Genomic and functional characterization of StCDPK1
StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene p...
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todo:paper_01674412_v70_n1-2_p153_Gargantini2023-10-03T15:04:43Z Genomic and functional characterization of StCDPK1 Gargantini, P.R. Giammaria, V. Grandellis, C. Feingold, S.E. Maldonado, S. Ulloa, R.M. Calcium-dependent protein kinases Gibberellins Solanum tuberosum Tuberization calcium binding protein CDPK1 protein, plant gibberellin phytohormone plant DNA protein kinase vegetable protein article chromosome map genetics metabolism molecular cloning plant tuber potato transgenic plant Calcium-Binding Proteins Chromosome Mapping Cloning, Molecular DNA, Plant Gibberellins Plant Growth Regulators Plant Proteins Plant Tubers Plants, Genetically Modified Protein Kinases Solanum tuberosum Arabidopsis Solanum tuberosum Triticum aestivum Tuber (truffle) StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene per genome and it is located in the distal portion of chromosome 12. Western blot and immunolocalization assays (using confocal and transmission electron microscopy) performed with a specific antibody against StCDPK1 indicate that this kinase is mainly located in the plasma membrane of swelling stolons and sprouting tubers. Sucrose (4-8%) increased StCDPK1 protein content in non-induced stolons, however the amount detected in swelling stolons was higher. Transgenic lines with reduced expression of StCDPK1 (β7) did not differ from controls when cultured under multiplication conditions, but when grown under tuber inducing conditions some significant differences were observed: the β7 line tuberized earlier than controls without the addition of CCC (GA inhibitor), developed more tubers than wild type plants in the presence of hormones that promote tuberization in potato (ABA and BAP) and was more insensitive to GA action (stolons were significantly shorter than those of control plants). StCDPK1 expression was induced by GA, ABA and BAP. Our results suggest that StCDPK1 plays a role in GA-signalling and that this kinase could be a converging point for the inhibitory and promoting signals that influence the onset of potato tuberization. © 2009 Springer Science+Business Media B.V. Fil:Gargantini, P.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giammaria, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grandellis, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Feingold, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01674412_v70_n1-2_p153_Gargantini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Calcium-dependent protein kinases Gibberellins Solanum tuberosum Tuberization calcium binding protein CDPK1 protein, plant gibberellin phytohormone plant DNA protein kinase vegetable protein article chromosome map genetics metabolism molecular cloning plant tuber potato transgenic plant Calcium-Binding Proteins Chromosome Mapping Cloning, Molecular DNA, Plant Gibberellins Plant Growth Regulators Plant Proteins Plant Tubers Plants, Genetically Modified Protein Kinases Solanum tuberosum Arabidopsis Solanum tuberosum Triticum aestivum Tuber (truffle) |
| spellingShingle |
Calcium-dependent protein kinases Gibberellins Solanum tuberosum Tuberization calcium binding protein CDPK1 protein, plant gibberellin phytohormone plant DNA protein kinase vegetable protein article chromosome map genetics metabolism molecular cloning plant tuber potato transgenic plant Calcium-Binding Proteins Chromosome Mapping Cloning, Molecular DNA, Plant Gibberellins Plant Growth Regulators Plant Proteins Plant Tubers Plants, Genetically Modified Protein Kinases Solanum tuberosum Arabidopsis Solanum tuberosum Triticum aestivum Tuber (truffle) Gargantini, P.R. Giammaria, V. Grandellis, C. Feingold, S.E. Maldonado, S. Ulloa, R.M. Genomic and functional characterization of StCDPK1 |
| topic_facet |
Calcium-dependent protein kinases Gibberellins Solanum tuberosum Tuberization calcium binding protein CDPK1 protein, plant gibberellin phytohormone plant DNA protein kinase vegetable protein article chromosome map genetics metabolism molecular cloning plant tuber potato transgenic plant Calcium-Binding Proteins Chromosome Mapping Cloning, Molecular DNA, Plant Gibberellins Plant Growth Regulators Plant Proteins Plant Tubers Plants, Genetically Modified Protein Kinases Solanum tuberosum Arabidopsis Solanum tuberosum Triticum aestivum Tuber (truffle) |
| description |
StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene per genome and it is located in the distal portion of chromosome 12. Western blot and immunolocalization assays (using confocal and transmission electron microscopy) performed with a specific antibody against StCDPK1 indicate that this kinase is mainly located in the plasma membrane of swelling stolons and sprouting tubers. Sucrose (4-8%) increased StCDPK1 protein content in non-induced stolons, however the amount detected in swelling stolons was higher. Transgenic lines with reduced expression of StCDPK1 (β7) did not differ from controls when cultured under multiplication conditions, but when grown under tuber inducing conditions some significant differences were observed: the β7 line tuberized earlier than controls without the addition of CCC (GA inhibitor), developed more tubers than wild type plants in the presence of hormones that promote tuberization in potato (ABA and BAP) and was more insensitive to GA action (stolons were significantly shorter than those of control plants). StCDPK1 expression was induced by GA, ABA and BAP. Our results suggest that StCDPK1 plays a role in GA-signalling and that this kinase could be a converging point for the inhibitory and promoting signals that influence the onset of potato tuberization. © 2009 Springer Science+Business Media B.V. |
| format |
JOUR |
| author |
Gargantini, P.R. Giammaria, V. Grandellis, C. Feingold, S.E. Maldonado, S. Ulloa, R.M. |
| author_facet |
Gargantini, P.R. Giammaria, V. Grandellis, C. Feingold, S.E. Maldonado, S. Ulloa, R.M. |
| author_sort |
Gargantini, P.R. |
| title |
Genomic and functional characterization of StCDPK1 |
| title_short |
Genomic and functional characterization of StCDPK1 |
| title_full |
Genomic and functional characterization of StCDPK1 |
| title_fullStr |
Genomic and functional characterization of StCDPK1 |
| title_full_unstemmed |
Genomic and functional characterization of StCDPK1 |
| title_sort |
genomic and functional characterization of stcdpk1 |
| url |
http://hdl.handle.net/20.500.12110/paper_01674412_v70_n1-2_p153_Gargantini |
| work_keys_str_mv |
AT gargantinipr genomicandfunctionalcharacterizationofstcdpk1 AT giammariav genomicandfunctionalcharacterizationofstcdpk1 AT grandellisc genomicandfunctionalcharacterizationofstcdpk1 AT feingoldse genomicandfunctionalcharacterizationofstcdpk1 AT maldonados genomicandfunctionalcharacterizationofstcdpk1 AT ulloarm genomicandfunctionalcharacterizationofstcdpk1 |
| _version_ |
1807322521392906240 |