Temperature acclimation of Trypanosoma cruzi epimastigote and metacyclic trypomastigote lipids
This study examines the changes in cellular lipids that take place when Trypanosoma cruzi epimastigotes and metacyclic trypomastigotes are transferred from 28 to 37°C. We found a rise in the sterol to phospholipid ratio, as well as in the triacylglycerol and steryl ester cellular content in T. cruzi...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01666851_v88_n1-2_p25_FlorinChristensen |
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todo:paper_01666851_v88_n1-2_p25_FlorinChristensen2023-10-03T15:04:25Z Temperature acclimation of Trypanosoma cruzi epimastigote and metacyclic trypomastigote lipids Florin-Christensen, M. Florin-Christensen, J. De Isola, E.D. Lammel, E. Meinardi, E. Brenner, R.R. Rasmussen, L. Fatty acids Lipids Phospholipids Sterols Temperature Trypanosoma cruzi fatty acid lipid sterol article epimastigote fatty acid desaturation life cycle lipid composition lipid metabolism priority journal temperature acclimatization trypanosoma cruzi trypomastigote Adaptation, Physiological Animals Fatty Acid Desaturases Fatty Acids Lipid Metabolism Lipids Phospholipids Sterols Temperature Triglycerides Trypanosoma cruzi Trypanosoma Trypanosoma cruzi This study examines the changes in cellular lipids that take place when Trypanosoma cruzi epimastigotes and metacyclic trypomastigotes are transferred from 28 to 37°C. We found a rise in the sterol to phospholipid ratio, as well as in the triacylglycerol and steryl ester cellular content in T. cruzi epimastigotes. In addition, saturated to unsaturated fatty acid ratios in phospholipids increase. This latter effect appears to be due to two concurrent processes. Firstly, fatty acyl Δ9 and, especially, Δ12 desaturations are significantly diminished at 37°C. Secondly, triacylglycerols and steryl esters undergo changes in their fatty acyl composition opposite to those simultaneously observed in phospholipids, i.e. the ratio of saturated to unsaturated fatty acids markedly decreases. Similar alterations in each of the lipid classes and in the fatty acid composition of polar and neutral lipids were found in cultured metacyclic trypomastigotes on exposure to the same shift-up. These observations suggest that a global remodeling of cellular lipids that involves extensive fatty acid exchange between neutral and polar lipid pools represents a novel and important mechanism of adaptation of the parasites to the temperature changes they encounter in their life cycle. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01666851_v88_n1-2_p25_FlorinChristensen |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Fatty acids Lipids Phospholipids Sterols Temperature Trypanosoma cruzi fatty acid lipid sterol article epimastigote fatty acid desaturation life cycle lipid composition lipid metabolism priority journal temperature acclimatization trypanosoma cruzi trypomastigote Adaptation, Physiological Animals Fatty Acid Desaturases Fatty Acids Lipid Metabolism Lipids Phospholipids Sterols Temperature Triglycerides Trypanosoma cruzi Trypanosoma Trypanosoma cruzi |
| spellingShingle |
Fatty acids Lipids Phospholipids Sterols Temperature Trypanosoma cruzi fatty acid lipid sterol article epimastigote fatty acid desaturation life cycle lipid composition lipid metabolism priority journal temperature acclimatization trypanosoma cruzi trypomastigote Adaptation, Physiological Animals Fatty Acid Desaturases Fatty Acids Lipid Metabolism Lipids Phospholipids Sterols Temperature Triglycerides Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Florin-Christensen, M. Florin-Christensen, J. De Isola, E.D. Lammel, E. Meinardi, E. Brenner, R.R. Rasmussen, L. Temperature acclimation of Trypanosoma cruzi epimastigote and metacyclic trypomastigote lipids |
| topic_facet |
Fatty acids Lipids Phospholipids Sterols Temperature Trypanosoma cruzi fatty acid lipid sterol article epimastigote fatty acid desaturation life cycle lipid composition lipid metabolism priority journal temperature acclimatization trypanosoma cruzi trypomastigote Adaptation, Physiological Animals Fatty Acid Desaturases Fatty Acids Lipid Metabolism Lipids Phospholipids Sterols Temperature Triglycerides Trypanosoma cruzi Trypanosoma Trypanosoma cruzi |
| description |
This study examines the changes in cellular lipids that take place when Trypanosoma cruzi epimastigotes and metacyclic trypomastigotes are transferred from 28 to 37°C. We found a rise in the sterol to phospholipid ratio, as well as in the triacylglycerol and steryl ester cellular content in T. cruzi epimastigotes. In addition, saturated to unsaturated fatty acid ratios in phospholipids increase. This latter effect appears to be due to two concurrent processes. Firstly, fatty acyl Δ9 and, especially, Δ12 desaturations are significantly diminished at 37°C. Secondly, triacylglycerols and steryl esters undergo changes in their fatty acyl composition opposite to those simultaneously observed in phospholipids, i.e. the ratio of saturated to unsaturated fatty acids markedly decreases. Similar alterations in each of the lipid classes and in the fatty acid composition of polar and neutral lipids were found in cultured metacyclic trypomastigotes on exposure to the same shift-up. These observations suggest that a global remodeling of cellular lipids that involves extensive fatty acid exchange between neutral and polar lipid pools represents a novel and important mechanism of adaptation of the parasites to the temperature changes they encounter in their life cycle. |
| format |
JOUR |
| author |
Florin-Christensen, M. Florin-Christensen, J. De Isola, E.D. Lammel, E. Meinardi, E. Brenner, R.R. Rasmussen, L. |
| author_facet |
Florin-Christensen, M. Florin-Christensen, J. De Isola, E.D. Lammel, E. Meinardi, E. Brenner, R.R. Rasmussen, L. |
| author_sort |
Florin-Christensen, M. |
| title |
Temperature acclimation of Trypanosoma cruzi epimastigote and metacyclic trypomastigote lipids |
| title_short |
Temperature acclimation of Trypanosoma cruzi epimastigote and metacyclic trypomastigote lipids |
| title_full |
Temperature acclimation of Trypanosoma cruzi epimastigote and metacyclic trypomastigote lipids |
| title_fullStr |
Temperature acclimation of Trypanosoma cruzi epimastigote and metacyclic trypomastigote lipids |
| title_full_unstemmed |
Temperature acclimation of Trypanosoma cruzi epimastigote and metacyclic trypomastigote lipids |
| title_sort |
temperature acclimation of trypanosoma cruzi epimastigote and metacyclic trypomastigote lipids |
| url |
http://hdl.handle.net/20.500.12110/paper_01666851_v88_n1-2_p25_FlorinChristensen |
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