ci21A/Asr1 expression influences glucose accumulation in potato tubers

Asr genes are exclusively found in the genomes of higher plants. In many species, this gene family is expressed under abiotic stress conditions and during fruit ripening. The encoded proteins have nuclear localisation and consequently a transcription factor function has been suggested. Interestingly...

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Autores principales:	Frankel, N., Nunes-Nesi, A., Balbo, I., Mazuch, J., Centeno, D., Iusem, N.D., Fernie, A.R., Carrari, F.
Formato:	JOUR
Materias:	Asr genes Hexose metabolism Potato tuber Tomato Binding energy Biodiversity Glucose Plant cell culture Plants (botany) Genes Ci21A protein, Solanum tuberosum glucose heat shock protein messenger RNA primer DNA unclassified drug vegetable protein article gene expression regulation genetics metabolism photosynthesis plant plant root potato reverse transcription polymerase chain reaction transgenic plant DNA Primers Gene Expression Regulation, Plant Heat-Shock Proteins Photosynthesis Plant Components, Aerial Plant Proteins Plant Roots Plants, Genetically Modified Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Solanum tuberosum Binding Cells Phenotypes Plants Tomatoes Embryophyta Lycopersicon esculentum Tuber (truffle) Vitaceae
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01674412_v63_n5_p719_Frankel
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01674412_v63_n5_p719_Frankel
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spelling	todo:paper_01674412_v63_n5_p719_Frankel2023-10-03T15:04:42Z ci21A/Asr1 expression influences glucose accumulation in potato tubers Frankel, N. Nunes-Nesi, A. Balbo, I. Mazuch, J. Centeno, D. Iusem, N.D. Fernie, A.R. Carrari, F. Asr genes Hexose metabolism Potato tuber Tomato Asr genes Hexose metabolism Potato tuber Binding energy Biodiversity Glucose Plant cell culture Plants (botany) Genes Ci21A protein, Solanum tuberosum glucose heat shock protein messenger RNA primer DNA unclassified drug vegetable protein article gene expression regulation genetics metabolism photosynthesis plant plant root potato reverse transcription polymerase chain reaction transgenic plant DNA Primers Gene Expression Regulation, Plant Glucose Heat-Shock Proteins Photosynthesis Plant Components, Aerial Plant Proteins Plant Roots Plants, Genetically Modified Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Solanum tuberosum Binding Cells Genes Glucose Phenotypes Plants Tomatoes Embryophyta Lycopersicon esculentum Solanum tuberosum Tuber (truffle) Vitaceae Asr genes are exclusively found in the genomes of higher plants. In many species, this gene family is expressed under abiotic stress conditions and during fruit ripening. The encoded proteins have nuclear localisation and consequently a transcription factor function has been suggested. Interestingly, yeast-one-hybrid experiments revealed that a grape ASR binds to the promoter of a hexose transporter gene (VvHT1). However, the role of these proteins in planta is still elusive. By using a reverse genetics approach in potato we found that modification of Asr1 expression has no incidence on the aerial phenotype of the plant but exerts a dramatic effect in tuber. Asr1 antisense potatoes displayed decreased tuber fresh weight whereas Asr1 overexpressors had a diminished number of tubers. Moreover, overexpression lines showed lower transcript levels of a plasma membrane hexose transporter and a concomitant decrease in glucose content in parenchyma cells of potato tubers. On the same hand glucose uptake rate was also reduced in one of the overexpressing lines. It thus seems likely that Asr1 is involved in the control of hexose uptake in heterotrophic organs. In addition, the transgenic plants were characterized by several other changes in steady state metabolite levels. Results presented here support a role for ci21A/Asr1 in glucose metabolism of potato tuber. © 2007 Springer Science+Business Media B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01674412_v63_n5_p719_Frankel
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Asr genes Hexose metabolism Potato tuber Tomato Asr genes Hexose metabolism Potato tuber Binding energy Biodiversity Glucose Plant cell culture Plants (botany) Genes Ci21A protein, Solanum tuberosum glucose heat shock protein messenger RNA primer DNA unclassified drug vegetable protein article gene expression regulation genetics metabolism photosynthesis plant plant root potato reverse transcription polymerase chain reaction transgenic plant DNA Primers Gene Expression Regulation, Plant Glucose Heat-Shock Proteins Photosynthesis Plant Components, Aerial Plant Proteins Plant Roots Plants, Genetically Modified Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Solanum tuberosum Binding Cells Genes Glucose Phenotypes Plants Tomatoes Embryophyta Lycopersicon esculentum Solanum tuberosum Tuber (truffle) Vitaceae
