Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi

The carbohydrate moiety of the Tc-85 surface glycoprotein from the infective trypomastigote form of Trypanosoma cruzi was analysed. Tc-85 could be metabolically labeled by incubation of the cells with d-[14C]mannose or d-[14C]glucose. Degradation techniques were performed directly on the polyacrylam...

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Autores principales: Couto, A.S., Katzin, A.M., Colli, W., de Lederkremer, R.M.
Formato: JOUR
Materias:
Glycoprotein carbohydrate composition
Sialic acid
Trypanosoma cruzi
Trypomastigote
glycoprotein
membrane protein
n acetylneuraminic acid
oligosaccharide
sialic acid derivative
affinity chromatography
animal
article
gel chromatography
paper chromatography
paper electrophoresis
Animal
Chromatography, Affinity
Chromatography, Gel
Chromatography, Paper
Electrophoresis, Paper
Glycoproteins
Membrane Proteins
N-Acetylneuraminic Acid
Oligosaccharides
Sialic Acids
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01666851_v26_n1-2_p145_Couto
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01666851_v26_n1-2_p145_Couto
record_format dspace
spelling todo:paper_01666851_v26_n1-2_p145_Couto2023-10-03T15:04:18Z Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi Couto, A.S. Katzin, A.M. Colli, W. de Lederkremer, R.M. Glycoprotein carbohydrate composition Sialic acid Trypanosoma cruzi Trypomastigote glycoprotein membrane protein n acetylneuraminic acid oligosaccharide sialic acid derivative affinity chromatography animal article gel chromatography paper chromatography paper electrophoresis Trypanosoma cruzi Animal Chromatography, Affinity Chromatography, Gel Chromatography, Paper Electrophoresis, Paper Glycoproteins Membrane Proteins N-Acetylneuraminic Acid Oligosaccharides Sialic Acids Support, Non-U.S. Gov't Trypanosoma cruzi The carbohydrate moiety of the Tc-85 surface glycoprotein from the infective trypomastigote form of Trypanosoma cruzi was analysed. Tc-85 could be metabolically labeled by incubation of the cells with d-[14C]mannose or d-[14C]glucose. Degradation techniques were performed directly on the polyacrylamide gel band containing labeled Tc-85. A mannobiose was cleaved by β-elimination and further treatment of the remaining material under conditions which liberate N-asparaginyl linkages, released a complex oligosaccharide. The presence of sialic acid was demonstrated by: mild acid hydrolysis, neuraminidase treatment and periodate oxidation under mild conditions followed by NaB3H4 reduction, hydrolysis, and detection of NANA7 by paper electrophoresis. In addition, the chromatographic behavior of the asialooligosaccharide was significantly different from that of the original sample. Galactose, mannose and glucosamine are the other monosaccharide components of the sialooligosaccharide. © 1987. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:de Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01666851_v26_n1-2_p145_Couto
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Glycoprotein carbohydrate composition
Sialic acid
Trypanosoma cruzi
Trypomastigote
glycoprotein
membrane protein
n acetylneuraminic acid
oligosaccharide
sialic acid derivative
affinity chromatography
animal
article
gel chromatography
paper chromatography
paper electrophoresis
Trypanosoma cruzi
Animal
Chromatography, Affinity
Chromatography, Gel
Chromatography, Paper
Electrophoresis, Paper
Glycoproteins
Membrane Proteins
N-Acetylneuraminic Acid
Oligosaccharides
Sialic Acids
Support, Non-U.S. Gov't
Trypanosoma cruzi
spellingShingle Glycoprotein carbohydrate composition
Sialic acid
Trypanosoma cruzi
Trypomastigote
glycoprotein
membrane protein
n acetylneuraminic acid
oligosaccharide
sialic acid derivative
affinity chromatography
animal
article
gel chromatography
paper chromatography
paper electrophoresis
Trypanosoma cruzi
Animal
Chromatography, Affinity
Chromatography, Gel
Chromatography, Paper
Electrophoresis, Paper
Glycoproteins
Membrane Proteins
N-Acetylneuraminic Acid
Oligosaccharides
Sialic Acids
Support, Non-U.S. Gov't
Trypanosoma cruzi
Couto, A.S.
Katzin, A.M.
Colli, W.
de Lederkremer, R.M.
Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi
topic_facet Glycoprotein carbohydrate composition
Sialic acid
Trypanosoma cruzi
Trypomastigote
glycoprotein
membrane protein
n acetylneuraminic acid
oligosaccharide
sialic acid derivative
affinity chromatography
animal
article
gel chromatography
paper chromatography
paper electrophoresis
Trypanosoma cruzi
Animal
Chromatography, Affinity
Chromatography, Gel
Chromatography, Paper
Electrophoresis, Paper
Glycoproteins
Membrane Proteins
N-Acetylneuraminic Acid
Oligosaccharides
Sialic Acids
Support, Non-U.S. Gov't
Trypanosoma cruzi
description The carbohydrate moiety of the Tc-85 surface glycoprotein from the infective trypomastigote form of Trypanosoma cruzi was analysed. Tc-85 could be metabolically labeled by incubation of the cells with d-[14C]mannose or d-[14C]glucose. Degradation techniques were performed directly on the polyacrylamide gel band containing labeled Tc-85. A mannobiose was cleaved by β-elimination and further treatment of the remaining material under conditions which liberate N-asparaginyl linkages, released a complex oligosaccharide. The presence of sialic acid was demonstrated by: mild acid hydrolysis, neuraminidase treatment and periodate oxidation under mild conditions followed by NaB3H4 reduction, hydrolysis, and detection of NANA7 by paper electrophoresis. In addition, the chromatographic behavior of the asialooligosaccharide was significantly different from that of the original sample. Galactose, mannose and glucosamine are the other monosaccharide components of the sialooligosaccharide. © 1987.
format JOUR
author Couto, A.S.
Katzin, A.M.
Colli, W.
de Lederkremer, R.M.
author_facet Couto, A.S.
Katzin, A.M.
Colli, W.
de Lederkremer, R.M.
author_sort Couto, A.S.
title Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi
title_short Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi
title_full Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi
title_fullStr Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi
title_full_unstemmed Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi
title_sort sialic acid in a complex oligosaccharide chain of the tc-85 surface glycoprotein from the trypomastigote stage of trypanosoma cruzi
url http://hdl.handle.net/20.500.12110/paper_01666851_v26_n1-2_p145_Couto
work_keys_str_mv AT coutoas sialicacidinacomplexoligosaccharidechainofthetc85surfaceglycoproteinfromthetrypomastigotestageoftrypanosomacruzi
AT katzinam sialicacidinacomplexoligosaccharidechainofthetc85surfaceglycoproteinfromthetrypomastigotestageoftrypanosomacruzi
AT colliw sialicacidinacomplexoligosaccharidechainofthetc85surfaceglycoproteinfromthetrypomastigotestageoftrypanosomacruzi
AT delederkremerrm sialicacidinacomplexoligosaccharidechainofthetc85surfaceglycoproteinfromthetrypomastigotestageoftrypanosomacruzi
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