genome in different parasite strains. Expression of this gene was demonstrated by Northern blot analysis and its trans-splicing acceptor site was identified by Polymerase Chain Reaction-mediated amplification of its cDNA.
format JOUR
author Buscaglia, C.A.
Pollevick, G.D.
Veloso, C.
Lorca, M.
Frasch, A.C.C.
Sánchez, D.O.
author_facet Buscaglia, C.A.
Pollevick, G.D.
Veloso, C.
Lorca, M.
Frasch, A.C.C.
Sánchez, D.O.
author_sort Buscaglia, C.A.
title A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi
title_short A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi
title_full A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi
title_fullStr A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi
title_full_unstemmed A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi
title_sort putative pyruvate dehydrogenase α subunit gene from trypanosoma cruzi
url http://hdl.handle.net/20.500.12110/paper_01674781_v1309_n1-2_p53_Buscaglia
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