Plasmodium falciparum biosynthesizes sulfoglycosphingolipids

Sulfated glycosphingolipids are present on the surface of a variety of cells. They are active participants in adhesion processes in many systems and appear to be involved in the regulation of cell proliferation, differentiation and other developmental cellular events. However, the body of knowledge...

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Autores principales:	Landoni, M., Duschak, V.G., Peres, V.J., Nonami, H., Erra-Balsells, R., Katzin, A.M., Couto, A.S.
Formato:	JOUR
Materias:	Glycoconjugates Malaria Plasmodium falciparum Sulfoglycosphingolipids carbon 14 glucose palmitic acid sulfatide article biosynthesis lipogenesis matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman priority journal thin layer chromatography Animals Antimalarials Carbon Radioisotopes Chromatography, Thin Layer Glucose Mass Spectrometry Morpholines Palmitic Acid Sphingolipids Sulfates Apicomplexa Protozoa
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01666851_v154_n1_p22_Landoni
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01666851_v154_n1_p22_Landoni
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spelling	todo:paper_01666851_v154_n1_p22_Landoni2023-10-03T15:04:14Z Plasmodium falciparum biosynthesizes sulfoglycosphingolipids Landoni, M. Duschak, V.G. Peres, V.J. Nonami, H. Erra-Balsells, R. Katzin, A.M. Couto, A.S. Glycoconjugates Malaria Plasmodium falciparum Sulfoglycosphingolipids carbon 14 glucose palmitic acid sulfatide article biosynthesis lipogenesis matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman priority journal thin layer chromatography Animals Antimalarials Carbon Radioisotopes Chromatography, Thin Layer Glucose Mass Spectrometry Morpholines Palmitic Acid Plasmodium falciparum Sphingolipids Sulfates Sulfoglycosphingolipids Apicomplexa Plasmodium falciparum Protozoa Sulfated glycosphingolipids are present on the surface of a variety of cells. They are active participants in adhesion processes in many systems and appear to be involved in the regulation of cell proliferation, differentiation and other developmental cellular events. However, the body of knowledge about synthesis, structure, and function of glycolipids in parasitic protozoa is very limited so far. In this work, we show by metabolic incorporation of [14C]palmitic acid, [14C]glucose and Na235SO4 that sulfoglycosphingolipids are biosynthesized in the three intraerythrocytic stages of Plasmodium falciparum. After saponification, purification of the labelled acidic components was achieved and two components named SPf1 and SPf2 were characterized. Chemical degradations and TLC analysis pointed out to sulfolipidic structures. Analysis by UV-MALDI-TOF mass spectrometry in the negative ion mode using nor-harmane as matrix showed for SPf2 a structure consisting in a disulfated hexose linked to a 20:1 sphingosine acylated with C18:0 fatty acid. Interestingly, parasite treatment with low concentrations of d,l-threo-phenyl-2-palmitoylamino-3-morpholino-1-propanol (PPMP) caused an arrest on parasite development associated to the inhibition of sulfoglycolipid biosynthesis. Taking into account that sulfoglycolipidic structures are currently involved in adhesion processes, our findings open the possibility to study the participation of this type of structures in the described aggregation phenomena in severe malaria and may contribute to clarify the pathogenesis of the disease. This report shows for the first time the synthesis of sulfoglycolipids in Apicomplexa. © 2007 Elsevier B.V. All rights reserved. Fil:Landoni, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01666851_v154_n1_p22_Landoni
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Glycoconjugates Malaria Plasmodium falciparum Sulfoglycosphingolipids carbon 14 glucose palmitic acid sulfatide article biosynthesis lipogenesis matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman priority journal thin layer chromatography Animals Antimalarials Carbon Radioisotopes Chromatography, Thin Layer Glucose Mass Spectrometry Morpholines Palmitic Acid Plasmodium falciparum Sphingolipids Sulfates Sulfoglycosphingolipids Apicomplexa Plasmodium falciparum Protozoa
