Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi
Calmodulin has been purified from Trypanosoma cruzi epimastigote forms by ion-exchange chromatography, gel filtration and affinity chromatography on 2-chloro-10-(3-aminopropyl)phenotiazine-Sepharose. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the factor showed a polypeptide band...
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todo:paper_01666851_v17_n2_p143_Tellezinon2023-10-03T15:04:16Z Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi Téllez-iñón, M.T. Ulloa, R.M. Torruella, M. Torres, H.N. Calcium Calmodulin Cyclic AMP Phosphodiesterase Trypanosoma cruzi adenosine phosphate calcium calmodulin enzyme phosphodiesterase 3',5' cyclic nucleotide phosphodiesterase calcium calmodulin animal cell metabolism nonhuman priority journal protozoon trypanosoma cruzi animal article brain cortex cattle comparative study enzymology isolation and purification kinetics Neurospora crassa Trypanosoma cruzi 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Calcium Calmodulin Cattle Cerebral Cortex Comparative Study Kinetics Neurospora crassa Support, Non-U.S. Gov't Trypanosoma cruzi Calmodulin has been purified from Trypanosoma cruzi epimastigote forms by ion-exchange chromatography, gel filtration and affinity chromatography on 2-chloro-10-(3-aminopropyl)phenotiazine-Sepharose. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the factor showed a polypeptide band with an apparent molecular weight of 16 000. In addition, cyclic AMP phosphodiesterase activity from T. cruzi epimastigote forms was purified by ion-exchange chromatography and affinity chromatography on a brain calmodulin-Sepharose column. The enzyme was activated by homologous calmodulin as well as by bovine brain and Neurospora crassa calmodulins. The activation required micromolar concentrations of Ca2+ and it was blocked by EGTA and by some neuroleptic drugs such as chlorpromazine, fluphenazine and compound 48 80. Activations were observed at micromolar concentrations of cyclic AMP as substrate. In addition, T. cruzi calmodulin was also active in bringing about the stimulation of brain phosphodiesterase. © 1985. Fil:Torruella, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01666851_v17_n2_p143_Tellezinon |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Calcium Calmodulin Cyclic AMP Phosphodiesterase Trypanosoma cruzi adenosine phosphate calcium calmodulin enzyme phosphodiesterase 3',5' cyclic nucleotide phosphodiesterase calcium calmodulin animal cell metabolism nonhuman priority journal protozoon trypanosoma cruzi animal article brain cortex cattle comparative study enzymology isolation and purification kinetics Neurospora crassa Trypanosoma cruzi 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Calcium Calmodulin Cattle Cerebral Cortex Comparative Study Kinetics Neurospora crassa Support, Non-U.S. Gov't Trypanosoma cruzi |
| spellingShingle |
Calcium Calmodulin Cyclic AMP Phosphodiesterase Trypanosoma cruzi adenosine phosphate calcium calmodulin enzyme phosphodiesterase 3',5' cyclic nucleotide phosphodiesterase calcium calmodulin animal cell metabolism nonhuman priority journal protozoon trypanosoma cruzi animal article brain cortex cattle comparative study enzymology isolation and purification kinetics Neurospora crassa Trypanosoma cruzi 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Calcium Calmodulin Cattle Cerebral Cortex Comparative Study Kinetics Neurospora crassa Support, Non-U.S. Gov't Trypanosoma cruzi Téllez-iñón, M.T. Ulloa, R.M. Torruella, M. Torres, H.N. Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi |
| topic_facet |
Calcium Calmodulin Cyclic AMP Phosphodiesterase Trypanosoma cruzi adenosine phosphate calcium calmodulin enzyme phosphodiesterase 3',5' cyclic nucleotide phosphodiesterase calcium calmodulin animal cell metabolism nonhuman priority journal protozoon trypanosoma cruzi animal article brain cortex cattle comparative study enzymology isolation and purification kinetics Neurospora crassa Trypanosoma cruzi 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Calcium Calmodulin Cattle Cerebral Cortex Comparative Study Kinetics Neurospora crassa Support, Non-U.S. Gov't Trypanosoma cruzi |
| description |
Calmodulin has been purified from Trypanosoma cruzi epimastigote forms by ion-exchange chromatography, gel filtration and affinity chromatography on 2-chloro-10-(3-aminopropyl)phenotiazine-Sepharose. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the factor showed a polypeptide band with an apparent molecular weight of 16 000. In addition, cyclic AMP phosphodiesterase activity from T. cruzi epimastigote forms was purified by ion-exchange chromatography and affinity chromatography on a brain calmodulin-Sepharose column. The enzyme was activated by homologous calmodulin as well as by bovine brain and Neurospora crassa calmodulins. The activation required micromolar concentrations of Ca2+ and it was blocked by EGTA and by some neuroleptic drugs such as chlorpromazine, fluphenazine and compound 48 80. Activations were observed at micromolar concentrations of cyclic AMP as substrate. In addition, T. cruzi calmodulin was also active in bringing about the stimulation of brain phosphodiesterase. © 1985. |
| format |
JOUR |
| author |
Téllez-iñón, M.T. Ulloa, R.M. Torruella, M. Torres, H.N. |
| author_facet |
Téllez-iñón, M.T. Ulloa, R.M. Torruella, M. Torres, H.N. |
| author_sort |
Téllez-iñón, M.T. |
| title |
Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi |
| title_short |
Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi |
| title_full |
Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi |
| title_fullStr |
Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi |
| title_full_unstemmed |
Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi |
| title_sort |
calmodulin and ca2+-dependent cyclic amp phosphodiesterase activity in trypanosoma cruzi |
| url |
http://hdl.handle.net/20.500.12110/paper_01666851_v17_n2_p143_Tellezinon |
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