Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry
A recent addition to the arsenal of tools for glycome analysis is the use of metabolic labels that allow covalent tagging of glycans with imaging probes. In this work we show that N-azidoglucosamine was successfully incorporated into glycolipidic structures of Plasmodium falciparum intraerythrocytic...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01666851_v182_n1-2_p88_Pinero |
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todo:paper_01666851_v182_n1-2_p88_Pinero2023-10-03T15:04:16Z Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry Piñero, T. Peres, V.J. Katzin, A. Couto, A.S. Glycolipids Metabolic oligosaccharide engineering P. falciparum glucosamine derivative glycolipid glycosphingolipid glycosylphosphatidylinositol n azidoglucosamine oligosaccharide unclassified drug article erythrocyte fluorescence analysis glycosylation mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry Plasmodium falciparum priority journal structure analysis thin layer chromatography ultraviolet radiation Chromatography, Thin Layer Erythrocytes Fluorescent Dyes Glycolipids Glycosphingolipids Glycosylation Glycosylphosphatidylinositols Mass Spectrometry Metabolic Engineering Oligosaccharides Plasmodium falciparum Staining and Labeling Plasmodium falciparum A recent addition to the arsenal of tools for glycome analysis is the use of metabolic labels that allow covalent tagging of glycans with imaging probes. In this work we show that N-azidoglucosamine was successfully incorporated into glycolipidic structures of Plasmodium falciparum intraerythrocytic stages. The ability to tag glycoconjugates selectively with a fluorescent reporter group permits TLC detection of the glycolipids providing a new method to quantify dynamic changes in the glycosylation pattern and facilitating direct mass spectrometry analyses. Presence of glycosylphosphatidylinositol and glycosphingolipid structures was determined in the different extracts. Furthermore, the fluorescent tag was used as internal matrix for the MALDI experiment making even easier the analysis. © 2012 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01666851_v182_n1-2_p88_Pinero |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Glycolipids Metabolic oligosaccharide engineering P. falciparum glucosamine derivative glycolipid glycosphingolipid glycosylphosphatidylinositol n azidoglucosamine oligosaccharide unclassified drug article erythrocyte fluorescence analysis glycosylation mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry Plasmodium falciparum priority journal structure analysis thin layer chromatography ultraviolet radiation Chromatography, Thin Layer Erythrocytes Fluorescent Dyes Glycolipids Glycosphingolipids Glycosylation Glycosylphosphatidylinositols Mass Spectrometry Metabolic Engineering Oligosaccharides Plasmodium falciparum Staining and Labeling Plasmodium falciparum |
| spellingShingle |
Glycolipids Metabolic oligosaccharide engineering P. falciparum glucosamine derivative glycolipid glycosphingolipid glycosylphosphatidylinositol n azidoglucosamine oligosaccharide unclassified drug article erythrocyte fluorescence analysis glycosylation mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry Plasmodium falciparum priority journal structure analysis thin layer chromatography ultraviolet radiation Chromatography, Thin Layer Erythrocytes Fluorescent Dyes Glycolipids Glycosphingolipids Glycosylation Glycosylphosphatidylinositols Mass Spectrometry Metabolic Engineering Oligosaccharides Plasmodium falciparum Staining and Labeling Plasmodium falciparum Piñero, T. Peres, V.J. Katzin, A. Couto, A.S. Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry |
| topic_facet |
Glycolipids Metabolic oligosaccharide engineering P. falciparum glucosamine derivative glycolipid glycosphingolipid glycosylphosphatidylinositol n azidoglucosamine oligosaccharide unclassified drug article erythrocyte fluorescence analysis glycosylation mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry Plasmodium falciparum priority journal structure analysis thin layer chromatography ultraviolet radiation Chromatography, Thin Layer Erythrocytes Fluorescent Dyes Glycolipids Glycosphingolipids Glycosylation Glycosylphosphatidylinositols Mass Spectrometry Metabolic Engineering Oligosaccharides Plasmodium falciparum Staining and Labeling Plasmodium falciparum |
| description |
A recent addition to the arsenal of tools for glycome analysis is the use of metabolic labels that allow covalent tagging of glycans with imaging probes. In this work we show that N-azidoglucosamine was successfully incorporated into glycolipidic structures of Plasmodium falciparum intraerythrocytic stages. The ability to tag glycoconjugates selectively with a fluorescent reporter group permits TLC detection of the glycolipids providing a new method to quantify dynamic changes in the glycosylation pattern and facilitating direct mass spectrometry analyses. Presence of glycosylphosphatidylinositol and glycosphingolipid structures was determined in the different extracts. Furthermore, the fluorescent tag was used as internal matrix for the MALDI experiment making even easier the analysis. © 2012 Elsevier B.V. |
| format |
JOUR |
| author |
Piñero, T. Peres, V.J. Katzin, A. Couto, A.S. |
| author_facet |
Piñero, T. Peres, V.J. Katzin, A. Couto, A.S. |
| author_sort |
Piñero, T. |
| title |
Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry |
| title_short |
Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry |
| title_full |
Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry |
| title_fullStr |
Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry |
| title_full_unstemmed |
Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry |
| title_sort |
metabolic oligosaccharide engineering of plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry |
| url |
http://hdl.handle.net/20.500.12110/paper_01666851_v182_n1-2_p88_Pinero |
| work_keys_str_mv |
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1807322818032959488 |