Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes
Phosphatidylinositol (PtdIns) metabolism through phosphatidylinositol kinase (PIKs) activities plays a central role in different signaling pathways. In Trypanosoma cruzi, causative agent of Chagas disease, PIKs have been proposed as target for drug design in order to combat this pathogen. In this wo...
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todo:paper_01666851_v203_n1-2_p14_Gimenez2023-10-03T15:04:17Z Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes Gimenez, A.M. Gesumaría, M.C. Schoijet, A.C. Alonso, G.D. Flawiá, M.M. Racagni, G.E. Machado, E.E. Kinase activity Phosphatidylinositol-3 kinase Phosphoinositide kinase Phospholipid signaling T. cruzi epimastigotes TcPI3K 2 morpholino 8 phenylchromone adenosine calcium ion deoxycholate sodium heparin phosphatidic acid phosphatidylinositol 3 kinase phosphatidylinositol 4 phosphate kinase phosphatidylinositol 4,5 bisphosphate 3 kinase phosphatidylinositol kinase protozoal protein spermine wortmannin enzyme activator enzyme inhibitor phosphatidylinositol protozoal protein Article controlled study cytosolic fraction enzyme activity enzyme phosphorylation epimastigote molecular cloning nonhuman Northern blotting phylogeny priority journal protein expression signal transduction Trypanosoma cruzi Western blotting antagonists and inhibitors Chagas disease classification drug design enzymology growth, development and aging human metabolism parasitology phosphorylation Trypanosoma cruzi Chagas Disease Cloning, Molecular Drug Design Enzyme Activators Enzyme Inhibitors Humans Phosphatidylinositol 3-Kinases Phosphatidylinositols Phosphorylation Phylogeny Protozoan Proteins Signal Transduction Trypanosoma cruzi Phosphatidylinositol (PtdIns) metabolism through phosphatidylinositol kinase (PIKs) activities plays a central role in different signaling pathways. In Trypanosoma cruzi, causative agent of Chagas disease, PIKs have been proposed as target for drug design in order to combat this pathogen. In this work, we studied the classes of PI4K, PIPK and PI3K that could participate in signaling pathways in T. cruzi epimastigote forms. For this reason, we analyzed their enzymatic parameters and detailed responses to avowed kinase inhibitors (adenosine, sodium deoxycholate, wortmannin and LY294002) and activators (Ca2+, phosphatidic acid, spermine and heparin). Our results suggest the presence and activity of a class III PI4K, a class I PIPK, a class III PI3K previously described (TcVps34) and a class I PI3K. Class I PI3K enzyme, here named TcPI3K, was cloned and expressed in a bacterial system, and their product was tested for kinase activity. The possible participation of TcPI3K in central cellular events of the parasite is also discussed. © 2015 Elsevier B.V. All rights reserved. Fil:Schoijet, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawiá, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01666851_v203_n1-2_p14_Gimenez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Kinase activity Phosphatidylinositol-3 kinase Phosphoinositide kinase Phospholipid signaling T. cruzi epimastigotes TcPI3K 2 morpholino 8 phenylchromone adenosine calcium ion deoxycholate sodium heparin phosphatidic acid phosphatidylinositol 3 kinase phosphatidylinositol 4 phosphate kinase phosphatidylinositol 4,5 bisphosphate 3 kinase phosphatidylinositol kinase protozoal protein spermine wortmannin enzyme activator enzyme inhibitor phosphatidylinositol protozoal protein Article controlled study cytosolic fraction enzyme activity enzyme phosphorylation epimastigote molecular cloning nonhuman Northern blotting phylogeny priority journal protein expression signal transduction Trypanosoma cruzi Western blotting antagonists and inhibitors Chagas disease classification drug design enzymology growth, development and aging human metabolism parasitology phosphorylation Trypanosoma cruzi Chagas Disease Cloning, Molecular Drug Design Enzyme Activators Enzyme Inhibitors Humans Phosphatidylinositol 3-Kinases Phosphatidylinositols Phosphorylation Phylogeny Protozoan Proteins Signal Transduction Trypanosoma cruzi |
| spellingShingle |
