Polyamines modulate streptomycin-induced mistranslation in Escherichia coli
The effects of intracellular levels of polyamines on both the in vivo inhibition of protein synthesis and the decrease of translation accuracy induced by streptomycin have been studied in polyamine-auxotrophic strains of Escherichia coli infected with the MS2 bacteriophage. The amount of viral coat...
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todo:paper_01674781_v1216_n3_p455_Nastri2023-10-03T15:04:44Z Polyamines modulate streptomycin-induced mistranslation in Escherichia coli Nastri, H.G. Fastame, I.G. Algranati, I.D. (E. coli) Polyamine Streptomycin Translation fidelity coat protein polyamine streptomycin article bacteriophage controlled study escherichia coli nonhuman priority journal rna translation virus infection Autoradiography Bacterial Proteins Capsid Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Escherichia coli Levivirus Methionine Molecular Weight Polyamines Streptomycin Support, Non-U.S. Gov't Translation, Genetic Escherichia coli The effects of intracellular levels of polyamines on both the in vivo inhibition of protein synthesis and the decrease of translation accuracy induced by streptomycin have been studied in polyamine-auxotrophic strains of Escherichia coli infected with the MS2 bacteriophage. The amount of viral coat protein formed was strongly reduced upon addition of increasing concentrations of streptomycin to polyamine-supplemented bacteria. In contrast, the antibiotic almost did not inhibit coat protein synthesis in polyamine-starved cells. The increase of mistranslation frequency elicited by streptomycin was only observed in bacteria grown with putrescine. In these cells several coat protein-satellites were detected after two-dimensional gel electrophoresis. These proteins, more basic than the normal MS2 coat protein, contain multiple substitutions of lysine for asparagine. © 1993. Fil:Nastri, H.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fastame, I.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01674781_v1216_n3_p455_Nastri |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
(E. coli) Polyamine Streptomycin Translation fidelity coat protein polyamine streptomycin article bacteriophage controlled study escherichia coli nonhuman priority journal rna translation virus infection Autoradiography Bacterial Proteins Capsid Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Escherichia coli Levivirus Methionine Molecular Weight Polyamines Streptomycin Support, Non-U.S. Gov't Translation, Genetic Escherichia coli |
| spellingShingle |
(E. coli) Polyamine Streptomycin Translation fidelity coat protein polyamine streptomycin article bacteriophage controlled study escherichia coli nonhuman priority journal rna translation virus infection Autoradiography Bacterial Proteins Capsid Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Escherichia coli Levivirus Methionine Molecular Weight Polyamines Streptomycin Support, Non-U.S. Gov't Translation, Genetic Escherichia coli Nastri, H.G. Fastame, I.G. Algranati, I.D. Polyamines modulate streptomycin-induced mistranslation in Escherichia coli |
| topic_facet |
(E. coli) Polyamine Streptomycin Translation fidelity coat protein polyamine streptomycin article bacteriophage controlled study escherichia coli nonhuman priority journal rna translation virus infection Autoradiography Bacterial Proteins Capsid Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Escherichia coli Levivirus Methionine Molecular Weight Polyamines Streptomycin Support, Non-U.S. Gov't Translation, Genetic Escherichia coli |
| description |
The effects of intracellular levels of polyamines on both the in vivo inhibition of protein synthesis and the decrease of translation accuracy induced by streptomycin have been studied in polyamine-auxotrophic strains of Escherichia coli infected with the MS2 bacteriophage. The amount of viral coat protein formed was strongly reduced upon addition of increasing concentrations of streptomycin to polyamine-supplemented bacteria. In contrast, the antibiotic almost did not inhibit coat protein synthesis in polyamine-starved cells. The increase of mistranslation frequency elicited by streptomycin was only observed in bacteria grown with putrescine. In these cells several coat protein-satellites were detected after two-dimensional gel electrophoresis. These proteins, more basic than the normal MS2 coat protein, contain multiple substitutions of lysine for asparagine. © 1993. |
| format |
JOUR |
| author |
Nastri, H.G. Fastame, I.G. Algranati, I.D. |
| author_facet |
Nastri, H.G. Fastame, I.G. Algranati, I.D. |
| author_sort |
Nastri, H.G. |
| title |
Polyamines modulate streptomycin-induced mistranslation in Escherichia coli |
| title_short |
Polyamines modulate streptomycin-induced mistranslation in Escherichia coli |
| title_full |
Polyamines modulate streptomycin-induced mistranslation in Escherichia coli |
| title_fullStr |
Polyamines modulate streptomycin-induced mistranslation in Escherichia coli |
| title_full_unstemmed |
Polyamines modulate streptomycin-induced mistranslation in Escherichia coli |
| title_sort |
polyamines modulate streptomycin-induced mistranslation in escherichia coli |
| url |
http://hdl.handle.net/20.500.12110/paper_01674781_v1216_n3_p455_Nastri |
| work_keys_str_mv |
AT nastrihg polyaminesmodulatestreptomycininducedmistranslationinescherichiacoli AT fastameig polyaminesmodulatestreptomycininducedmistranslationinescherichiacoli AT algranatiid polyaminesmodulatestreptomycininducedmistranslationinescherichiacoli |
| _version_ |
1807317831045349376 |