spellingShingle	Glycoconjugates Malaria Plasmodium falciparum Sulfoglycosphingolipids carbon 14 glucose palmitic acid sulfatide article biosynthesis lipogenesis matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman priority journal thin layer chromatography Animals Antimalarials Carbon Radioisotopes Chromatography, Thin Layer Glucose Mass Spectrometry Morpholines Palmitic Acid Plasmodium falciparum Sphingolipids Sulfates Sulfoglycosphingolipids Apicomplexa Plasmodium falciparum Protozoa Landoni, M. Duschak, V.G. Peres, V.J. Nonami, H. Erra-Balsells, R. Katzin, A.M. Couto, A.S. Plasmodium falciparum biosynthesizes sulfoglycosphingolipids
topic_facet	Glycoconjugates Malaria Plasmodium falciparum Sulfoglycosphingolipids carbon 14 glucose palmitic acid sulfatide article biosynthesis lipogenesis matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman priority journal thin layer chromatography Animals Antimalarials Carbon Radioisotopes Chromatography, Thin Layer Glucose Mass Spectrometry Morpholines Palmitic Acid Plasmodium falciparum Sphingolipids Sulfates Sulfoglycosphingolipids Apicomplexa Plasmodium falciparum Protozoa
description	Sulfated glycosphingolipids are present on the surface of a variety of cells. They are active participants in adhesion processes in many systems and appear to be involved in the regulation of cell proliferation, differentiation and other developmental cellular events. However, the body of knowledge about synthesis, structure, and function of glycolipids in parasitic protozoa is very limited so far. In this work, we show by metabolic incorporation of [14C]palmitic acid, [14C]glucose and Na235SO4 that sulfoglycosphingolipids are biosynthesized in the three intraerythrocytic stages of Plasmodium falciparum. After saponification, purification of the labelled acidic components was achieved and two components named SPf1 and SPf2 were characterized. Chemical degradations and TLC analysis pointed out to sulfolipidic structures. Analysis by UV-MALDI-TOF mass spectrometry in the negative ion mode using nor-harmane as matrix showed for SPf2 a structure consisting in a disulfated hexose linked to a 20:1 sphingosine acylated with C18:0 fatty acid. Interestingly, parasite treatment with low concentrations of d,l-threo-phenyl-2-palmitoylamino-3-morpholino-1-propanol (PPMP) caused an arrest on parasite development associated to the inhibition of sulfoglycolipid biosynthesis. Taking into account that sulfoglycolipidic structures are currently involved in adhesion processes, our findings open the possibility to study the participation of this type of structures in the described aggregation phenomena in severe malaria and may contribute to clarify the pathogenesis of the disease. This report shows for the first time the synthesis of sulfoglycolipids in Apicomplexa. © 2007 Elsevier B.V. All rights reserved.
format	JOUR
author	Landoni, M. Duschak, V.G. Peres, V.J. Nonami, H. Erra-Balsells, R. Katzin, A.M. Couto, A.S.
author_facet	Landoni, M. Duschak, V.G. Peres, V.J. Nonami, H. Erra-Balsells, R. Katzin, A.M. Couto, A.S.
author_sort	Landoni, M.
title	Plasmodium falciparum biosynthesizes sulfoglycosphingolipids
title_short	Plasmodium falciparum biosynthesizes sulfoglycosphingolipids
title_full	Plasmodium falciparum biosynthesizes sulfoglycosphingolipids
title_fullStr	Plasmodium falciparum biosynthesizes sulfoglycosphingolipids
title_full_unstemmed	Plasmodium falciparum biosynthesizes sulfoglycosphingolipids
title_sort	plasmodium falciparum biosynthesizes sulfoglycosphingolipids
url	http://hdl.handle.net/20.500.12110/paper_01666851_v154_n1_p22_Landoni
work_keys_str_mv	AT landonim plasmodiumfalciparumbiosynthesizessulfoglycosphingolipids AT duschakvg plasmodiumfalciparumbiosynthesizessulfoglycosphingolipids AT peresvj plasmodiumfalciparumbiosynthesizessulfoglycosphingolipids AT nonamih plasmodiumfalciparumbiosynthesizessulfoglycosphingolipids AT errabalsellsr plasmodiumfalciparumbiosynthesizessulfoglycosphingolipids AT katzinam plasmodiumfalciparumbiosynthesizessulfoglycosphingolipids AT coutoas plasmodiumfalciparumbiosynthesizessulfoglycosphingolipids
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Plasmodium falciparum biosynthesizes sulfoglycosphingolipids

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