Kinase activity Phosphatidylinositol-3 kinase Phosphoinositide kinase Phospholipid signaling T. cruzi epimastigotes TcPI3K 2 morpholino 8 phenylchromone adenosine calcium ion deoxycholate sodium heparin phosphatidic acid phosphatidylinositol 3 kinase phosphatidylinositol 4 phosphate kinase phosphatidylinositol 4,5 bisphosphate 3 kinase phosphatidylinositol kinase protozoal protein spermine wortmannin enzyme activator enzyme inhibitor phosphatidylinositol protozoal protein Article controlled study cytosolic fraction enzyme activity enzyme phosphorylation epimastigote molecular cloning nonhuman Northern blotting phylogeny priority journal protein expression signal transduction Trypanosoma cruzi Western blotting antagonists and inhibitors Chagas disease classification drug design enzymology growth, development and aging human metabolism parasitology phosphorylation Trypanosoma cruzi Chagas Disease Cloning, Molecular Drug Design Enzyme Activators Enzyme Inhibitors Humans Phosphatidylinositol 3-Kinases Phosphatidylinositols Phosphorylation Phylogeny Protozoan Proteins Signal Transduction Trypanosoma cruzi Gimenez, A.M. Gesumaría, M.C. Schoijet, A.C. Alonso, G.D. Flawiá, M.M. Racagni, G.E. Machado, E.E. Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes |
| topic_facet |
Kinase activity Phosphatidylinositol-3 kinase Phosphoinositide kinase Phospholipid signaling T. cruzi epimastigotes TcPI3K 2 morpholino 8 phenylchromone adenosine calcium ion deoxycholate sodium heparin phosphatidic acid phosphatidylinositol 3 kinase phosphatidylinositol 4 phosphate kinase phosphatidylinositol 4,5 bisphosphate 3 kinase phosphatidylinositol kinase protozoal protein spermine wortmannin enzyme activator enzyme inhibitor phosphatidylinositol protozoal protein Article controlled study cytosolic fraction enzyme activity enzyme phosphorylation epimastigote molecular cloning nonhuman Northern blotting phylogeny priority journal protein expression signal transduction Trypanosoma cruzi Western blotting antagonists and inhibitors Chagas disease classification drug design enzymology growth, development and aging human metabolism parasitology phosphorylation Trypanosoma cruzi Chagas Disease Cloning, Molecular Drug Design Enzyme Activators Enzyme Inhibitors Humans Phosphatidylinositol 3-Kinases Phosphatidylinositols Phosphorylation Phylogeny Protozoan Proteins Signal Transduction Trypanosoma cruzi |
| description |
Phosphatidylinositol (PtdIns) metabolism through phosphatidylinositol kinase (PIKs) activities plays a central role in different signaling pathways. In Trypanosoma cruzi, causative agent of Chagas disease, PIKs have been proposed as target for drug design in order to combat this pathogen. In this work, we studied the classes of PI4K, PIPK and PI3K that could participate in signaling pathways in T. cruzi epimastigote forms. For this reason, we analyzed their enzymatic parameters and detailed responses to avowed kinase inhibitors (adenosine, sodium deoxycholate, wortmannin and LY294002) and activators (Ca2+, phosphatidic acid, spermine and heparin). Our results suggest the presence and activity of a class III PI4K, a class I PIPK, a class III PI3K previously described (TcVps34) and a class I PI3K. Class I PI3K enzyme, here named TcPI3K, was cloned and expressed in a bacterial system, and their product was tested for kinase activity. The possible participation of TcPI3K in central cellular events of the parasite is also discussed. © 2015 Elsevier B.V. All rights reserved. |
| format |
JOUR |
| author |
Gimenez, A.M. Gesumaría, M.C. Schoijet, A.C. Alonso, G.D. Flawiá, M.M. Racagni, G.E. Machado, E.E. |
| author_facet |
Gimenez, A.M. Gesumaría, M.C. Schoijet, A.C. Alonso, G.D. Flawiá, M.M. Racagni, G.E. Machado, E.E. |
| author_sort |
Gimenez, A.M. |
| title |
Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes |
| title_short |
Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes |
| title_full |
Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes |
| title_fullStr |
Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes |
| title_full_unstemmed |
Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes |
| title_sort |
phosphatidylinositol kinase activities in trypanosoma cruzi epimastigotes |
| url |
http://hdl.handle.net/20.500.12110/paper_01666851_v203_n1-2_p14_Gimenez |
| work_keys_str_mv |
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1782026256420700160 |