spellingShingle	Asr genes Hexose metabolism Potato tuber Tomato Asr genes Hexose metabolism Potato tuber Binding energy Biodiversity Glucose Plant cell culture Plants (botany) Genes Ci21A protein, Solanum tuberosum glucose heat shock protein messenger RNA primer DNA unclassified drug vegetable protein article gene expression regulation genetics metabolism photosynthesis plant plant root potato reverse transcription polymerase chain reaction transgenic plant DNA Primers Gene Expression Regulation, Plant Glucose Heat-Shock Proteins Photosynthesis Plant Components, Aerial Plant Proteins Plant Roots Plants, Genetically Modified Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Solanum tuberosum Binding Cells Genes Glucose Phenotypes Plants Tomatoes Embryophyta Lycopersicon esculentum Solanum tuberosum Tuber (truffle) Vitaceae Frankel, N. Nunes-Nesi, A. Balbo, I. Mazuch, J. Centeno, D. Iusem, N.D. Fernie, A.R. Carrari, F. ci21A/Asr1 expression influences glucose accumulation in potato tubers
topic_facet	Asr genes Hexose metabolism Potato tuber Tomato Asr genes Hexose metabolism Potato tuber Binding energy Biodiversity Glucose Plant cell culture Plants (botany) Genes Ci21A protein, Solanum tuberosum glucose heat shock protein messenger RNA primer DNA unclassified drug vegetable protein article gene expression regulation genetics metabolism photosynthesis plant plant root potato reverse transcription polymerase chain reaction transgenic plant DNA Primers Gene Expression Regulation, Plant Glucose Heat-Shock Proteins Photosynthesis Plant Components, Aerial Plant Proteins Plant Roots Plants, Genetically Modified Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Solanum tuberosum Binding Cells Genes Glucose Phenotypes Plants Tomatoes Embryophyta Lycopersicon esculentum Solanum tuberosum Tuber (truffle) Vitaceae
description	Asr genes are exclusively found in the genomes of higher plants. In many species, this gene family is expressed under abiotic stress conditions and during fruit ripening. The encoded proteins have nuclear localisation and consequently a transcription factor function has been suggested. Interestingly, yeast-one-hybrid experiments revealed that a grape ASR binds to the promoter of a hexose transporter gene (VvHT1). However, the role of these proteins in planta is still elusive. By using a reverse genetics approach in potato we found that modification of Asr1 expression has no incidence on the aerial phenotype of the plant but exerts a dramatic effect in tuber. Asr1 antisense potatoes displayed decreased tuber fresh weight whereas Asr1 overexpressors had a diminished number of tubers. Moreover, overexpression lines showed lower transcript levels of a plasma membrane hexose transporter and a concomitant decrease in glucose content in parenchyma cells of potato tubers. On the same hand glucose uptake rate was also reduced in one of the overexpressing lines. It thus seems likely that Asr1 is involved in the control of hexose uptake in heterotrophic organs. In addition, the transgenic plants were characterized by several other changes in steady state metabolite levels. Results presented here support a role for ci21A/Asr1 in glucose metabolism of potato tuber. © 2007 Springer Science+Business Media B.V.
format	JOUR
author	Frankel, N. Nunes-Nesi, A. Balbo, I. Mazuch, J. Centeno, D. Iusem, N.D. Fernie, A.R. Carrari, F.
author_facet	Frankel, N. Nunes-Nesi, A. Balbo, I. Mazuch, J. Centeno, D. Iusem, N.D. Fernie, A.R. Carrari, F.
author_sort	Frankel, N.
title	ci21A/Asr1 expression influences glucose accumulation in potato tubers
title_short	ci21A/Asr1 expression influences glucose accumulation in potato tubers
title_full	ci21A/Asr1 expression influences glucose accumulation in potato tubers
title_fullStr	ci21A/Asr1 expression influences glucose accumulation in potato tubers
title_full_unstemmed	ci21A/Asr1 expression influences glucose accumulation in potato tubers
title_sort	ci21a/asr1 expression influences glucose accumulation in potato tubers
url	http://hdl.handle.net/20.500.12110/paper_01674412_v63_n5_p719_Frankel
work_keys_str_mv	AT frankeln ci21aasr1expressioninfluencesglucoseaccumulationinpotatotubers AT nunesnesia ci21aasr1expressioninfluencesglucoseaccumulationinpotatotubers AT balboi ci21aasr1expressioninfluencesglucoseaccumulationinpotatotubers AT mazuchj ci21aasr1expressioninfluencesglucoseaccumulationinpotatotubers AT centenod ci21aasr1expressioninfluencesglucoseaccumulationinpotatotubers AT iusemnd ci21aasr1expressioninfluencesglucoseaccumulationinpotatotubers AT ferniear ci21aasr1expressioninfluencesglucoseaccumulationinpotatotubers AT carrarif ci21aasr1expressioninfluencesglucoseaccumulationinpotatotubers
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ci21A/Asr1 expression influences glucose accumulation in potato tubers

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